GALENVS magnetic Plasmid Miniprep Kit User Guide
- June 3, 2024
- GALENVS
Table of Contents
Plasmid Miniprep Kit
User Guide
magnetic Plasmid Miniprep Kit
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Pellet 1–10ml of bacterial cell sample.
(<5ml for high-copy plasmid, <10ml for low-copy plasmid). Discard supernatant.
Add 200μl of Resuspension Buffer to the pellet and mix well by pipetting up- down until solution is homogenous.
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Add 200μl of Lysis Buffer and mix well by pipetting up-down (5–10x) until solution is homogenous.
Incubate for 8 mins at room temp.
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Add 100μl of Neutralization Buffer and mix well by pipetting up-down (5–10x).
Incubate for 2 mins at room temp.
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Place tube on magnetic rack for 1 min.
Transfer supernatant to a new microfuge tube. Discard previous tube. -
Add 400μl of Binding Buffer to the supernatant in the new microfuge tube and mix well by pipetting up-down (10-15x) until solution is homogenous.
Incubate for 1 min at room temp. -
Place microfuge tube on magnetic rack for 1 min. Discard supernatant.
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Add 600μl of Wash Buffer #1 and mix well by pipetting up-down (5–10x) until solution is homogenous.
Place microfuge tube on magnetic rack for 1 min. Discard supernatant.
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Repeat with 600μl of Wash Buffer #2, place on magnetic rack for 1 min.
Discard supernatant and leave to dry for 1–5 mins.
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Add 50–200μl of Elution Buffer and mix well by pipetting up-down (10–15x) until solution is homoegenous.
Incubate for 1 min.
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Place microfuge tube on magnetic rack for 1 min.
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Transfer pure plasmid supernatant to a clean microfuge tube.
PM Plasmid Miniprep Kit
PM0100-12
PM0250-12