TPP TubeSpin Bioreactor Instructions
- June 3, 2024
- TPP
Table of Contents
TubeSpin Bioreactor
Instructions
TubeSpin Bioreactor
The TubeSpin® bioreactor is suitable for high throughput screening,
cultivation and optimization processes of prokaryotic (aerobic bacteria) and
eukaryotic (yeasts, algae, HEK, CHO, Sf-9) suspension cells. The TubeSpin®
bioreactor is available with a filter screw cap with 5 or 10 openings over a
PTFE filter membrane. The TubeSpin® Bioreactor is intended for single use
only.
Note: Follow national regulations when handling biological material, use
appropriate protective clothing Take note of the regulations of aseptic
working during the work process.
Instructions
-
Check the packaging material and the product for intactness. Use only faultless material.
-
Check the expiry date (EXP) on label and packaging. Only use products with valid EXP.
-
Open the packaging in a sterile environment and remove an entire system for use.
-
Open the bioreactor and fill it with the medium and inoculum according to your laboratory routine. Please refer to the optimal filling quantity (see technical data).
-
Close the filter screw cap with ventilation holes. Continuous gas exchange takes place through the integrated hydrophobic membrane 0.22 µm.
-
If the aeration/gas exchange needs to be reduced, individual openings may be closed using lab-appropriate labels or tape (picture 1) Picture 1.
-
Cultivate: Shake cells in appropriate incubation shakers. Refer to the cultivation conditions of the cells (temperature, CO2 and humidity control, shaking frequency.
-
Shakers: Follow the safety instructions and operating instructions of the shaker manufacturer.
-
Centrifuging: The use of suitable rotors or centrifuge adapters is recommended. Follow the safety instructions of the centrifuge manufacturer.
-
The mechanical strength of the tubes during centrifugation is influenced by o Shape and material
o Accuracy of fit in the centrifuge adapter
o Temperature, centrifugation time, g-number
o Chemical and physical properties of the centrifuged material o Rotor type: fixed-angle or swing-out rotor -
Test the multitude of influencing factors under routine conditions beforehand.
-
Storage before use at room temperature, protected from UV light
Remarks and Recommendations
- The graduation serves as a guide for filling quantity and is no absolute value.
- Alcohol 90 % with mechanical stress (rubbing) might dissolve or blur the white color of the print.
- For freezing, TPP recommends transferring samples to the TPP cryo tubes. If TubeSpin® bioreactor storage in the minus temperature range is desired, TPP recommends testing the product beforehand according to your conditions.
- TPP assumes no liability for damage caused by, during or due to the use of centrifuge tubes in freezing processes.
Technical Data
Materials
| Screw Cap | PE |
|---|---|
| Membrane | PTFE |
| Tube | PP |
Additional: Instructions for use, chemical resistance lists,and quality certificates of the respective products are available for download on the homepage www.tpp.ch.
| Measurements | 87015 | 87017 | 87050 | 87056 |
|---|---|---|---|---|
| Volume grad. mL | 15 | 15 | 50 | 50 |
| Length mm | 120 | 120 | 115 | 115 |
| Diameter mm | 17.1 | 17.1 | 30 | 30 |
| Max. RCF x g | 15‘500 | 15‘500 | 15‘500 | 15‘500 |
| Form | conical | round | conical | round |
| Optimal | 1 – 10 | 1 – 10 | 1 – 35 | 1 – 35 |
| Shaker: | 50 | 50 | 50 | 50 |
| Shaker: | 250 | 250 | 180 | 180 |
| Measurements | 87450 | 87600 | ||
| --- | --- | --- | ||
| Volume grad. mL | 450 | 600 | ||
| Length mm | 145 | 181 | ||
| Diameter mm | 96 | 98 | ||
| Max. RCF x g | 3‘500 | 3‘500 | ||
| Form | conical | conical | ||
| Optimal filling volume mL | >300 | >400 | ||
| Shaker & Screw Cap | 87450 | 87600 | ||
| Shaker: | 50 | 50 | ||
| haker: Recom. speed | 150 | 150 | ||
| Screw cap „solid“ | 87355 | 87355 | ||
| Screw cap „filter“ | 87356 | 87356 |
Aberturas / Openings Ø mm
| 87015 / 87017| 87050 / 8705| 87450 / 87600
---|---|---|---
A| 0.4| 0.4| –
B| 0.6| 0.6| –
C| 1| 1| –
D| 1.25| 1.5| –
E| 1.5| 2| –
10 x cada| –| –| 4
Note: TPP products are for research use only and not intended for use in clinical, diagnostic or therapeutic procedures. TPP does not assume any guarantee for
TPP Techno Plastic Products AG
Zollstrasse 7, CH-8219 Trasadingen, Switzerland
Tel. +41 (0)52 687 01 87,
Fax +41 (0)52 687 01 77
info@tpp.ch, www.tpp.ch