GALENVS UPX0100 Universal Pathogen Complex Module User Guide
- June 17, 2024
- GALENVS
Table of Contents
GALENVS UPX0100 Universal Pathogen Complex Module
Specifications:
- Product Name: Universal Pathogen Complex Matrix Module
- Sample Types: Milk, Fecal, oral fluid, viscous, swab, stool, exudate, urine, etc.
- Components: Pre-Treatment Beads (small and large), Proteinase K, Universal Pathogen DNA/RNA Extraction Kit
- Speed: 13,000 RPM
Product Usage Instructions
Step A: For Milk and Fecal Samples
- Ensure Pre-Treatment Beads (small) are used for milk samples and Pre-Treatment Beads (large) for fecal samples.
- Add the sample to the Universal Pathogen Complex Matrix Module.
- Include Proteinase K in the sample.
- Run the module at 13,000 RPM.
Step B: For Other Matrices
- Proceed with the Universal Pathogen DNA/RNA Extraction Kit.
- Add the sample to the module.
- Include Proteinase K in the sample.
- Operate the module at a speed of 13,000 RPM.
FAQ :
-
Q: Can I use the same kit for all sample types?
A: Yes, the Universal Pathogen Complex Matrix Module is designed to handle various sample types efficiently. Just follow the specific instructions for each type. -
Q: What is the purpose of Proteinase K in the process?
A: Proteinase K is used to digest proteins in the sample, facilitating the extraction of DNA/RNA for analysis. -
Q: Is there a difference between manual and automated operation?
A: The manual operation involves user intervention at different stages, while automated operation streamlines the process with predefined steps.
Universal Pathogen Complex Matrix Module
Quick Start Guide
- For milk and fecal samples, proceed to step A
- For all other matrices, eg. oral fluid, viscous, swab, stool, exudate, urine, etc., proceed to step B
Milk Fecal
- A To the 2mL Bead Beating tube provided, add 200µL of sample, 300µL Pre-Treatment Buffer (PTB) and 40µL Proteinase K. Shake well before use.
- Vortex for 30 seconds and heat at 70°C for 5 mins. To pellet debris and solids, briefly centrifuge at 13000rpm for 1 min.
- Note: use Pre-Treatment Beads (small) for milk and Pre-Treatment Beads (large) for fecal samples.
Other matrices
- B To a 1.5mL centrifuge tube, add 200µL of sample, 300µL Pre-Treatment Buffer (PTB) and 40µL Proteinase K. Shake well before use.
- Vortex for 30 seconds and heat at 70°C for 5 mins. To pellet debris and solids, briefly centrifuge at 13000rpm for 1 min.
Collect 300µL and proceed to
Universal Pathogen DNA/RNA Extraction Kit
- Manual: UP0050 UP0020
- Automated: UP0016 UP0096
UPX Universal Pathogen Complex Matrix Module
Read User Manual Online (PDF format)
Read User Manual Online (PDF format) >>