TPP Z707538 Tissue Culture Flasks Instructions

TPP Z707538 Tissue Culture Flasks

Instructions for Use

TPP Tissue Culture Flasks with

Re-closable Lid
TPP tissue culture flasks with re-closable lid are designed for the cell/tissue cultivation and growth in manual use. They meet the need for a large access from the above to the cell culture. The growth surface is opto- mechanically activated to optimize cell adhesion and proliferation. The angled flask neck reduces the risk of medium contact with the inside of the screw cap and allows excellent access for tissue culture scrapers and serological pipettes.
TPP cell culture flasks with re-closable lid are available with filter screw caps only, when using a VENT screw cap a possible pressure build-up of 0.03 bar or higher could press open the re-closable lid. The TPP tissue culture flask with re-closable lid is intended for single use.

Note:

• Follow national regulations when handling biological material, use appropriate protective clothing
• Take note of the regulations of aseptic working during the work process.

Instructions:

• Check the packaging material and the product for intactness. Use only faultless material.

• Check the expiry date (EXP) on the label and packaging. Only use products with valid EXP.
  Note : TPP products are for research use only and not intended for use in clinical, diagnostic or therapeutic procedures. TPP does not assume any guarantee for damages and defects resulting from unregistered or improper use.

• Open the packaging in a sterile environment and remove a complete system for use.

• Check that the re-closable lid is tightly closed. During transport, storage and/or movement, it could lift minimally.

• Open the filter screw cap of the cell culture flask and fill the flask with medium and inoculum according to your standard laboratory routine.

• Close the filter screw cap completely. A continuous gas exchange takes place through the integrated hydrophobic membrane 0.22 µm. If the PTFE filter membrane gets wet, the gas exchange is reduced for a short time. Cultivate and harvest cells according to your common laboratory routine.

• Medium exchange and cell harvesting can be performed through the flask neck or the large opening from above.

• To achieve an uniform cell growth, the following aspects must be observed:

  • Resuspend the cells well with the medium to avoid cell lumps.
  • Avoid foaming during resuspension and seeding to avoid holes in the cell layer.
  • Low medium volume leads to meniscus formation and thus to uneven growth of the cells (cell clumps at the edge). Please refer to the technical data for the optimum filling volume.

• Air vents in the bottom rim ensure optimum heat distribution in the incubator when several flasks are stacked on top of each other.
  Note : TPP products are for research use only and not intended for use in clinical, diagnostic or therapeutic procedures. TPP does not assume any guarantee for damages and defects resulting from unregistered or improper use.

• The mechanical strength of the flasks during centrifugation is influenced by

  • Shape and material
  • Accuracy of fit in the centrifuge adapter
  • Temperature, centrifugation time, g- number
  • Chemical and physical properties of the centrifuged material
  • Rotor type : fixed-angle or swing-out rotor

• Test the multitude of influencing factors under routine conditions beforehand.

• Storage before use at room temperature, protect from UV light.

Opening Mechanism

The lid can be opened and closed multiple times and remain gas- and liquid- tight.

1. Press here
2. Lift out lid

Important: To do this, it is necessary to place the flask on a firm surface. To open the lid, press the notch provided for this purpose with your forefinger. This allows you to control the pressure of your finger on the opening mechanism. Too much force can lead to breakage.

Advice and Recommendations

• There are inscription fields on both sides of the flask for labelling.
• The graduation serves as a reference for the filling quantity and is not an absolute value.
• The stacking rim allows a stable stacking of several flasks on top of each other, also in combination with other TPP flasks of the same size.
• For a homogenous cultivation of the cells, levelling of the incubator and continuous supply of medium is essential.
• Avoid and/or eliminate vibration sources near the incubator to ensure uniform cell growth.
• Always check that the re-closable lid is correctly closed before using it for the first time or after opening it.

Note:
The barrier limits the growth area to 115 cm2.

Technical Data

Materials

• Flask PS
• Screw cap PE
• Membrane PTFE Pore size 0.22 um
• Sealing ring Non-toxic gasket

Additional

Instructions for use, chemical resistance lists, and quality certificates of the respective products are available for download on the homepage www.tpp.ch.

Note : TPP products are for research use only and not intended for use in clinical, diagnostic or therapeutic procedures. TPP does not assume any guarantee for damages and defects resulting from unregistered or improper use.

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