GALENVS WW0100 Wastewater DNA RNA Extraction Kit User Guide

GALENVS WW0100 Wastewater DNA RNA Extraction Kit

ASSEMBLY

1. Add 100µl of Concentration Beads (mix well before use) to 10ml wastewater sample, then invert 5 times to mix thoroughly. Incubate for 10 mins. At the 5-minute mark, invert 3 times to mix the beads.

2. Place the sample on a 15ml magnetic rack to capture beads, then discard the supernatant.
   Note: Store the beads at 4°C.

3. Add 400µl of Lysis Buer. Resuspend the beads by pipetting down. Transfer the mixture to a clean 2ml centrifuge tube. Mix for 5 mins using vortexer at maximum speed.

4. Use a 2ml magnetic rack to capture the beads. Avoiding the pellet, transfer up to 400µl of the supernatant into a clean 2ml microcentrifuge tube.

5. Add 600µl of Binding Buer. Add 30µl of Binding Beads. Vortex for 10-20s, and incubate for 5 mins. Place the tube on a magnetic rack to capture, wait 1 min then discard the supernatant.

6. Add 600µl of Wash Buer #1 to the tube and vortex for 10–20s. Wait 1 minute then place the tube on the magnetic rack to capture. Wait 1 minute then discard the supernatant.

7. Add 600µl of Wash Buffer #2 to the tube and vortex for 10–20s. Place the tube on a magnetic rack to capture. Wait 1 minute then discard the supernatant.

8. Add 100µl of Elution buffer to the tube and mix briefly. Incubate at 65°C for 10 mins. Place the tube on a magnetic rack to capture.

9. Wait 1 minute then transfer supernatant to a clean 2ml microcentrifuge tube.

Note
For increased yield perform elution twice with 50µl of buffer.

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