GALENVS UP0050 Universal Pathogen Extraction Kit User Guide

June 13, 2024
GALENVS

GALENVS UP0050 Universal Pathogen Extraction Kit

IMPORTANT INFORMATION

Before first use add ethanol to wash buffers as per label instructions.
Before extraction mix bottles well by inverting upside down several times.

USE INSTRUCTIONS

  1. In 1.5ml microfuge tube, add 100µl–200µl of sample (swabs, serum/plasma, whole blood, cerebrospinal fluid (CSF), stool, sputum, exudate, urine, or saliva).

  2. Add 600µl of Lysis/Binding Buffer Mix well by pipetting up-down 10–15x. Incubate for 5 mins.

  3. Add 30µl of binding magnetic nanoparticles. Mix well by pipetting up-down 10–15x.
    Incubate for 5 mins.

  4. Place tube on magnetic rack for 1–2 mins to capture DNA/RNA-bead complex, then discard supernatant.

  5. Remove tube from magnetic rack and resuspend DNA/RNA–bead complex in 600µl of Wash Buffer #1.
    Return to magnetic rack for 1–2 mins, then discard supernatant.

  6. Repeat wash with 600µl of Wash Buffer #2, return to magnetic rack for 1–2 mins, then discard supernatant and leave to dry for 1 min.

  7. Remove tube from magnetic rack and resuspend DNA/RNA–bead complex in 50–100µl of Elution Buffer.
    Mix well by pipetting up-down 15–20x to elute DNA/RNA from beads and let stand for 1–2 mins.
    Optional: elute at 60ºC for improved yield.

  8. Place tube on magnetic rack to separate beads (~1–2 mins). Transfer clean DNA/RNA solution (supernatant) to clean tube.

Universal Pathogen DNA/RNA Extraction Kit

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