TPP IFU Tissue Culture Dish Instructions

TPP IFU Tissue Culture Dish

Instruction for Use

TPP Tissue Culture Dish
TPP tissue culture dishes are for the cell/tissue cultivation and growth in manual use. The circumferential gripping ring allows a secure grip of the unit (bowl + lid). The growth surface – but not the side wall of the dish – is opto-mechanically activated for optimal cell adhesion and growth.
The tissue culture dish is for single use.

Note:
Follow national regulations when handling biological material, use appropriate protective clothing
Take note of the regulations of aseptic working during the work process.

Instructions

• Check the packaging material and the product for intactness. Use only faultless material.

• Check the expiry date (EXP) on label and packaging. Only use products with valid EXP.

• Open the packaging in a sterile environment and remove a complete system (lid with base) for use. The circumferential grip ring allows a secure grip of the cell culture dish and thus avoids unintentional lifting of the lid (risk of contamination).

• Remove the lid and fill the dish with the medium and inoculum according to your laboratory routine.

• To achieve an uniform cell growth, the following aspects must be observed:

• Resuspend the cells well with the medium to avoid cell lumps.

• Avoid foaming during resuspension and seeding to avoid holes in the cell layer.

• Low medium volume leads to meniscus formation and thus to uneven growth of  the cells (cell clumps at the edge). Please refer to the technical data for the optimum filling volume.

• The spacer cams on the inside of the lid ensure a constant and optimal gas exchange with low evaporation.

• Tip: For sowing, medium change, sub cultivation and other activities the lid should be opened only ~45° to avoid contamination (Figure 1)

• Air vents in the bottom rim ensure optimum heat distribution in the incubator even when several dishes are stacked on top of each other.

• For identification use the labelling fields.

• As an orientation aid, for example during microscopy, the numbers in the clock system 3, 6, 9, 12 are located in the bottom of the dish (Figure 2).

• Before use, store at room temperature, protect from UV light

• Test the multitude of influencing factors under routine conditions beforehand.

Advice and Recommendations

• The stacking rim provides a safe stability of several TPP cell culture dishes stacked on top of each other.
• For a homogenous cultivation of the cells, levelling of the incubator and continuous supply of medium is essential.
• Avoid and/or eliminate vibration sources near the incubator to ensure uniform cell growth.
• Centrifugation of the dish is not recommended.

Technical Data

• Materials Dish: PS
• Lid; PS
  Measurements| 93040| 93060| 93100| 93150
  ---|---|---|---|---
  Outside-  mm| 40| 60| 96| 146
  Height mm| 11| 16| 21| 21
  Inside-  mm| 34| 53| 87| 137
  Volume recom. mL| 2 – 3| 3 – 6| 10 – 17| 20 – 42
  Growth surface cm 2| 9.2| 22.1| 60.0| 147.0

Tissue Scraper and Tissue Spatula

Technical Data

• Materials:
• Handle; PE
• Scraper head: PE
  Measurements| 99002| 99003| 99004| 99010
  ---|---|---|---|---
  Length mm| 240| 300| 380| 195
  Width of head mm| 13| 20| 25| 14
  Scraper rotational| R| R| R| Espátula

Additional

Instructions for use, chemical resistance lists, and quality certificates of the respective products are available for download on the homepage www.tpp.ch.

Source: TPP/literature

Note: TPP products are for research use only and not intended for use in clinical, diagnostic or therapeutic procedures. TPP does not assume any guarantee for damages and defects resulting from unregistered or improper use.
TPP recommendations are guidelines and do not represent an absolute value. TPP accepts no responsibility for the topicality, accuracy, completeness or quality of this IFU.

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