Galenvs magnet Quick Plasmid Miniprep Kit User Guide
- May 15, 2024
- GALENVS
Table of Contents
Galenvs magnet Quick Plasmid Miniprep Kit
Product Information
The Quick Plasmid Miniprep Kit is designed for rapid isolation and purification of plasmid DNA from bacterial cultures. It utilizes a column- based system and specific buffers to efficiently extract plasmid DNA for downstream applications.
Specifications
- Product Name: Quick Plasmid Miniprep Kit
- Version: QPMKIT PROTOCOL V1.0
Product Usage Instructions
Preparation
- Ensure the GPL-2 Buffer is well mixed by vortexing.
- Place the plate into the miQron system with the label facing outward.
- Insert two combs into the plate.
Protocol Steps
- Bead Transfer:
- Column Volume: 200
- Time: 30 sec
- Mixing Speed: 5
- Dry Time: 0 sec
- Magnet Capture Time: 60 sec
Note: Columns 6 and 12 contain the purified DNA elution.
Cleaning Up
Once the program is complete, remove the plate from the miQron and discard the
combs.
Frequently Asked Questions (FAQ)
Q: How do I update the protocol in the miQron system?
A: Press the Scan Protocol icon from the Run menu (protocol list view window)
and use the scanner on the provided QR code.
Overview Description
QPMKit miQron protocol parameters
Using Instruction
-
Add 800µl of GPL-1 Buffer to the GPL-2 Buffer tube.
Ensure the GPL-2 Buffer is mixed well by vortexing.
The unused mixture should be kept at 4°C for storage. -
Pellet 1–10ml of bacterial cell sample.
- Resuspend bacterial pellet in 860µl of Lysis Buffer, then add 40µl of GPL-2 Buffer.
- For 10ml bacterial culture, add 80µl of GPL-2
- Buffer for better lysis.
-
Mix well by pipetting up and down until the solution is homogenous. Incubate for 5 min at room temperature.
-
Add the Lysis Buffer (columns 1 and 7).
-
Place plate into the miQron, taking care that the label is facing outward.
-
Insert two combs.
-
Select the QPMKit protocol and press When the program is complete, remove the plate from miQron and discard the combs.
Columns 6 and 12 contain the purified DNA elution.
Protocol Update
To import the updated protocol into the miQron, press the Scan Protocol icon from the Run menu (protocol list view window). Use the scanner on the QR code below.
QPMKIT PROTOCOL V1.0
Read User Manual Online (PDF format)
Read User Manual Online (PDF format) >>