Galenvs WW0016 Wastewater DNA RNA Extraction Kit User Guide
- May 15, 2024
- GALENVS
Table of Contents
Wastewater DNA/RNA
Extraction Kit
Quick Start Guide
WW0016 Wastewater DNA RNA Extraction Kit
Binding Buffer (600μl)
Columns 1 & 7
Binding magnetic nanoparticles (30μl)
Columns 2 & 8
Wash #1 Buffer (600μl)
Columns 3 & 9
Wash #2 Buffer (600μl)
Columns 4 & 10
Elution Buffer (100μl)
Columns 6 & 12
WWKit miQron protocol parameters
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Transfer 10ml of wastewater sample to 15ml conical tube. Add 100μl of Concentration Beads. Invert 5 times and incubate for 10 mins. Invert 3 more times at the 5 min mark .
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Place sample on 15ml magnetic rack to capture beads, then discard supernatant .
Remove tube from magnetic rack.
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Resuspend beads in 400μl of Lysis Buffer, then transfer to 2ml centrifuge tube.
Vortex at max speed for 5 mins, then separate beads using a 2ml magnetic rack.
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Remove the protective foil.
Avoiding pellet , transfer up to 400μl of supernatant to the Binding Buffer wells (columns 1 & 7 ).
You can add up to 16 samples. -
Place plate into the miQron, taking care that the label is facing outward.
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Insert two combs.
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Select the WWKit protocol and press
When program is complete, remove plate from miQron and discard combs.
Columns 6 & 12 contain the purified DNA/RNA elution.
**WW** Wastewater DNA/RNA Extraction Kit WW0016-12 1.3
Protocol Update
To import the updated protocol into the miQron, press the Scan Protocol icon from the Run menu (protocol list view window). Use the scanner on the QR code below.
Read User Manual Online (PDF format)
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