GALENVS 20220407 Viral RNA Extraction Kit User Guide
- June 3, 2024
- GALENVS
Table of Contents
Viral RNA
Extraction Kit
Quick Start Guide
GALENVS 20220407 Viral RNA Extraction Kit
****Before first use, add ethanol ( >95%) to Wash Buffer #2 as per label
instructions.
Prior to extraction, mix bottles well by inverting upside down several times.
-
In 1.5ml microfuge tube, add 100μl of sample (swab, saliva, cell-free body fluids, viral transportation media (VTM), inactive transport media (ITM), culture supernatants,or bronchoalveolar lavage fluid).
-
Add 400μl of Lysis/Binding Buffer and mix well by pipetting up-down 10–15x.
Incubate 5 mins to allow for lysis and RNA binding. -
Place tube on magnetic rack for 1–2 mins to capture RNA-bead complex, then discard supernatant.
-
Remove tube from magnetic rack and resuspend RNA–bead complex in 600μl of Wash Buffer #1.
Return to magnetic rack for 1–2 mins, then discard supernatant.
-
Repeat wash with 600μl of Wash Buffer #2, return to magnetic rack for 1–2 mins, then discard supernatant and leave to dry for 1 min.
-
Remove tube from magnetic rack and resuspend RNA–bead complex in 50μl of Elution Buffer.
Mix well by pipetting up-down 15–20x to elute RNA from beads and let stand for 1–2 mins. -
Place tube on magnetic rack to separate beads (~1–2 mins).
-
Transfer clean RNA solution (supernatant) to clean tube.
VR Viral RNA Extraction Kit
VR 1010-12
VR0250-12
Documents / Resources
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GALENVS 20220407 Viral RNA Extraction
Kit
[pdf] User Guide
20220407 Viral RNA Extraction Kit, 20220407, Viral RNA Extraction Kit, RNA
Extraction Kit, Extraction Kit
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