TPP PCV Packed Cell Volume Tube Instruction Manual

TPP PCV Packed Cell Volume Tube Instruction Manual

Instruction for Use TPP PCV tube (Packed Cell Volume)

TPP „easy read“ Measuring Device

The PCV tube, together with the “easy read” measuring device, allows easy and fast measurement of biomass in cell culture, microbiology and suspension cultures of fungi and yeasts. You need a centrifuge with swingout rotor. The PCV tube is intended for single use, the “easy read” measuring device is reusable.

Note

Follow national regulations when handling biological material, use appropriate protective clothing Take note of the regulations of aseptic working during the work process.

Instructions PCV tube

• Check the packaging material and the product for intactness. Use only faultless material.
• Use only PCV tubes with a valid expiration date (EXP).
• Transfer a sample of 100 – 1000 µl of a mixed cell suspension into the PCV cell measuring tube.
• Close the tube with the cap #87008 if necessary.
• Centrifuge the tube for 1 minute at 2’500 x g in a swing-out rotor for 1.5 | 2 mL vessels.
• Read the volume of the cell pellet as described in the TPP “easy read” measuring instrument instructions.
• Discard the PCV tube

Instruction “easy read” Measuring Device

• After centrifugation, insert the PCV tube① into the opening provided in the “easy read” after centrifugation.
• Move the magnifying glass ③ unƟl the height of the cell pellet in the capillary is equal to the cross.
• Read the cell pellet volume in μl with the “easy read” measuring device.

Cleaning and disinfection of the “easy read

Please note the following when choosing the cleaning and disinfecting agent:

• Choose procedures with neutral, aluminumcompatible cleaning and disinfecting agents and fully demineralized water.
• Avoid abrasive cleaning agents or scratching metal brushes, agents containing halogen orchloride: risk of corrosion.
• Thermal cleaning (autoclaving) is not recommended.
• Remove all cleaning agent residues by rinsing thoroughly.
• After cleaning/disinfection, dry the instrument completely and store it in a dry place.

Comments and recommendations:

• The PCV method does not provide information about the cell viability of the culture.
• A new calibration line must be determined for each cell type.
• Cell pellet cannot be removed from the capillary.

Calculation PCV (%) value:

• The measured volume is expressed in % of the volume of the transferred suspension sample. It corresponds to the total biomass of the culture.
  (%) = × 100

Calculation of cell density:

To determine the cell density from the biomass (PCV %) a calibration line must be established.

1. Take a 2 ml sample from a well mixed cell suspension.
2. Carry out a double determination for the PCV value (%) and for the cell density using the counting chamber (cell number/ml).
3. The frequency of sampling and measurements performed depends on the growth rate (doubling time) of the cells.
4. Enter the determined mean values in a table and create a diagram (see Fig. 2).
5. Determine the linear equation based on the measured values (Fig. 2)

y = mx + b
m = Increase of the straight line
y = PCV %
x = Cell density cell number/mL
b = ordinate section

7. The cell density of a sample cell number/ml can be calculated from the determined straight line equation:

= ( − )

A typical calibration line for a cell line (NS0) which correlates the cell count per millilitre (determined by microscopic counting) with the biomass value of the sample expressed in percent.

PCV Tube

**Centrifuges and Rotor***

Technical Data:

Materials

• Measuring device aluminum
• Magnifying lens glass

Magnifying lens:
Length x width x height mm| 30 x 33 x 3

Additional

Instructions for use, chemical resistance lists, and quality certificates of the respective products are available for download on the homepage www.tpp.ch

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