GALENVS magnet Plant RNA Extraction Kit User Guide

GALENVS magnet Plant RNA Extraction Kit

Product Information

Specifications:

• Product Name: Plant RNA Extraction Kit
• Protocol Versions: PRKIT-A PROTOCOL V1.0, PRKIT-B PROTOCOL V1.0
• Sample Capacity: Up to 16 samples
• Elution Volume: 50μL

Product Usage Instructions

Part A – RNA Extraction:

1. Add up to 50mg of fresh plant leaves sample to the lysis bead tube provided.
2. Mix for 10 mins using TissueLyser at max speed or vortex for 10 mins; then centrifuge at 20,000g for 2 mins.
3. Place the plate into the miQron, ensuring the label faces outward.
4. Insert two combs into the plate.
5. Select PRKit – Part A and run the protocol.
6. After completion, remove the plate from miQron and discard the combs.

Part B – DNase Treatment:

1. Mix DNase with buffer by gently inverting the tube a few times.
2. Add DNase Buffer to Elution Buffer (columns 6 & 12) without touching the walls.
3. Shake the Plate for 10 seconds and incubate at room temp for 20 mins.
4. Transfer 100μL of Elution Buffer to each well of the RNA Extraction Kit Plate.
5. Place the plate into the miQron, ensuring the label is outward.
6. Insert two combs and select PRKit – Part B to run the protocol.
7. Remove the plate from miQron and discard the combs after completion.

Frequently Asked Questions (FAQ)

• Q: How many samples can be processed in one extraction cycle?
  A: The kit can process up to 16 samples in a single extraction cycle.

• Q: What is the elution volume for purified RNA?
  A: The elution volume for purified RNA is 50μL, found in columns 5 and 11 of the kit plate.

Overview Description

PRKit–A miQron protocol parameters

Protocol Update

To import the updated protocol into the miQron, press the Scan Protocol icon from the Run menu (protocol list view window). Use the scanner on the QR codes below.

Part A RNA Extraction

Using Instruction

1. Add up to 50mg of fresh plant leaves sample to the lysis bead tube provided.

2. Add 600µl Lysis Buffer and 60µl PPB. Mix for 10 mins using TissueLyser at max speed or vortex for 10 mins; then centrifuge at 20,000g for 2 mins.

3. To the RNA Extraction Kit Plate A transfer up to 400µl of supernatant to Binding Buffer #1 (columns 1 & 7). You can add up to 16 samples.

4. Place the plate into the miQron, taking care that the label is facing outward.

5. Insert two combs.

6. Select PRKit – Part A and press
   When the program is complete, remove the plate from miQron and discard the combs.
   Proceed to Part B DNase Treatment
   Description
   PRKit-B miQron protocol parameters
   

7. Add 100µl of DNase Reaction Buffer to the DNase pellet.
   Mix by gently inverting the tube.
   The reconstituted pellet must be stored at -20 °C.

8. For each RNA sample, prepare DNase buffer by adding 10µl of DNase prepared in the previous step to 40µl of DNase Reaction buffer in a microfuge tube.
   Mix DNase with buffer by gently inverting the tube a few times.

9. To the RNA Extraction Kit Plate add 50µl of DNase buffer to Elution buffer (columns 6 & 12). Dispense directly into the elution buffer, not on the walls. If there are any droplets of elution buffer left on the walls, slide them to the well bottom by gently shaking or tapping the plate on a solid surface.

10. Gently shake the Plate A for 10 seconds by hand and incubate at room temp for 20 mins.
    From the RNA Extraction Kit Plate A transfer 100uL of the Elution Buffer (columns 6 & 12) to each well (columns 1 & 7) of the RNA Extraction Kit Plate B.

11. Place Plate B into the miQron, taking care that the label is facing outward.

12. Insert two combs.

13. Select PRKit – Part B and press When the program is complete, remove the plate from miQron and discard the combs.

Columns 5 and 11 contain the purified RNA elution.

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