Galenvs PD0050 Plant DNA Extraction Kit User Guide

Plant DNA Extraction Kit
Quick Start Guide

PD0050 Plant DNA Extraction Kit

1. Add up to 50mg of ground fresh or dried plant leaves to the lysis bead tube provided.

2. Add 600µl of Lysis Buffer, 60µl PPB and 5µl RNase.
   Mix for 3 mins using TissueLyser at max speed or vortex for 10 mins; then centrifuge at 20,000g for 2 mins.

3. Avoiding pellet, transfer up to 300–400µl of supernatant to clean centrifuge tube.
   Add 1000µl of Binding Buffer, vortex for 10–20s, and wait 5 mins.

4. Place tube on magnetic rack to capture.
   Wait 1 min then discard supernatant.

5. Add 600µl of Wash Buffer #1 to the tube and vortex for 10–20s.
   Wait 1 min then place tube on magnetic rack to capture. Wait 1 min then discard supernatant.

6. Add 600µl of Wash Buffer #2 to the tube and vortex for 10–20s. Place tube on magnetic rack to capture. Wait 1 min then discard supernatant.
   Repeat step 6 twice.
   **** Make sure to remove residual Wash #2 remaining in tube, or use drying step before elution.

7. Add 100µl of Elution Buffer to tube and mix briefly. Wait 1 min.
   Place tube on magnetic rack to capture.
   **** For increased yield heat Elution Buffer at 60°C for 5 mins.

8. Wait 1 min then transfer supernatant to clean microfuge tube..

Plant DNA Extraction Kit
PD0050-12
1.7

Documents / Resources

| Galenvs PD0050 Plant DNA Extraction Kit [pdf] User Guide
PD0050 Plant DNA Extraction Kit, PD0050, Plant DNA Extraction Kit, DNA Extraction Kit, Extraction Kit, Kit
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