biosan TS-100C Thermo-Shaker for Microtubes and PCR Plates Instruction Manual

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TS-100 & TS-100C
Thermo-shakers for microtubes and PCR plates

User Instructions

About this edition of the user instructions

1.1. The current edition of the user instructions applies to following models:

• TS-100 version V.6AW
• TS-100C version V.7AW

 Safety precautions

Caution! Make sure you have fully read and understood the present user instructions before using the equipment. Please pay special attention to sections marked by this symbol.
Caution! Hot surface! Platform surface becomes very hot during use. Always use protective cotton gloves to install or remove samples when the temperature is set higher than 60°C.

GENERAL SAFETY

• The protection provided can be ineffective if the operation of the appliance does not comply with the manufacturer’s requirements.
• Save the unit from shocks or falling.
• Store and transport the unit in a horizontal position (see package label) at ambient temperatures between -20°C and +60°C and maximum relative humidity of 80%.
• After transportation or storage keep the unit under room temperature for 2-3 h before connecting it to the electric circuit.
• Use only original parts and accessories, provided by manufacturer for this product.
• Before using any cleaning or decontamination methods except those recommended by the manufacturer, check with the manufacturer that the proposed method will not damage the equipment.
• Do not make modifications to the design of the unit.

ELECTRICAL SAFETY

• Connect only to the external power supply with voltage corresponding to that on the serial number label.
• Use only the external power supply provided with this product.
• Ensure that the power switch and external power supply are easily accessible during use.
• Do not plug the unit into an ungrounded power socket, and do not use an ungrounded extension lead.
• Disconnect the unit from electric circuit before moving.
• If liquid penetrates into the unit, disconnect it from the external power supply and have it checked by a repair and maintenance technician.
• Do not operate the unit in premises where condensation can form. Operating conditions of the unit are defined in the Specifications section.

DURING OPERATION

• Do not leave the operating unit unattended.

• Do not impede the platform motion.

• Do not operate the unit in environments with aggressive or explosive chemical mixtures.
  Please contact manufacturer for possible operation of the unit in specific atmospheres or with dangerous materials.

• Do not operate the unit if it is faulty or has been installed incorrectly.

• Do not use outside laboratory rooms.

• Do not check the temperature by touch. Use a thermometer.

ALARM SOUND SIGNALS

• Frequently repeating short notes after finishing the operation (see 5.6.3).
• Infrequently repeating short notes if an error occurred (see 9.5)

BIOLOGICAL SAFETY

• It is the user’s responsibility to carry out appropriate decontamination if hazardous material is spilt on or penetrates into the equipment.

General information

TS-100 and TS-100С thermo-shakers are designed for intensive mixing of samples in microtest tubes or PCR plates in a temperature-controlled environment. The TS-100C
model of thermo-shaker differs from TS-100 in the possibility of cooling samples down to +4°C. Features of thermo-shakers meet the highest expectations of users according to many parameters:

• Fast reaching of specified mixing speed and maintenance of equal amplitude of rotation throughout the thermo-shaker block;

• Stability of maintaining the set temperature in a wide range throughout the block surface of thermo-shakers;

• With the help of the temperature calibration function, the user can calibrate the unit approximately ±6% of the selected temperature to compensate differences in the
  thermal behaviour of tubes from different manufacturers;

• LCD display indicates pre-set and current values of temperature, speed and time of operation;

• Quiet motor operation, compact size, prolonged service life;

• Sensor error handling and diagnostics;

Functions of heating and mixing can be performed either simultaneously or inde- pendently, that allows using the unit as three independent devices:

1. Thermostat;
2. Shaker;
3. Thermo-shaker.

We offer five heating and cooling blocks for each model, including a block with a plastic lid for PCR-plates. Within one model of thermo-shaker, the blocks are mutually interchangeable and can be easily  installed.
The devices are applicable in:

• genetic analyses – in extraction of DNA, RNA and further sample preparation;
• biochemistry – for studying of enzymatic reactions and processes;
• cellular biology – extraction of metabolites from cellular material.

