Levenhuk 500B Binocular Microscope User Manual

: June 1, 2024
: levenhuk

Table of Contents

500B Binocular Microscope
General Information
Microscope assembly
Specification
Care and maintenance
Levenhuk prepared slides sets
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Download This Manual (PDF format)

Levenhuk 500 Series
Microscopes
Levenhuk 500M
Levenhuk 500B
Levenhuk 500T
Levenhuk 500T POL
User Manual

Levenhuk 500B Binocular MicroscopeE Zoom ran and hab Fun!

500B Binocular Microscope

1a. Levenhuk 500M 1b. Levenhuk 500B 1c. Levenhuk 500T 1d. Levenhuk 500T POL

Eyepiece
Eyepiece head
Monocular/binocular/trinocular head locking screw
Arm
Revolving nosepiece
Objective
Specimen holders
Mechanical scale
Stage
Aperture diaphragm adjustment rod
Condenser with filter holder
Coarse focusing knob
Fine focusing knob
Illumination on/off button (not shown)
Collector
Brightness adjustment knob
Diopter adjustment ring
Stage moving knob (right/left)
Stage moving knob (back/forth)
Third eyepiece tube (trinocular models only)
Polarizer (500T POL only)
Analyzer slot (not shown)
Stage locking screw (500T POL only)

2a. Levenhuk 500T POL 2b. Levenhuk 500T POL

General Information

Read the user manual carefully before you start working with a microscope. Levenhuk 500 Series biological microscopes are safe for health, life and property of the consumer and the environment when properly used, and meets the requirements of international standards. These microscopes are designed for observing transparent objects in the transmitted light using the bright field method. They perform well in clinical research and tests; teaching demonstrations; bacterioscopy and cytology in medical and health establishments, laboratories, universities; and may be used for scientific research in agriculture and microbiology.

Microscope assembly

Unpack the microscope carefully and place it on a flat surface.
Remove the plastic bags and dustproof cover of the eyepiece head.
Install the monocular, binocular or trinocular head and tighten the locking screw.
Inspect all the items included in the kit and define their purpose.
Connect the power cord to the microscope and plug it in.

Use

Please refer to the fig. 1a (monocular model), fig. 1b (binocular model), fig. 1¢ and 1d (trinocular model).

Turn on the power and the illumination will turn on. Set the brightness at approximately 70%. Place the specimen on the stage. Make sure the clips hold the specimen firmly in place.
Iris diaphragm should be adjusted in accordance with numerical aperture of a lens. The best practice is to make the iris diaphragm slightly smaller than the aperture of the selected lens. Open or close the iris diaphragm using the adjustment screws. If the adjustment screw is turned to the right, the iris diaphragm is fully open. Note: the diaphragm is not intended for adjusting the brightness of the illumination. To adjust the brightness, use the brightness adjustment knob.
Slide the filter holder, place the filter in it and then return the holder to its initial position.
To change the objective magnification, turn the revolving nosepiece until it clicks.
Make sure that the lens does not touch the sample when adjusting the focus: rotate the coarse focus knob until the specimen is about 3.175mm (1/8″) away from the lens.
Looking into the eyepiece, slowly turn the coarse focusing knob until you can see the image of the observed specimen. Turn the fine focusing knob to make the image clear. The fine focusing mechanism allows you to focus on the observed specimen while using high magnifications.
When you use an oil immersion objective, the space between the specimen and the lens should be filled with immersion oil.
Raise the condenser to the upper position and put a drop of oil on the lens and on the cover glass of the specimen. Move the revolving nosepiece back and forth to get rid of air bubbles in the oil. Then, fix the objective lens in its working position.
Make sure that oil fills the entire space between the objective lens and the specimen. After use, wipe the lens dry.
To adjust the position of the mechanical scale (except for SOOM and 500T POL), turn the coarse focusing knob in a clockwise direction if you want to fix the position of the mechanical scale, or in a counterclockwise direction if you want to loosen it.
To place the specimen in the field of view, move the stage horizontally back and forth or left and right using the stage moving knobs (except for 500M and SOOT POL).
Look through the right eyepiece with your right eye and adjust the image sharpness with the coarse and fine focusing knobs.
Binocular and trinocular models: Then, look through the left eyepiece with your left eye and rotate the diopter adjustment ring to equalize the difference between your left and right eyes vision. Remember the setting for future use. Adjust the distance between the eyepiece tubes of the binocular head so that the image merges into a single circle.
Trinocular models: you can install the digital camera to the third eyepiece tube.

