Levenhuk Discovery Atto microscopes User Manual

: June 5, 2024
: levenhuk

Table of Contents

Product Overview
General use
Microscope assembly
Using the microscope
Specifications
Care and maintenance
Battery safety instructions
Levenhuk Warranty
Levenhuk prepared slides sets
References
Read User Manual Online (PDF format)
Download This Manual (PDF format)

Levenhuk Discovery Atto microscopes

Product Overview

Eyepiece
Monocular head (eyepiece tube)
Revolving nosepiece with objectives
Coarse focusing knob
Fine focusing knob
Mechanical scale
Stage
Stage moving knob (right/left and back/forth)
Condenser with filter holder
Aperture diaphragm adjustment rod
Lower illumination
Upper illumination
Base
Carrying handle
Illumination on/off button
Power connector
Brightness adjustment knob

General use

The Discovery Atto microscopes are safe for health, life and property of the consumer and the environment when properly used, and meet the requirements of international standards. The microscopes are designed for observing transparent and opaque objects in the transmitted and reflected light using the bright field method.

They perform well in clinical research and tests; teaching demonstrations; bacterioscopy and cytology in medical and health establishments, laboratories, universities; and may be used for scientific research in agriculture and microbiology.

Caution! Children should use the microscope under an adult’s supervision only.

Microscope assembly

Unpack the microscope and make sure all parts are available.
Move the stage to the lowermost position using the focusing knob.
Make sure the batteries are correctly installed in the battery compartment; insert new batteries if required.
You can also plug the power cord into the microscope and then plug it in to the power source.
Insert the eyepiece into the eyepiece tube.
For the digital camera model: you can attach the digital camera to the eyepiece tube instead of the eyepiece.

Using the microscope

Turn on the power and the illumination will turn on. Set the brightness at approximately 70%.
Place the specimen on the stage. Make sure the clips hold the specimen firmly in place.
Iris diaphragm should be adjusted in accordance with numerical aperture of a lens.
The best practice is to make the iris diaphragm slightly smaller than the aperture of the selected lens. Open or close the iris diaphragm using the adjustment screws. If the adjustment screw is turned to the right, the iris diaphragm is fully open.
Note: the diaphragm is not intended for adjusting the brightness of the illumination. To adjust the brightness, use the brightness adjustment knob.
Slide the filter holder, place the filter in it and then return the holder to its initial position.
To change the objective magnification, turn the revolving nosepiece until it clicks.
Make sure that the lens does not touch the sample when adjusting the focus: rotate the coarse focus knob until the specimen is about 3.175mm (1/8″) away from the lens.
Looking into the eyepiece, slowly turn the coarse focusing knob until you can see the image of the observed specimen. Turn the fine focusing knob to make the image clear. The fine focusing mechanism allows you to focus on the observed specimen while using high magnifications.
When you use an oil immersion objective, the space between the specimen and the lens should be filled with immersion oil. Raise the condenser to the upper position and put a drop of oil on the lens and on the cover glass of the specimen. Move the revolving nosepiece back and forth to get rid of air bubbles in the oil. Then, fix the objective lens in its working position. Make sure that oil fills the entire space between the objective lens and the specimen. After use, wipe the lens dry.
To place the specimen in the field of view, move the stage horizontally back and forth or left and right using the stage moving knob.

Selecting the objective
Start your observations with the lowest magnification objective and select a specimen segment for detailed research. Then move the specimen to center the selected segment in the field of view, to make sure it keeps centered when the objective is changed to a more powerful one. Once the segment is selected, you should center its image in the microscope’s field of view as precisely as possible. Otherwise, the desired segment might fail to center in the field of view of the higher power objective. Now, you can switch to a more powerful objective by rotating the revolving nosepiece. Adjust the image focus if required.

Digital camera (for the digital model only)
The digital camera is installed in the eyepiece tube in place of the eyepiece. It allows to observe specimens in fine detail and true colors on your PC monitor and save images on the hard drive. The software allows viewing and editing the object images.

Megapixels	5
Max. resolution (for still images), pixels	2592×1944
Sensor	1/2.5″ CMOS
Pixel size, μm	2.2×2.2
Frame rate, fps	2@2592×1944

3@2048×1536
5@1600×1200
7.5@1280×1024
Video recording| +
Image format| .jpg, .bmp, .png, .tif
Video format| .wmv, .avi
Exposure| ERS
Interface| USB 2.0, 480Mbit/s
System requirements| Windows XP (32-bit), Vista/7/8/10/11 (32-bit or 64-bit), Mac OS X, Linux, CPU IntelCore 2 or above, RAM 2GB, USB port 2.0, CD-ROM

