TPP 89012 Cryo Tube Instruction Manual
- September 3, 2022
- TPP
Table of Contents
TPP 89012 Cryo Tube
TPP cryo tubes are made of polypropylene (PP) with external thread and star feet are designed for storage of biological material and samples down to -196°C. The screw caps with external thread reduce the risk of contamination and cross-contamination. The cryo tube is intended for single use.
Note:
Follow national regulations when handling biological material, use appropriate
protective clothing Take note of the regulations of aseptic working during the
work process. TPP recommends storing the tubes exclusively in the gas phase of
nitrogen (LN2). If stored in theliquid phase, LN2 could penetrate. During
thawing process, the rapid evaporation would then cause a build-up of internal
pressure. This could result in an explosion of the tube and release of
biologically hazardous substances.
Instructions
- Check the packaging material and the product for intactness. Use only faultless material.
- Check the expiry date (EXP) on label and packaging. Only use products with valid EXP.
- Open the packaging in a sterile environment and remove a complete system for use.
- For a firm and safe stand during work, place the cryotubes in the appropriate rack (#99016).
- Remove the cap and fill according to your laboratory routine. Please refer to the recommended 80 % and maximal 90 % filling quantity to allow the sample volume to expand.
- Close the screw cap firmly. The thread must be dry to close the tube, moisture will affect the tightness. When closing the screw cap, avoid overtightening it and thus damaging the sealing lip.
- Use only cryo containers (or freezers) intended for freezing processes. Unsuitable boxes and racks have an insulating effect and can lead to uneven freezing, resulting in loss of cell vitality and/or damage to the tube.
- Centrifuging: The use of appropriate rotors and/or centrifuge adapters is recommended. Please follow the safety instructions of the centrifuge manufacturer.
- The mechanical load capacity of the centrifuge tubes is influenced by
- Shape and material
- Accuracy of fit in the centrifuge adapter
- Temperature, centrifugation time, g-number
- Chemical and physical properties of the centrifuged material
- Rotor type: fixed-angle or swing-out rotor
- Test the multitude of influencing factors under routine conditions beforehand.
- Before use, store at room temperature and protect from UV light.
Note: TPP products are for research use only and not intended for use in clinical, diagnostic or therapeutic procedures. TPP does not assume any guarantee for damages and defects resulting from unregistered or improper use.
Storage in Minus Temperatures
- When storing the tube in the minus temperature range, first test the multitude of influencing factors under routine conditions.
- Temperatures below 0 °C result in reduced elasticity for polypropylene. Do not stress frozen tubes mechanically such as dropping or hitting. Periodically test under routine conditions for potential issues that could damage your samples.
- Only use “freezing containers” for controlled freezing of cells before converting the cryotubes into the gas phase of a nitrogen tank.
Advice and Recommendations
- The graduation serves as a reference for the filling quantity and is not an absolute value.
- A labeling area and colored cap inserts allow the identification of samples.
- A detailed freezing protocol for cells can be found on our homepage www.tpp.ch
Accessories
- Cryo Box L, # 99014 / Cryo Box S, # 99015
- Cryo Rack # 99016: The star foot in combination with the TPP rack allows one-handed operation.
- Colored cap inserts for identification
Technical Data
Materials
- Screw cap PP
- Tube PP
Instructions:
Designed for storage of cryo tubes and most other similarly designed vials in
ultralow temperature. Cryo Box L, # 99014 / Cryo Box S, # 99015 Cryo Rack #
99016
Additional:
Instructions for use, chemical resistance lists, and quality certificates of
the respective products are available for download on the homepage
www.tpp.ch.