bioSan DEN-600 Laboratory Photometer Instructions

bioSan DEN-600 Laboratory Photometer

About this edition of user instructions

The current edition of the user instructions applies to the following models and versions of photometers:

• DEN-600 ……………………………………………………………………………………. version V.1AW 

Safety precautions

Caution!
Make sure you have fully read and understood the present Manual be-fore using the equipment. Please pay special attention to sections marked by this symbol.

GENERAL SAFETY

•  The protection provided can be ineffective if the operation of the appliance does not comply with the manufacturer’s requirements.
•  Save the unit from shocks and falling.
•  Store and transport the unit at ambient temperatures between -20°C and +60°C and maximum relative humidity of 80%.
•  After transportation or storage and before connecting it to the electric circuit, keep the unit under room temperature for 2-3 hrs.
•  Use only original parts and accessories, provided by the manufacturer for this product.
•  Before using any cleaning or decontamination methods except those recommended by the manufacturer, check with the manufacturer that the proposed method will not damage the equipment.
•  Do not make modifications in the design of the unit.

ELECTRICAL SAFETY

•  Connect only to the mains with voltage corresponding to that on the serial number label.
• Use only the external power supply provided with this product.
•  Ensure that the power plug is easily accessible during use.
•  Disconnect the unit from the mains before moving.
•  If liquid penetrates into the unit, disconnect it from the mains and have it checked by a repair and maintenance technician.
•  Do not operate the unit in premises where condensation can form. The operating conditions of the unit are defined in the Specifications section.

DURING OPERATION

•  Do not operate the unit in environments with aggressive or explosive chemical mixtures. Please contact the manufacturer for possible operation of the unit in specific atmospheres.
•  Do not operate the unit if it is faulty or has been installed incorrectly.
•  Do not use outside laboratory rooms.

BIOLOGICAL SAFETY

• The user is responsible to carry out appropriate decontamination if hazardous material spills on or penetrates into the equipment.

General information

DEN-600 is a compact, portable, rechargeable battery-powered photometer. It comprises of 600 nm wavelength optical system, which enables to application –

1. the OD600 method that estimated total number of cells, 2) McFarland (McF) turbidity measurement method, 3) the Brad-ford protein assay method for protein concentration measurement. The device serves as an inexpensive alternative to a spectrophotometer, which is commonly used for these applications. Because DEN-600 is battery-powered and compact, it can be comfortably located in a biosafety cabinet, anaerobic chamber or quickly moved to another lab room. Additionally, the vessel holding mechanism allows accommodating round bottom, conical vials or falcon tubes, therefore enabling to measure the absorbance (Abs) and turbidity in Abs, OD and McFarland units. USB connectivity and DEN software allow for data transfer, data processing and calculation, software calibration for the Bradford protein assay method or a custom calibration for a specifically applicable vessel.

COMMON APPLICATIONS:

•  Cell concentration measurement
•  Cell growth data estimation
•  Log phase estimation for microbial cells induction
•  Competent cell preparation
•  Bradford protein assay method
•  Antibiotic susceptibility testing
•  Inhibitory tests

Getting started

Unpacking.
Remove packing materials carefully and retain them for future shipment or storage of the unit. Examine the unit carefully for any damage incurred during transit. The warranty does not cover in-transit damage. The warranty covers only the units trans-ported in the original package.

Complete set. Package contents:

Standard set:

• DEN-600 photometer ……………………………………………………………………………….. 1 pce.
• External power supply ……………………………………………………………………………… 1 pce.
• USB cable for connection to PC ………………………………………………………………… 1 pce.
• PC software and instructions, on USB disk drive …………………………………………. 1 pce.
• User instructions, declaration of conformity …………………………………………………1 copy

Optional accessories

• Abs Calibration set …………………………………………………………………………….. on request
• CKG16 McF calibration kit for Ø 16 mm glass tubes …………………………….. on request
• McF calibration kit for Ø 12 mm glass tubes …………………………………………. on request
• Glass sample tubes, set of 78 pcs …………………………………………………… on request

Setup.

•  Make sure that no direct overhead light enters the socket.
•  Connect the external power supply unit into the socket at the rear side of the unit and position the unit for easy access to the external power supply and the power switch.

Note:
Connecting an external power supply is optional when running from the built-in battery.