Observation in polarized light
The Levenhuk 500T POL microscope is equipped with an analyzer (Fig. 2a) and is equipped with an integrated polarizer (Fig. 2b).
They allow you to reduce glare and spurious reflections when studying shiny objects. Insert the analyzer into a special slot on the back of the microscope to start observing in polarized light. Set the polarizer to “0” — the polarizer and analyzer will be positioned orthogonally. Rotate the polarizer ring to change the polarization angle. The Levenhuk 500T POL microscope is equipped with a round stage. By using the rotation of the stage, you can change the angle of polarization and observe changes in the refraction of light. Loosen the retainer in order to rotate the stage and tighten the retainer to secure the stage.
Halogen bulb replacement
Unplug the microscope from a power source. Loosen the knurled screw on the underside of microscope and open the panel to expose the bulb. Make sure the lamp has cooled down and then remove it. Do not touch the new lamp with your fingers; fingerprints or dirt will reduce the brightness and the lamp’s life. Use gloves to replace the lamp. If necessary, wipe the lamp with a clean, soft cloth. Use a lamp of a suitable type. Insert the contacts into the slot vertically. Close the panel and tighten the knurled screw with finger.
Fuse replacement
Unplug the microscope from a power source. Open the fuse holder located on the back of the microscope body with a flat head screwdriver. Remove the old fuse and install a new one. Only use the appropriate types of fuses. Close the fuse holder.

Specification

Product ID	75424	75425	75426	75427
Model	Levenhuk 500M	Levenhuk 5006	Levenhuk 500T

Levenhuk reserves the right to modify or discontinue any product without prior notice.
Caution: Please refer to the specifications table for the correct mains voltage and never attempt to plug a 110V device A into 220V outlet and vice versa without using a converter. Remember that mains voltage in the U.S. and Canada is 110V and 220—240V in most European countries.

Care and maintenance

Never, under any circumstances, look directly at the Sun, another bright source of light or at a laser through this device, as this may cause PERMANENT RETINAL DAMAGE and may lead to BLINDNESS.
Take necessary precautions when using the device with children or others who have not read or who do not fully understand these instructions.
After unpacking your microscope and before using it for the first time check for integrity and durability of every component and connection.
Do not try to disassemble the device on your own for any reason. For repairs and cleaning of any kind, please contact your local specialized service center.
Protect the device from sudden impact and excessive mechanical force. Do not apply excessive pressure when adjusting focus. Do not overtighten the locking screws.
Do not touch the optical surfaces with your fingers. To clean the device exterior, use only special cleaning wipes and special optics cleaning tools from Levenhuk. Do not use any corrosive or acetone-based fluids to clean the optics.
Abrasive particles, such as sand, should not be wiped off lenses, but instead blown off or brushed away with a soft brush.
Do not use the device for lengthy periods of time, or leave it unattended in direct sunlight. Keep the device away from water and high humidity.
Be careful during your observations, always replace the dust cover after you are finished with observations to protect the device from dust and stains.
If you are not using your microscope for extended periods of time, store the objective lenses and eyepieces separately from the microscope.
Store the device in a dry, cool place away from hazardous acids and other chemicals, away from heaters, open fire and other sources of high temperatures.
When using the microscope, try not to use it near flammable materials or substances (benzene, paper, cardboard, plastic, etc.), as the base may heat up during use, and might become a fire hazard.
Always unplug the microscope from a power source before opening the base or changing the illumination lamp. Regardless of the lamp type (halogen or incandescent), give it some time to cool down before trying to change it, and always change it to a lamp of the same type.
Always use the power supply with the proper voltage, i.e. indicated in the specifications of your new microscope. Plugging the instrument into a different power outlet may damage the electric circuitry of the microscope, burn out the lamp, or even cause a short circuit.
Seek medical advice immediately if a small part or a battery is swallowed.

Levenhuk International Lifetime Warranty

All Levenhuk telescopes, microscopes, binoculars and other optical products, except for accessories, carry a lifetime warranty against defects in materials and workmanship. Lifetime warranty is a guarantee on the lifetime of the product on the market.
All Levenhuk accessories are warranted to be free of defects in materials and workmanship for six months from date of retail purchase. The warranty entitles you to free repair or replacement of the Levenhuk product in any country where a Levenhuk office is located if all warranty conditions are met.
For further details please visit our web site: www.levenhuk.com/warranty
If warranty problems arise, or if you need assistance in using your product, contact the local Levenhuk branch.

Levenhuk prepared slides sets

The Levenhuk slides sets include thoroughly prepared specimens of various biomaterials as well as blank slides and cover glasses for you to make your own specimens. Prepared slides, blank slides and cover slips can be used with any microscope model.

www.levenhuk.com
Levenhuk Inc. (USA): 928 E 124th Ave.
Ste D, Tampa, FL 33612,
USA, +1-813-468-3001,
contact_us@levenhuk.com
Levenhuk Optics s.r.o. (Europe):
V Chotejné 700/7, 102 00 Prague 102,
Czech Republic, +420 737-004-919,
sales-info@levenhuk.cz
Levenhuk® is a registered
trademark of Levenhuk, Inc.
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All rights reserved.
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