GALENVS QPM0100 Plasmid Miniprep Kit User Guide
- June 13, 2024
- GALENVS
Table of Contents
**GALENVS QPM0100 Plasmid Miniprep Kit User Guide
**
Add 40µl of GPL-2 Buer to the GPL-1 Buer.
Ensure the GPL-1 Buer is mixed well by vortexing the tube a few times.
The unused mixture should be kept at 4°C for storage.
Pellet 1–2ml of bacterial cell sample.
Resuspend bacterial pellet in 360µl of Lysis
Buffer, then add 40µl of GPL-1 Buer.
Incubate for 5 mins at room temperature.
Add 40µl of Binding Magnetic Nanoparticles.
Mix well by pipetting up-down (5–10x) until
solution is homogenous.
Incubate for 2 mins at room temperature
Place tube on magnetic rack to capture.
Wait 1 min then discard supernatant.
Remove tube from magnetic rack and resuspend in 600µl of Wash Buffer #1, and mix well by pipetting up-down (5–10x) until solution is homogenous.
Return to magnetic rack for 1–2 mins, then discard supernatant.
Repeat wash with 600µl of Wash Buer #2.
Place tube on magnetic rack to capture.
Wait 1 min then discard supernatant, and leave to dry for 2 mins.
Add 100µl of Elution Buer to tube and mix well by pipetting up-down (10–15x) until solution is homogeneous.
Incubate for 5 mins.
Place tube on magnetic rack for 1 min.
Transfer pure plasmid supernatant to a clean microfuge tube.
1StepLysis Plasmid Miniprep Kit
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