GALENVS PD0016 Plant DNA Extraction Kit User Guide
- June 13, 2024
- GALENVS
Table of Contents
GALENVS PD0016 Plant DNA Extraction Kit
Product Information
The Plant DNA Extraction Kit is designed for extracting DNA from plant samples. It provides a quick and efficient method to isolate high-quality DNA for various downstream applications.
The kit includes the following components:
- Binding Buffer
- Lysis bead tube
- Lysis Buffer PPB
- RNase
- miQron plate
- Combs
Please note that the kit allows for the extraction of up to 16 samples simultaneously.
Product Usage Instructions
- Add up to 50mg of plant leaves sample to the lysis bead tube provided.
- For optimal results, perform one of the following methods:
- Use a TissueLyser at maximum speed and mix for 3 minutes.
- Vortex the sample for 10 minutes.
After mixing, centrifuge the tube at 20,000g for 2 minutes to pellet the cellular debris.
- Remove the protective foil from the miQron plate.
- Place the plate into the miQron, ensuring that the label is facing outward.
- Insert two combs into the designated slots on the miQron plate.
- Select the PDKit protocol on the miQron and press the “Run” button to start the extraction process.
- Once the program is complete, carefully remove the plate from the miQron and discard the combs.
- Columns 6 and 12 of the miQron plate contain the purified DNA elution.
Following these instructions will enable you to extract high-quality DNA from plant samples using the Plant DNA Extraction Kit.
PDKit miQron protocol parameters
How To Use
- Add up to 50mg of plant leaves sample to the lysis bead tube provided.
- Add 600µl Lysis Buffer, 60µl PPB, and 5µl RNase. Mix for 3 mins using TissueLyser at max speed or vortex for 10 mins; then centrifuge at 20,000g for 2 mins.
- Remove the protective foil.
- Avoiding pellets, transfer up to 400µl of supernatant to Binding Buffer (Columns 1 & 7). You can add up to 16 samples.
- Place plate into the miQron, taking care that the label is facing outward.
- Insert two combs.
- Select the PDKit protocol and press
When program is complete, remove plate from miQron and discard combs.
Columns 6 and 12 contain the purified DNA elution.
Plant DNA Extraction Kit.
Read User Manual Online (PDF format)
Read User Manual Online (PDF format) >>