GALENVS WW0016-12 Wastewater DNA and RNA Extraction Kit User Guide
- June 3, 2024
- GALENVS
Table of Contents
WW0016-12 Wastewater
DNA and RNA Extraction Kit
User Guide
WW0016-12 Wastewater DNA and RNA Extraction Kit
WWKit miQron protocol parameters
Step Name| Column| Volume
(μ l)| Time
(sec)| Mixing Speed
(1–10)| Dry Time
(sec)| Magnet Capture
Time (sec)| Temp
---|---|---|---|---|---|---|---
Load|
Bead Pickup| 2 & 8| 30| 60| Medium| 0| 1 x 30 sec| OFF
Binding| 1 & 7| 1000| 300| Medium| 0| 5 x 30 sec| OFF
Wash #1| 3 & 9| 600| 60| Medium| 0| 2 x 30 sec| OFF
Wash #2| 4 & 10| 600| 60| Medium| 120| 2 x 30 sec| OFF
Elution| 6 & 12| 100| 300| Low| 0| 2 x 30 sec| 65ºC
-Unload-| 2 & 8| | | | | |
-
Transfer 10ml of wastewater sample to 15ml conical tube. Add 100μl of Concentration Buffer to sample. Invert 5 times.
Add 75μl of Concentration Beads. Invert 5 times and incubate for 10 mins. Invert 3 more times at the 5 min mark. -
Place sample on 15ml magnetic rack to capture beads, then discard supernatant.
Remove tube from magnetic rack. -
Resuspend beads in 1ml RNase-free water, then transfer the mixture to a clean 2ml centrifuge tube.
Place tube on 2ml magnetic rack to capture beads, then discard supernatant. -
Remove tube from magnetic rack and resuspend beads in 400μl of Lysis Buffer.
Vortex at max speed for 5 mins, then separate beads using a 2ml magnetic rack. -
Avoiding pellet, transfer up to 400μl of supernatant to the Binding Buffer wells (Columns 1 & 7).
You can add up to 16 samples.
-
Place plate into the miQron, taking care that the label is facing outward.
-
Insert two combs.
-
Select the WWKit protocol and press When program is complete, remove plate from miQron and discard combs.
Columns 6 & 12 contains the purified DNA/RNA elution.
WW Wastewater DNA/RNA Extraction Kit|
WW0016-12
Documents / Resources
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GALENVS WW0016-12 Wastewater DNA and RNA Extraction
Kit
[pdf] User Guide
WW0016-12, Wastewater DNA and RNA Extraction Kit, WW0016-12 Wastewater DNA and
RNA Extraction Kit, DNA and RNA Extraction Kit, RNA Extraction Kit, Extraction
Kit
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