 Getting started

4.1. Unpacking. Remove packing materials carefully and retain for future shipment or storage of the unit. Examine the unit carefully for any damage incurred during transit.
The warranty does not cover in-transit damage. Warranty covers only units transported in the original package.
4.2. Complete sets.
4.2.1. TS-100
– TS-100 thermo-shaker for microtubes and microplates ……………….. 1 pce
– External power supply …………………………….. 1 pce
– Power cable …………………………………………………. 1 pce
– Spare rubber belt …………………………… 2 pcs
– Operating manual, declaration of conformity ……………………… 1 pce
– SC-18 thermoblock for microtubes ………………….. on request
– SC-18/02 thermoblock for microtubes ……………………………. on request
– SC-24 thermoblock for microtubes ………………………… on request
– SC-24N thermoblock for microtubes ……………………………. on request
– SC-96A thermoblock for microplate and hex-key ………………. on request

4.2.2. TS-100C
– TS-100C thermo-shaker with cooling for microtubes and microplates ………….. 1 pce
– External power supply ………………………………………… 1 pce
– Power cable …………………………………………….. 1 pce
– Spare rubber belt ………………………………….. 2 pcs
– Operating manual, declaration of conformity ……………………………….. 1 pce
– SC-18C thermoblock for microtubes ……………………………… on request
– SC-18/02C thermoblock for microtubes …………………………. on request
– SC-24C thermoblock for microtubes …………………………….. on request
– SC-24NC thermoblock for microtubes ………………………… on request
– SC-96AC thermoblock for microplate and hex-key …………………. on request

4.3. Setup.
– Place the unit upon even horizontal stable non-flammable surface 30 cm away from any flammable materials, and clear 20 cm around the device on all sides for ventilation.
– Remove protective film from the display;
– Plug the external power supply into the socket at the rear side of the unit;
– Connect the power cable to the external power supply.
4.4. Thermoblock installation (if a thermoblock is not installed).
Caution! Thermoblock installation and replacement have to be performed only when the Power switch is turned off and external power supply is disconnect from the device.
Caution! Thermoblocks for TS-100 and TS-100C are not interchangeable! Installing a thermoblock from different model will irreversibly damage both the unit and the thermoblock! TS-100C thermoblocks have an additional sticker.

 – Choose the thermoblock, connect the plug to the contact terminal according to the scheme on fig. 1/1 on the underside of the thermoblock. Make sure that the connector is mounted tightly.
– Align the thermoblock so that the warning label ****is facing the front of the unit (fig. 2).
– Secure with the four knurled screws (fig. 2/1) or four hex screws.
4.5. Changing blocks.
– Disconnect the external power supply from the device.
– Remove the four knurled screws or four hex screws (in microplate thermoblocks).
– Lift the block without damaging the cable and disconnect the plug (fig. 1/1).
– Select the new thermoblock and install it according to 4.4.

Operation

Recommendations during operation

• Please check the tubes/microplates before using, be sure that tubes and micro plates are heat-resistant. Do not heat the microplates over the melting point of the material they are made of.
• We recommend filling tubes and plate wells up to 75% of rated volume for efficiency.
  Caution! Platform surface becomes very hot during use. Please, take necessary care and use protective cotton gloves to install or remove test samples when set temperature is higher than 60°C.

