GALENVS QSG BC v1.1 Blood & Cell DNA Extraction Kit User Guide

Blood & Cell DNA
Extraction Kit
Quick Start Guide

QSG BC v1.1 Blood & Cell DNA Extraction Kit

1. In 1.5m1 microfuge tube, add 20p1 of Proteinase K. Then add 100p1 of whole blood’ sample or 100p1 of cell suspension2 in lx PBS.
   1 For 200ul of whole blood, use 600u1 of Lysis/Binding Buffer in step
   2 Up to br106 cells in 10Ou1

2. Add 400p1 of Lysis/Binding Buffer and mix well by pipetting up-down 20x.
   Incubate 5 mins at room temp (20-25°C) to allow for lysis and DNA binding.

3. Place tube on magnetic rack for 5 mins to capture DNA-bead complex, then discard supernatant.

4. Remove tube from magnetic rack and resuspend DNA-bead complex in 600p1 of Wash Buffer #1. Mix well by pipetting up-down 10-15x.
   Return to magnetic rack for 5 mins, then discard supernatant.

5. Repeat wash with 600p1 of Wash Buffer #2, return to magnetic rack for 1-2 mins, then discard supernatant and leave to dry for Immo

6. Remove tube from magnetic rack and resuspend DNA-bead complex in 50-100p1 of Elution Buffer*. Mix well by pipetting up-down 15-20x to elute DNA from beads and let stand for 1-2 mins.

• Recommended elution is 50-1000 for blood sample and 100pl per 1×105 cell suspension sample

  7. Place tube on magnetic rack to capture beads (-1-2 mins).

  8. Transfer the eluted DNA solution (supernatant) to a clean microfuge tube.

BC Blood & Cell DNA Extraction Kit
BC0100-121
BCO250.121

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