•  Remove the protective film from the display.

Factory calibration.
The device is pre-calibrated at the factory for operation with the glass tubes 16 mm in external diameter at a temperature range from +15°C to +25°C and saves calibration data when being switched off.

Attention! Recalibrate the unit before using the tubes that are different from factory-calibrated (e.g. with different outer diameter, bottom shape or different material, e.g., plastic, glass thickness). See the Calibration section of this manual.

Operation

Recommendations during operation

•  Remove the tube with the solution from the socket before switching the unit on or off.
•  Keep the unit switched on for 15 minutes before starting the operation in order to stabi-lize it in the working mode.
•  If required, agitate the reaction vessel by pipette or vortexing the reaction vessel by, e.g. Biosan pipettes or Biosan V-1 plus respectively.

Connect the external power supply to electric circuit. Switch on the unit using the green Power key (fig. 1/4) on the control panel.

Note:
Connecting external power supply is optional when running from the built-in battery or when connected to PC.

The following indication may be shown on the display (fig. 1/1):

•  Battery indicator, top left corner. Animated when charging.
• USB data transfer, bottom right corner. Appears only when connected to PC and during data transfer.
•  Two measurement modes for operation are available – McFarland (McF) and Abs (Absorbance) see Figures 2 and 5.3.
•  Request for baseline calibration, see 5.3.
•  Current operation mode, see 5.4.

Measurement mode selection  Select measurement units by pressing Reset key for McF (fig. 1/5) and Mode key for Abs (fig. 1/3).

Baseline calibration. The unit requires an initial baseline (only in Abs mode), e.g. depending on the reaction vessel or suspension colour. Insert the reaction vessel with or without suspension into the unit and press the Baseline key (fig. 1/2).

Choosing the mode of operation. Press the Mode key (fig. 1/3) to circle through available operation modes, Sample, Save and Read.

Sample mode – measurement without saving the results. Place the sample in the unit and press the Measure key (fig. 1/6). Display shows the measurement result.

Save mode – measurement and saving of measurement results. An indication S# ap-pears in the top right corner, where # is a number from 0 to 999. Place the sample in the unit and press the Measure key. Display shows the measurement result and saves it to internal memory that can be uploaded to dedicated PC software (see the software manual).

Read mode – view the saved measurements. An indication R0 appears in the top right corner, and the corresponding value – in the middle of the display. Press the Measure key to see the next saved measurement value. Press the Reset key to move back to the previously saved value (R0).

Creating McF user calibration
The device is pre-calibrated at the factory for operation with the glass tubes 16 mm (or other if separately requested) in external diameter at temperature range from +15°C to +25°C and saves calibration data when being switched off.

The standard suspensions must be sufficiently resuspended before measurement. To do this, proceed as follows:

• Mix adequately at the highest RPM setting of your vortex mixer device, i.e. Biosan V- 1 plus.
•  Invert the tubes carefully before comparison. Avoid bubble formation during operation.
• Additionally, McF standards preparation/mixing before measurement can vary from different manufacturers, we advise following those requirements thoroughly to achieve the best results.

Perform calibration from lower to higher calibration value. Use at least 2 points for calibration. Different calibration points are available – 0.00, 0.50, 1.00, 2.00, 3.00,
4.00, 5.00, 6.00, 7.00, 8.00, 9.00, 10.00, 11.00, 12.00, 13.00, 14.00, 15.00, 16.00.

•  The minimum requirement is 5 points closest to the working range limits (e.g. 0.00 and 6.00 for operation in 0.00 – 6.00 McF range). The best results are obtained when maximum amount of calibration points for the required range are used.
• If the standard for 0.00 value is not available, fill the tube (of the kind that is used for operations) with distilled water. Use the tube as the 0.00 value standard.
•  Press Baseline key 5 times in McF mode to enter user calibration regime (Figure 3). 5.6.6. Insert the required McF standard (fig. 3/1) into the socket and press Meas (Measure key). If the required standard is not available press Next (Baseline key) to skip to the next standard.
•  To finish the calibration process press Next (Baseline key) skipping all the not-required calibration points, when asked to save user calibration or not, press Measure key for Yes and Baseline key for No.

Saved user calibration data observation Press Mode key in user calibration regime, then press Next (Baseline key) to observe calibrated points and Abs result equivalent to McF in user calibration.