5.1. Connect external power supply to a grounded power socket and set the power switch, located on the rear panel of the unit, to position I (ON).
5.2. The display will turn on with the upper line (Set) showing time, speed and temperature set earlier and the lower line (Actual) showing current status: STOP indication, 000 rpm speed and platform temperature in °C.
5.3. If a temperature is set, then the platform temperature automatically changes to that temperature. The time of temperature stabilization depends on the room temperature.
If the heating of is turned off by setting the temperature below 25°C (TS-100) or 4°C (TS-100C), top line shows indication OFF.
5.4. Setting the parameters. Use the readings in the upper line of the display (Set), while setting the required parameters. Pressing the key for more than 3 s will increase the
increment rate. Speed and temperature can be changed during operation.
5.4.1. Setting time (TIME). Using the and TIME keys (fig. 3/1) set the required working time interval in hours and minutes (increment 1 min).
5.4.2. Setting speed (RPM). Using the and RPM keys (fig. 3/2) set the required speed (increment 10 rpm).
5.4.3. Setting temperature (Т, ºС). Using the and Т, ºС keys (Fig. 3/3) set the necessary temperature (increment 0.1°C).
Caution! Heating/temperature maintenance process does not stop when the timer is finished. Platform thermal regulation can be turned off only by setting the required temperature below 25°C (TS-100) or 4°C (TS100C), top line shows indication OFF. In this mode, thermo-shaker can be used in the cold rooms as a mixing device without thermoregulation.
5.5. Program execution. After the thermal stabilization of the thermo-shaker, i.e. when the set and current temperature readings become the same:
5.5.1. Place samples on the platform.
Caution!
Do not fill microtubes or microplates directly inside the unit.
5.5.2. Press the ►/■ RUN/STOP key (fig. 3/4). The platform will start rotating and the timer indicator will start counting up the time interval (with 1 min precision).
Note! If the rotation speed is set to zero, pressing ►/■ RUN/STOP key will start the timer but the platform will not move.
5.5.3. After finishing the program (after the set time elapses) the platform motion will stop and the timer will show the flashing reading STOP accompanied by the repetitive
sound signal until the ►/■ RUN/STOP key is pressed.
5.6. If the working time is not set (or is reset) and the timer indicator in the upper line shows 00:00, pressing the ►/■ RUN/STOP key will start continuous operation of the
device with countdown timer in the lower line (Actual) until the ►/■ RUN/STOP key is pressed again.
5.7. If required, there is possibility to restart the timer when it is running. Press the TIME RUN/STOP key once (fig. 3/5) to stop the timer. Press the TIME RUN/STOP key again to restart the timer.
5.8. The platform motion can be stopped at any time by pressing the ►/■ RUN/STOP key. In this case the program realization and the platform motion will stop and the
timer will switch into the STOP mode saving previously set time. Press the ►/■ RUN/STOP key to repeat the operation with the same time and speed.
Caution! At the end of the set time period the platform movement is stopped automatically, but the heating can be stopped only manually by reducing the temperature using the   T, °C key (Fig. 3/3 – lower key) till the OFF sign appears in the upper line (Set) of the display
Caution! The platform remains hot after use. Please, take necessary care and use protective cotton gloves to install or remove test samples when set temperature is higher than 60°C.
5.9. After finishing the operation, set the Power switch, located on the rear panel of the unit, in position O (Off) and disconnect the external power supply from electric circuit.

Calibration

6.1. The device is precalibrated at the factory (calibrating coefficient is 1.000) for operation with temperatures measured by a sensor in the heating block.
6.2. To change the calibration coefficient, hold the TIME RUN/STOP key pressed for more than 8 s to activate calibration mode. The calibration coefficient appears on the display (figure 4).
Figure 4. Display in calibration mode:

1. Calibration mode indicator;
   

2. Calibration coefficient;

3. Temperature with current coefficient

Note. Values marked in grey on figures 4 and 5 are not used in calibration and are meant for service engineers.
6.3. Restoring factory settings. Set 1.000 value using the ▲ and ▼ T, °C keys as shown on fig. 4/1 to restore the factory settings. Press the ►/■ RUN/STOP key once to save the changes and exit the calibration mode.
Note.Coefficient value changes are recommended after the unit has reached 30°C temperature.
6.4. Calibration procedure. To calibrate the unit, use an independent sensor with 0.5°C accuracy, which can fit in the cell of a microplate on the platform.
6.4.1. Install the sensor into a celll of the microplate.
6.4.2. Set the required temperature in operation mode (e.g. 40°C).
6.4.3. After the unit reaches the set temperature (when the set and current temperature readings equal), leave the unit for 30 min for thermal stabilization.
6.4.4. Let us assume that the readings of independent sensor is 39°C, but the display’s actual temperature is 40°C. Then, it is necessary to add 1°C correction.
6.4.5. Hold the TIME RUN/STOP key pressed for more than 8 s to activate calibration mode (figure 4).
6.4.6. Using the ▲ and ▼ T, °C keys, change the calibration coefficient (fig. 5/1) so that the new temperature value (fig. 5/2) corresponds to the independent sensor temperature. In our example, the calibration coefficient will be 0.974.
Note. Calibration coefficient can be changed in range from 0.936 to 1.063 (±0.063), with increment of 0.001. This calibrating coefficient will correct temperature through all the operation range.
Note. Coefficient value changes are recommended after the unit has reached 30°C temperature.