Specifications

| Biological model| Digital model
---|---|---
Type| biological| biological, digital
Magnification, x| 40—1000| 40—1000
Head| monocular, 360° rotatable, inclined 45°| monocular, 360° rotatable, inclined 45°
Optics material| optical glass| optical glass
Body material| metal| metal
Eyepiece tube diameter, mm| 23.2| 23.2
Eyepieces| WF10x with pointer| WF10x with pointer
Revolving nosepiece| 4 objectives| 4 objectives
Objectives| achromatic: 4x, 10x, 40xs, 100xs (oil)| achromatic: 4x, 10x, 40xs, 100xs (oil)
Stage, mm| 105×95, double layer mechanical, with a mechanical scale| 105×95, double layer mechanical, with a mechanical scale
Stage moving range, mm| 75/20| 75/20
Focusing| coaxial, coarse (0.5mm) and fine (0.002mm)| coaxial, coarse (0.5mm) and fine (0.002mm)
Condenser| Abbe N.A. 1.25 iris diaphragm and filter holder| Abbe N.A. 1.25 iris diaphragm and filter holder
Illumination| LED; upper and lower illumination; with brightness adjustment| LED; upper and lower illumination; with brightness adjustment
Vial of immersion oil| +| +
Power source| 3АА batteries or 110—240V, via AC adapter| 3АА batteries or 110—240V, via AC adapter
Digital camera| —| 5 megapixels

The manufacturer reserves the right to make changes to the product range and specifications without prior notice.
Note: Batteries might be pre-installed in the battery compartment by the manufacturer.

Care and maintenance

Never, under any circumstances, look directly at the Sun, another bright source of light or at a laser through this device, as this may cause PERMANENT RETINAL DAMAGE and may lead to BLINDNESS.
Take necessary precautions when using the device with children or others who have not read or who do not fully understand these instructions.
After unpacking your microscope and before using it for the first time check for integrity and durability of every component and connection.
Do not try to disassemble the device on your own for any reason. For repairs and cleaning of any kind, please contact your local specialized service center.
Protect the device from sudden impact and excessive mechanical force. Do not apply excessive pressure when adjusting focus. Do not overtighten the locking screws.
Do not touch the optical surfaces with your fingers. To clean the device exterior, use only special cleaning wipes and special optics cleaning tools from Levenhuk. Do not use any corrosive or acetone-based fluids to clean the optics.
Abrasive particles, such as sand, should not be wiped off lenses, but instead blown off or brushed away with a soft brush.
Do not use the device for lengthy periods of time or leave it unattended in direct sunlight. Keep the device away from water and high humidity.
Be careful during your observations, always replace the dust cover after you are finished with observations to protect the device from dust and stains.
If you are not using your microscope for extended periods of time, store the objective lenses and eyepieces separately from the microscope.
Store the device in a dry, cool place away from hazardous acids and other chemicals, away from heaters, open fire, and other sources of high temperatures.
When using the microscope, try not to use it near flammable materials or substances (benzene, paper, cardboard, plastic, etc.), as the base may heat up during use, and might become a fire hazard.
Always unplug the microscope from a power source before opening the base or changing the illumination lamp. Regardless of the lamp type (halogen or incandescent), give it some time to cool down before trying to change it, and always change it to a lamp of the same type.
Always use the power supply with the proper voltage, i.e. indicated in the specifications of your new microscope. Plugging the instrument into a different power outlet may damage the electric circuitry of the microscope, burn out the lamp, or even cause a short circuit.
Seek medical advice immediately if a small part or a battery is swallowed.

Battery safety instructions

Always purchase the correct size and grade of battery most suitable for the intended use.
Always replace the whole set of batteries at one time; taking care not to mix old and new ones, or batteries of different types.
Clean the battery contacts and also those of the device prior to battery installation.
Make sure the batteries are installed correctly with regard to polarity (+ and –).
Remove batteries from equipment that is not to be used for an extended period of time.
Remove used batteries promptly.
Never short-circuit batteries as this may lead to high temperatures, leakage, or explosion.
Never heat batteries in order to revive them.
Do not disassemble batteries.
Remember to switch off devices after use.
Keep batteries out of the reach of children, to avoid risk of ingestion, suffocation, or poisoning.
Utilize used batteries as prescribed by your country’s laws.

Levenhuk Warranty

Levenhuk products, except for their accessories, carry a 2-year warranty against defects in materials and workmanship. All Levenhuk accessories are warranted to be free of defects in materials and workmanship for six months from the purchase date. The warranty entitles you to the free repair or replacement of the Levenhuk product in any country where a Levenhuk office is located if all the warranty conditions are met.
For further details, please visit: www.levenhuk.com/warranty
If warranty problems arise or if you need assistance in using your product, please contact the local Levenhuk branch.

Levenhuk prepared slides sets

Levenhuk Prepared Slides Sets

The Levenhuk slides sets include thoroughly prepared specimens of various biomaterials as well as blank slides and cover glasses for you to make your own specimens. Prepared slides, blank slides and cover slips can be used with any microscope model.

Compact but powerful portable telescopes with coated optics, smartphone adapter, backpack, and an amazing knowledge book “Space. Non-Empty Emptiness”

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