GALENVS QPM0200 Plasmid Miniprep Kit User Guide

Quick
Plasmid Miniprep Kit
Quick Start Guide

QPM0200 Plasmid Miniprep Kit

1. Add 4.4ml of GPL-1 solution to hydrate the GPL-2 tube containing lyophilized pellets.
   Ensure the prepared GPL-2 Buffer is mixed well by vortexing.
   Each GPL-2 tube provided contains 11 lyophilized pellets and is sufficient for 110 preps. The unused mixture should be kept at 4°C for storage and later use.

2. Pellet 1-10ml of bacterial cell sample.
   Resuspend bacterial pellet in 860pl of Lysis Buffer, then add 40ul of GPL-2 Buffer.
   Mix gently by pipetting up-down until solution is homogenous. Incubate for 5 mins at room temperature.
   For 10ml bacterial culture, add 80pl of GPL-2 Buffer for better ysis.

3. Add 40yl of Binding Magnetic Nanoparticles.
   Mix well by pipetting up-down (5-10x) until solution is homogeneous.
   Incubate for 2 mins at room temperature.

4. Place tube on magnetic rack to capture.
   Wait 1 min then discard supernatant.

5. Remove tube from magnetic rack and resuspend in 600l of Wash Buffer #1, and mix well by pipetting up-down (5-10x) until solution is homogeneous.
   Return to magnetic rack for 1-2 mins, then discard supernatant.

6. Repeat wash with 600l of Wash Buffer #2.
   Place tube on magnetic rack to capture.
   Wait 1 min then discard supernatant, and leave to dry for 2 mins.

7. Add 100yl of Elution Buffer to tube and mix well by pipetting up-down (10-15x) until solution is homogeneous.
   Incubate for 5 mins.
   Place tube on magnetic rack for 1 min.

8. Transfer pure plasmid DNA supernatant to a clean microfuge tube.

QPM
Quick Plasmid Miniprep Kit
QPM0200-12
1.4

Documents / Resources

| GALENVS QPM0200 Plasmid Miniprep Kit [pdf] User Guide
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