6.4.7. Press the ►/■ RUN/STOP key once to save the changes and exit the calibration.
6.5. The display will show calibrated temperature as shown on fig. 6/1 and the unit will continue thermal stabilization according to the previously set temperature.

Figure 5. Changing the coefficient:

1. Calibration coefficient;
2. Temperaturewith current coefficient

Figure 6. Display after calibration:

1. Set temperature;
2. Current calibrated temperature

Specifications

The unit is designed for operation in cold rooms, incubators (excluding CO2 incuba- tors) and closed laboratory rooms at ambient temperature from +4°C to +40°C in a non- condensing atmosphere and maximum relative humidity 80% for temperatures up to 31°C decreasing linearly to 50% relative humidity at 40°C. Operating altitude above sea level is up to 2000 m.
Biosan is committed to a continuous programme of improvement and reserves the right to alter design and specifications of the equipment without additional notice.

to +100°C
Setting resolution| 0.1°C
Stability2, at +37°C| ±0.1°C
Maintaining accuracy2, at +37°C| ±0.5°C
Uniformity over the plat-
form2,| at +4°C| —| ±0.6°C
at +37°C| ±0.1°C| ±0.1°C
at +100°C| ±0.2°C| ±0.3°C
Average heatin, speed from +25°C to +100°C| 4°C/min| 5°C/min
Average cooling from +100°C to +25°C| —| 5°C/min
speed from +25°C to +4°C| —| 1.8°C/min
Calibration option| yes
Calibration coefficient range| 0.936…1.063 (± 0.063)
General parameters| TS-100 I TS-100C
---|---
Speed range| 250-1400 rpm
Speed setting resolution| 10 rpm
Maximal speed
deviation| for 250 rpm| 2%
for 1400 rpm| 0.7%
Orbit| 2 mm
Digital time setting| 1 min – 96 h
Time setting and countdown resolution| 1 min
Maximal continuous operation times| 168 h
Display| 16×2 symbols, LCD
Dimensions| Without thermoblock, LxWxH| 220x240x90 mm
With thermoblock, LxWxH.| 220x240x130 mm
Electrical parameters| TS-100 I TS-100C
---|---
Overvoltage category| II
Pollution degree| 2
Input voltage and current / power consumption| 12 V, 3.5 A / 42 W 1 12 V, 5.0 A / 60 W
External power supply| in AC 100-240 V, 50/60 Hz, out DC 12 V
Weight’| 3.7 kg 1 4.8 kg

1.  Room temperature
2. Data for 75% filled tubes or microplates
3. Recommended interval between prolonged operation sessions not less than 1 hour
4. Accurate within ± 10%.

Ordering information

8.1. Models and versions available:

8.2. To inquire about or order the optional accessories or the replacement parts, contact Biosan or your local Biosan representative.
8.2.1. Optional thermoblocks for TS-100:

8.2.2. Optional thermoblocks for TS-100C:

Caution! Thermoblocks for TS-100 and TS-100C are not interchangeable!
  Installing a thermoblock from different model will irreversibly damage both the unit and the thermoblock! TS-100C thermoblocks have an additional sticker.

8.2.3. Universal replacement parts for TS-100 and TS-100C

Care and maintenance

9.1. Service.
9.1.1. If the unit is disabled (e.g., no platform motion, no heating, no reaction to key presses, etc) or requires maintenance, disconnect the unit from the mains and contact Biosan or your local Biosan representative.
9.1.2. All maintenance and repair operations (except listed below) must be performed only by qualified and specially trained personnel.
9.1.3. Operating integrity check. If the unit follows the procedure described in sections
5. Operation and 6. Calibration, then no additional checks are required.
9.1.4. Cleaning and disinfection. Cleaning and decontamination may be necessary as a safeguard when laboratory heating equipment and any accessories are maintained, repaired, or transferred. We recommend keeping a checklist of completed tasks, with dates and additional information, as the means of confirmation. The instructions state that the RESPONSIBLE BODY must ensure that:

•  appropriate decontamination is carried out if hazardous material is split onto or into the equipment;
• no decontamination or cleaning agents are used which could cause a HAZARD as a result of a reaction with parts of the equipment or with material contained in it;
• the manufacturer or his representative is consulted if there is any doubt about the compatibility of decontamination or cleaning agents with parts of the equipment or with material contained in it.

