GALENVS MagnetiQ Blood & Cell Extraction Kit Instructions
- June 3, 2024
- GALENVS
Table of Contents
GALENVS MagnetiQ Blood & Cell Extraction Kit
Introduction
The Galenvs magnetiQ Blood & Cell DNA Extraction Kit enables a rapid and efficient method of genomic DNA (gDNA) isolation and purification from whole blood samples and mammalian cell suspensions. Our magnetic bead technology allows for a combined lysis and capture step and efficient washing steps leading to high yields of concentrated gDNA free of cell debris, salts, lipid, or protein contamination under 20 minutes.
Applications
- Next Generation Sequencing (NGS)
- PCR and qPCR gene amplification
- DNA methylation studies
- Copy number variation (CNV) studies
Features
- Amenable to high-throughput methods and automation (Kingfisher Flex, Allsheng AutoPure96, etc.)
- Faster and simpler magnetic collection and resuspension steps
- Reduced clogging and contaminant/cell debris carryover
- Eliminates organic solvent hazardous waste
- Requires only the use of a magnetic rack and standard pipettes
- Does not require centrifugation steps
- Storage and operation at room temperature
Performance
Yield and Purity
In comparing the Galenvs magnetiQ Blood & Cell kit to industry-leading
products (DNeasy Blood & Tissue by Qiagen), 100 uL of whole human blood
(Potassium/EDTA stabilized) are processed as described by manufacturer-
recommended protocols (Figure 1).
Figure 1: Extraction yield of gDNA from 100 uL of whole blood using
Galenvs magnetiQ Blood/Cell kit and Qiagen DNeasy Blood & Tissue kit.
Quantification of gDNA performed using Qubit BR DNA fluorescent assay
measurement (n=3)
Table 1: A260/A280 purity ratios of eluted gDNA samples from 100 uL whole
blood measured by UV/Vis nanodrop (n=3)
- Galenvs 1.83 +- 0.03
- Qiagen 2.04 +- 0.12
qPCR
Both quantitative PCR (qPCR) (Figure 2) and gel electrophoresis results
(Figure 3) confirm the higher recovery yields obtained with Qubit BR DNA
assay. Eluted gDNA is added to the qPCR master mix (NEB Luna) containing
primers for pre-GAPDH housekeeping gene.
Figure 2: Amplification of the pre-GAPDH gene by qPCR from 100 uL of whole blood comparing Galenvs and Qiagen extraction kits
Figure 3: Gel electrophoresis of extracted gDNA from 100 uL whole blood samples (4x) comparing Galenvs and Qiagen kits. Galenvs isolated gDNA is higher molecular weight while Qiagen extraction shows DNA smears below gDNA bands
Sample Volume
To illustrate the scalability of the Galenvs protocol, higher volumes of
whole blood were processed while scaling the lysis buffer volume accordingly.
The elution volumes can be further tuned to obtain the desired final
concentration of isolated gDNA. Figures 4 and 5 show the yield and purity,
respectively, from varying volumes of whole blood. The total yield of gDNA
increases linearly and matches the scaling factor of processed whole blood
volume – while maintaining high purity ratios. This is also confirmed in the
gel electrophoresis analysis (Figure 6).
Figure 6: Gel electrophoresis of extracted gDNA from 100 uL, 300 uL, and 1000 uL of whole blood samples processed with Galenvs magnetiQ kit
Specifications
- galenvs.com Blood, Cells, Bacteria
- Quantity 100 or 250 assays / Prefilled plates (16-96 preps)
- Elution Volume 100 μl
- Processing Mode Automated – Manual
- Sample Volume 100 μL – 1000 μL
- Binding Technology Magnetic beads
- Binding Capacity Scalable
- Storage Room temperature
Product codes
- 100 preps BC0100
- 250 Preps BC0250
- Prefilled plates 96-well BC0096
- Prefilled plates 16-well BC0016
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References
Read User Manual Online (PDF format)
Read User Manual Online (PDF format) >>