GALENVS magenti Total RNA Extraction Kit User Guide
- June 3, 2024
- GALENVS
Table of Contents
Total RNA Extraction Kit
Quick Start Guide
Part A RNA Extraction
magenti Total RNA Extraction Kit
1 Spin down PAXgene® tube at 5000g for 10 mins. Discard supernatant.| 2
Resuspend in 1.8ml of nuclease-free, sterile water.
Transfer suspension to nuclease-free 2ml tube. Nuclease-free water
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3 Spin down tube at 5000g for 10 mins.
Discard supernatant.| 4 Resuspend pellet in 300µl of Suspension Buffer by
pipetting or vortexing.
Make sure pellet is fully resuspended in the buffer.
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5 To the Binding Buffer A plate, add 40µl Proteinase K to each sample well.
Transfer the 300µl resuspension into a well on the Binding Buffer A plate.
Dispense directly into the Binding Buffer, not on the walls.| 6 Place Binding
Buffer A plate into purification system with the Binding Magnetic
Nanoparticles A plate, three (3) Wash Buffer plates and one Elution Buffer
plate.
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7. Run the Total RNA PAXgene protocol.| 8 When the protocol completes, remove
the Elution Buffer plate for use in part
B: DNase treatment.
Discard the tip comb and other plates from the purification system.
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9 Prepare DNase buffer by adding one volume of DNase to 4 volumes of DNase
Buffer. Mix DNase with buffer by gently inverting the tube a few times.
Make sure DNase is perfectly mixed by observing disappearance of smears within
reaction buffer when inverting the tube.| 10 To the elution plate from part
add 50µl of DNase Buffer. Dispense directly into the elution buffer not on the
walls. If there are any droplets of elution buffer left on the walls, slide
them to the well bottom by gently shaking or tapping the plate on a solid
surface.
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11 Gently shake the elution plate for 10 seconds by hand and incubate at room
temperature for 20 min.| 12 Mix 4ml of Binding Magnetic Nanoparticles M
solution to 40ml of Binding Buffer B.
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13 Add 400µl of Binding Buffer B to each well of the Elution Buffer A
plate.| 14 Place the Elution plate from part A into purification system
purification system with two (2) Wash Buffer plates and one Elution Buffer
plate.
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15 Run the Total RNA DNase CleanUp Protocol.| 16 When the protocol complete,
remove the final Elution Buffer plate containing the purified RNA.
Discard the tip comb and remaining plates from the purification system.
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TRB Total RNA Extraction Kit
TRB 0096-121.0
Documents / Resources
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GALENVS magenti Total RNA Extraction
Kit
[pdf] User Guide
magenti Total RNA Extraction Kit, magenti, Total RNA Extraction Kit, RNA
Extraction Kit, Extraction Kit
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Read User Manual Online (PDF format)
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