9.1.5. Use mild soap and water with a soft cloth or sponge for cleaning the exterior. Rinse remaining washing solution with distilled water. Wipe dry the excess water with clean, soft cloth or sponge. 9.1.6. To disinfect the exterior plastic parts, use 75% ethanol or DNA/RNA removing solution (e.g., Biosan PDS-250). After disinfecting, wipe dry the surfaces.
9.1.7. The unit and its accessories are not autoclavable. 9.2. Disposal. Disposal of the appliance requires special precautions and must be carried out at an appropriate disposal site, separate from normal household waste. To prevent pollution of the environment, all waste resulting from the disposal of the product must be collected and disposed of in the country of use, in accordance with the applicable requirements for the handling of electronic waste.
9.3. Rubber belt replacement. For maintenance of reliable operation of the device, the manufacturer recommends replacing rubber belts after 1.5 years or 2000 hours of operation time. – Disconnect the external power supply from the device. – Remove 4 fixation screws on the device bottom and remove the bottom plate. – Replace the rubber belt (fig. 7). – Reassemble the device.

9.4. Power failure. In the event of the power failure, on restoring power, unit restarts thermal stabilisation. Shaking and timer are reset and must be restarted manually.
9.5. Error codes in case of a defect. Some malfunctions trigger an error code to appear on display, accompanied by a sound signal every 8 s. Press the ►/■ RUN/STOP key
to turn off the signal. Error code format is letters ER and a single digit. Disconnect the unit from the electric circuit and report the error code to Biosan or your local Biosan
representative.

Warranty

10.1. The manufacturer guarantees the compliance of unit with the requirements of specifications, if the customer follows the operation, storage and transportation instructions. 10.2. The warranted service life of unit from date of delivery to the customer is 24 months. For extended warranty, register the unit, see 10.5.
10.3. Warranty covers only the units transported in the original package.
10.4. If any manufacturing defects are discovered by the Customer, an unsatisfactory equipment report shall be compiled, certified and sent to the local distributor address. To obtain the claim form, visit section Technical support on our website at link below.
10.5. Extended warranty. For TS-100 and TS-100C, the Premium class models, one year of extended warranty is available free of charge after registration, during 6 months from the date of sale. Online registration form can be found in section Warranty registration on our website at the link below.
10.6. Description of the classes of our products is available in the Product class description section on our website at the link below.

http://biosan.lv/en/support
biosan.lv/en/support

|
---|---

http://biosan.lv/register-en
biosan.lv/register-en

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http://biosan.lv/classes-en 
biosan.lv/classes-en

10.7. The following information will be required in the event that warranty or post-warranty service comes necessary. Complete the table below and retain for your records.

TS-100 / TS-100C Thermo-shaker for microtubes and
microplates| |

Declaration of conformity

11.1. Thermo-Shakers TS-100 & TS-100C are in conformity with the following relevant Un- ion legislations:

LVD 2014/35/EU| LVS EN 61010-1:2011 + A1:2019 Safety requirements for electrical equipment for measurement, control, and laboratory use. General requirements. LVS EN 61010-2-010:2020 Particular requirements for laboratory equipment
for heating of materials. LVS EN 61010-2-051:2021 + A11:2021 Particular requirements for laboratory equipment
for mixing and stirring.
---|---
EMC 2014/30/EU| LVS EN 61326-1:2021 Electrical equipment for measurement, control and laboratory use. EMC requirements.
General requirements.
RoHS3 2015/863/EU| Directive on the restriction of the use of certain hazardous substances in electrical and electronic equipment.
WEEE 2012/19/EU| Directive on waste electrical and electronic equipment.

how to  choose
A PROPER SHAKER ROCKER VORTEX

SIA Biosan
Ratsupites 7 k-2, Riga, LV-1067, Latvia
+371 67426137,
fax: +371 67428101
[email protected]
http://www.biosan.lv

References

• Product classes | Biosan
• Technical support | Biosan
• Warranty registration | Biosan

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