GALENVS Plant DNA Extraction Kit User Guide
- May 15, 2024
- GALENVS
Table of Contents
GALENVS Plant DNA Extraction Kit
Product Description
| 200µl sample added to Binding Buffer
---|---
| 100µl extracted and purified gDNA elution
| 200µl sample added to Binding Buffer
| 100µl extracted and purified gDNA elution
| Binding Buffer (600µl) Columns 1 & 7
| Wash #1 Buffer (600µl) Columns 2 & 8, 3 &9
| Wash #2 Buffer (600µl) Columns 4 & 10, 5 & 11
| Elution Buffer (100µl) Columns 6 & 12
PD Kit miQron protocol parameters
Step Name| Column| Volume (µl)| Time (sec)| Mixing Speed (1–10)| Dry Time
(sec)| Temp (°C)| Magnet Capture Time (sec)
---|---|---|---|---|---|---|---
Binding| 1 & 7| 900| 300| 7| 0| | 225
Wash #1| 2 & 8| 600| 180| 7| 0| | 135
Wash #1| 3 & 9| 600| 60| 7| 0| | 135
Wash #2| 4 & 10| 600| 60| 7| 0| | 135
Wash #2| 5 & 11| 600| 60| 7| 120| | 135
Elution| 6 & 12| 100| 60| 10| 0| 60| 180
Discard Comb| 2 & 8| 600| 0| 5| 0| | 0
Instructions For Use
-
Add up to 50mg of plant leaves sample to the lysis bead tube provided.
-
Add 500µl Lysis Buffer, 60µl PPB, and 5µl RNase.
Mix for 3 mins using Tissue Laser at max speed or vortex for 10 mins; then centrifuge at 20,000g for 2 mins.
-
Remove the protective foil.
-
Avoiding pellets, transfer up to 200µl of supernatant to Binding Buffer (Columns 1 & 7).
You can add up to 16 samples. -
Place plate into the miQron, taking care that the label is facing outward.
-
Insert two combs.
-
Select the PD Kit protocol and press
Columns 6 and 12 contain the purified DNA elution.
Plant DNA Extraction Kit
Protocol Update
To import the updated protocol into the miQron, press the Scan Protocol icon
from the Run menu (protocol list view window). Use the scanner on the QR code
below.
PDKIT PROTOCOL V1.0
Documents / Resources
|
GALENVS Plant DNA Extraction
Kit
[pdf] User Guide
Plant DNA Extraction Kit, DNA Extraction Kit, Extraction Kit, Kit
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Read User Manual Online (PDF format)
Read User Manual Online (PDF format) >>