GALENVS SD0050-12 Soil DNA Extraction Kit User Guide

SD0050-12 Soil DNA Extraction Kit
User Guide

SD0050-12 Soil DNA Extraction Kit

1. Add up to 250g of soil sample to the lysis bead tube provided.
   Add 900μl of Lysis Buffer, mix for 10 mins using TissueLyser at max speed, then centrifuge at 20,000g for 1 min.

2. Avoiding pellet, transfer up to 400–500μl of supernatant to clean centrifuge tube.
   Add 200μl of IR buffer, vortex for 10–20s, then centrifuge for 1 min.

3. Avoiding pellet, transfer up to 400–600μl of supernatant to clean centrifuge tube.
   Add 1000μl of Binding Buffer, vortex for 10–20s, and wait 5 mins.

4. Place tube on magnetic rack to capture.
   Wait 1 min then discard supernatant.

5. Add 600μl of Wash Buffer #1 to the tube and vortex for 10–20s.
   Wait 1 min then place tube on magnetic rack to capture. Wait 1 min then discard supernatant.

6. Add 600μl of Wash Buffer #2 to the tube and vortex for 10–20s. Place tube on magnetic rack to capture. Wait 1 min then discard supernatant.
   Repeat step 6 three times.
   **** Make sure to remove residual Wash #2 remaining in tube, or use drying step before elution.

7. Add 100μl of Elution Buffer to tube and mix briefly. Wait 1 min.
   Place tube on magnetic rack to capture.
   **** For increased yield heat Elution Buffer at 60°C for 5 mins.

8. Wait 1 min then transfer supernatant to clean microfuge tube.

SD  Soil DNA Extraction Kit
SD010 0-12
SDO 25 0-12

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