GALENVS PR0050-12 Plant RNA Extraction Kit User Guide
- June 9, 2024
- GALENVS
Table of Contents
GALENVS PR0050-12 Plant RNA Extraction Kit
How To Use
- Add up to 50mg of ground fresh plant leaves to the lysis bead tube provided.
- Add 600µl of Lysis Buffer and 60µl PPB. Mix for 3 mins using TissueLyser at max speed or vortex for 10 mins; then centrifuge at 20,000g for 2 mins.
- Avoid pellet, transfer up to 300–400µl of supernatant to clean the centrifuge tube. Add 1000µl of Binding Buffer #1, vortex for 10–20s, and wait 5 mins.
- Place the tube on a magnetic rack to capture. Wait 1 min then discard the supernatant.
- Add 600µl of Wash Buffer #1 to the tube and vortex for 10–20s. Wait 1 min then place the tube on a magnetic rack to capture. Wait 1 min then discard the supernatant.
- Add 600µl of Wash Buffer #2 to the tube and vortex for 10–20s. Place the tube on a magnetic rack to capture. Wait 1 min then discard the supernatant.
- Add 100µl of Elution Buffer to the tube and mix briefly. Wait 1 min. Place the tube on a magnetic rack to capture.
- Wait 1 min then transfer the supernatant to the clean microfuge tube.
- Add 2.5 ml of DNase Reaction Buffer to the DNase pellet. Mix by gently inverting the tube.
- Add 50µl of DNase buffer to 50µl of RNA sample in a microfuge tube.
- Gently shake the mix for the 20s, and incubate at room temperature for 25 mins.
- Mix 40µl of Binding Beads with 400µl Binding Buffer #2.
- Add 400µl of Binding Bead and Binding Buffer #2 mixture to the microfuge tube and vortex for 10s. Incubate the mixture for 5 mins at room temperature.
- Place tube on a magnetic rack for 2 mins. Discard supernatant.
- Resuspend beads in 600µl Wash Buffer #3. Vortex for 10–20s. Place the tube on the magnetic rack, wait for 1 min, and discard the supernatant.
- Repeat the washing step with Wash Buffer #3.
- Dry beads on a magnetic rack for 2 mins after the third wash. Remove any wash buffer left at the bottom of the tube at the end of the drying step.
- Add 50µl of the Elution Buffer to the tube and mix briefly.
- Place the tube on the magnetic rack, wait for 1 min then transfer the supernatant to the clean tube.
Read User Manual Online (PDF format)
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