TPP 93040 Tissue Culture Dish Instruction Manual

TPP 93040 Tissue Culture Dish

Instruction for Use

TPP tissue culture dishes are for the cell/tissue cultivation and growth in manual use. The circumferential gripping ring allows a secure grip of the unit (bowl + lid). The growth surface – but not the side wall of the dish – is optomechanically activated for optimal cell adhesion and growth. The tissue culture dish is for single use.

Note: Follow national regulations when handling biological material, use appropriate protective clothing Take note of the regulations of aseptic working during the work process.

Instructions

• Check the packaging material and the product for intactness. Use only faultless material.

• Check the expiry date (EXP) on label and packaging. Only use products with valid EXP.

•  Open the packaging in a sterile environment and remove a complete system (lid with base) for use. The circumferential grip ring allows a secure grip of the cell culture ish and thus avoids unintentional lifting of the lid (risk of contamination).

• Remove the lid and fill the dish with the medium and inoculum according to your laboratory routine.

• To achieve an uniform cell growth, the following aspects must be observed:

  • Resuspend the cells well with the medium to avoid cell lumps.
  • Avoid foaming during resuspension and seeding to avoid holes in the cell layer.
  • Low medium volume leads to meniscu formation and thus to uneven growth of the cells (cell clumps at the edge). Please refer to the technical data for the optimum filling volume.

• The spacer cams on the inside of the lid ensure a constant and optimal gas exchange with low evaporation.

• Tip: For sowing, medium change, sub cultivation and other activities the lid should be opened only ~45° to avoid contamination (Figure 1)

• Air vents in the bottom rim ensure optimum heat distribution in the incubator even when several dishes are stacked on top of each other.

• For identification use the labelling fields.

• As an orientation aid, for example during microscopy, the numbers in the clock system 3, 6, 9, 12 are located in the bottom of the dish (Figure 2).

• Before use, store at room temperature, protect from UV light

• Test the multitude of influencing factors under routine conditions beforehand.

Advice and Recommendations:

• The stacking rim provides a safe stability of several TPP cell culture dishes stacked on top of each other
• For a homogenous cultivation of the cells, levelling of the incubator and continuous supply of medium is essential.
• Avoid and/or eliminate vibration sources near the incubator to ensure uniform cell growth
• Centrifugation of the dish is not recommended.

Technical Data

Materials

• Dish PS
• Lid PS

Tissue Scraper and Tissue Spatula

Technical Data

Materials

• Handle PE
• Scraper head PE

Additional:
Instructions for use, chemical resistance lists, and quality certificates of the respective products are available for download on the homepage www.tpp.ch.

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