GALENVS magenti Universal Pathogen DNA/RNA Extraction Kit User Guide
- June 3, 2024
- GALENVS
Table of Contents
Universal Pathogen
DNA/RNA Extraction Kit
Quick Start Guide
magenti Universal Pathogen DNA/RNA Extraction Kit
Lysis / Binding Buffer (600µl)
Columns 1 & 7
Binding magnetic nanoparticles (200µl)
Columns 2 & 8
Wash #1 Buffer (600µl)
Columns 3 & 9
Wash #2 Buffer (600µl)
Columns 4 & 10
Elution Buffer (90µl)
Columns 6 & 12
200μl sample added to Lysis/ Binding Buffer| 90μl extracted and purified RNA
elution| 200μl sample added to Lysis/
Binding Buffer| 90μl extracted and purified RNA elution
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UPKit miQron protocol parameters
Step Name| Column| Volume
(μ1)| Time
(sec)| Mixing Speed
(1-10)| Dry Time
(sec)| Magnet Capture
Time (sec)
---|---|---|---|---|---|---
Lysis| 1 & 7| 800| 300| 7| 0| –
Bead Transfer| 2 & 8| 200| 30| 5| 0| 30
Binding| 1 & 7| 800| 300| 5| 0| 30
Wash #1| 3 & 9| 600| 60| 6| 0| 60
Wash #2| 4 & 10| 600| 60| 6| 60| 60
Elution| 6 & 12| 90| 120| 7| 0| 60
Discard Comb| 2 & 8| 600| –| 5| –| –
1. Remove the protective foil.| 2. Add up to 16 samples to Lysis/Binding
Buffer (columns 1 and 7).| 3. Place plate into the miQron, taking care that
the label is facing outward.
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| |
4. Insert two combs.| 5. Select the VRKit protocol and press| Columns 5 and
11 contain the purified RNA elution.
| When program is complete, remove plate from miQron and discard combs.|
UP Universal Pathogen DNA/RNA
Extraction Kit
VR0016-12
1.0
Documents / Resources
|
GALENVS magenti Universal Pathogen DNA/RNA Extraction
Kit
[pdf] User Guide
magenti Universal Pathogen DNA RNA Extraction Kit, magenti, Universal Pathogen
DNA RNA Extraction Kit, Pathogen DNA RNA Extraction Kit, Extraction Kit
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