GALENVS magnet Soil DNA Extraction Kit User Guide

GALENVS magnet Soil DNA Extraction Kit

Product Specifications

• Product Name: Soil DNA Extraction Kit
• Components: Lysis bead tube, Lysis Buffer, RNase, IR Buffer, Binding Buffer, Wash Buffer #1, Wash Buffer #2, Elution Buffer
• Maximum Soil Sample: Up to 250mg
• Centrifugation Speed: 20,000g

Product Usage Instructions

• Step 1: Sample Preparation
  • Add up to 250mg of soil sample to the lysis bead tube provided.
• Step 2: Lysis and Supernatant Collection
  • Add appropriate volumes of Lysis Buffer, RNase, and IR Buffer to the lysis bead tube.
  • Centrifuge the tube at 20,000g and collect the supernatant into a clean centrifuge tube.
• Step 3: Binding and Washing
  • Add Binding Buffer to the supernatant and mix well.
  • Perform centrifugation and wash steps using Wash Buffer #1 and Wash Buffer #2 as instructed in the manual.
  • Ensure complete removal of residual Wash #2 before proceeding to the next step.
• Step 4: Elution
  • Add Elution Buffer to the tube and incubate for 5 minutes. Then transfer the supernatant to a clean microfuge tube.

FAQs

• Q: How much soil sample can be processed with this kit?
  • A: The Soil DNA Extraction Kit can process up to 250mg of soil sample.
• Q: What is the recommended centrifugation speed?
  • A: The recommended centrifugation speed is 20,000g as specified in the manual.

USING INSTRUCTION

1. Add up to 250mg of soil sample to the lysis bead tube provided. Add 900µl of Lysis Buffer and 5µl of RNase, mix for 10 mins using TissueLyser at max speed, then centrifuge at 20,000g for 1 min.
2. Avoid the pellet, transfer up to 400–500µl of supernatant to clean the centrifuge tube. Add 200µl of IR buffer, vortex for 10–20s, then centrifuge for 1 min.
3. Avoid pellet, transfer up to 400–600µl of supernatant to clean the centrifuge tube. Add 1000µl of Binding Buffer, vortex for 10–20s, and wait 5 mins.
4. Place the tube on a magnetic rack to capture. Wait 1 min then discard the supernatant.
5. Add 600µl of Wash Buffer #1 to the tube and vortex for 10–20s. Wait 1 minute then place the tube on a magnetic rack to capture. Wait 1 minute then discard supernatant. Repeat this step.
6. Add 600µl of Wash Buffer #2 to the tube and vortex for 10–20s. Place the tube on a magnetic rack to capture. Wait 1 minute then discard the supernatant. Repeat this step. Make sure to remove residual Wash #2 remaining in a tube, or use the drying step before elution.
7. Add 100µl of Elution Buffer to the tube and mix briefly. Wait 1 minute. Place the tube on a magnetic rack to capture. For increased yield heat Elution Buffer at 60°C for 5 mins.
8. Wait 1 minute then transfer the supernatant to the clean microfuge tube.

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