Restoring to McF factory calibration settings Enter user calibration regime by pressing the Baseline key 5 times in McF measurement mode, press Mode key 2 times, then select between User and Factory calibration settings by pressing Sel (Baseline key, fig. 5/2), to exit the menu press the Reset key.

Resetting the memory. In the Save mode, press the Reset key twice to delete all saved values from the memory.

To control the unit from a PC, please see the software manual. After finishing the operation, switch off the unit using the green Power key. If the external power supply is used, disconnect it from the electric circuit.

Specifications

The unit is designed for operation in cold rooms, incubators (except CO2 incubators) and closed laboratory rooms at ambient temperature from +4°C to +40°C in a non-condensing atmosphere and maximum relative humidity 80% for temperatures up to 31°C decreasing lin-early to 50% relative humidity at 40°C.

For optimum performance, photometer DEN-600 should be sited in a clean, dry, dust-free atmosphere. When in use, ambient temperature, relative humidity and light levels should remain as constant as possible. In order to perform a measurement under the most stable condition and to conform to measurement specifications, the instrument must be used in an air-conditioned room at 20–25°C and non-condensing atmosphere of 30–70% humidity. The manufacturer is committed to a continuous programme of improvement and reserves the right to alter design and specifications of the equipment without additional notice.

Measurement specifications

General specifications

Ordering information

Models and versions available:

To inquire about or order the optional accessories, contact Biosan or your local Biosan representative.

Optional accessories

Calibration set for Abs. Certified reference material, neutral density glass filter set of 4 Abs calibration points – 0.3532, 1,0512, 2,0425, 2,927

(the values may vary slightly from batch to batch)

|

BS-050109-AK

CKG16 , calibration kit for glass tubes 16 mm in diameter.

Latex particles. Set of 0.5, 1.0, 2.0, 3.0, 4.0 McF turbidity standards

| BS-050102-BK
Calibration kit for glass tubes 12 mm in diameter. Polymer particles.

Set of 0.0, 0.5, 2.0, 3.0 McF turbidity standards

| On request
Glass sample tubes, set of 78 pcs.

Outer diameter 16 mm, height 100 mm, wall thickness 0.8 mm

| BS-050102-LK

 Care and maintenance

• If the unit requires maintenance, disconnect the unit from the mains and contact Biosan or your local Biosan representative.
• All maintenance and repair operations must be performed only by qualified and specially trained personnel.
• Standard ethanol (75%) or other cleaning agents recommended for cleaning of laboratory equipment can be used for cleaning and decontamination of the unit.

Warranty

• The Manufacturer guarantees the compliance of the unit with the requirements of the Specifications, provided the Customer follows the operation, storage and transportation instructions.
• The warranted service life of the unit from the date of its delivery to the Customer is 24 months. For an extended warranty, see 9.5.
• The warranty covers only the units transported in the original package.
• If any manufacturing defects are discovered by the Customer, an unsatisfactory equipment report shall be compiled, certified and sent to the local distributor address. To obtain the claim form, visit the Technical support page on our website at link below. Extended warranty. For DEN-600, the Smart class model, an extended warranty is a paid service. Contact your local Biosan representative or our service department through the Technical support section on our website at the link below.
• Description of the classes of our products is available in the Product class description section on our website at the link below.

Technical support

biosan.lv/en/support

Product class description

biosan.lv/classes-en

The following information will be required in the event that warranty or post- warranty service comes necessary. Complete the table below and retain for your records.

DEN-600 ,

photometer

| DEN- 600 ,

photometer

| DEN- 600 ,

photometer

EU Declaration of conformity

• Unit type: Densitometers
• Models : DEN-1, DEN-1B, DEN-600
• Serial number : 14 digits styled XXXXXXYYMMZZZZ, where XXXXXX is model code, YY and MM – year and month of production, ZZZZ – unit number.
• Manufacturer : SIA BIOSAN Latvia, LV-1067, Riga, Ratsupites str. 7/2

The objects of the declaration described above is in conformity with the following relevant Union harmonization legislations:

LVD 2014/35/EU

| LVS EN 61010-1:2011 Safety requirements for electrical equipment for measurement, control, and laboratory use. General requirements.
---|---

EMC 2014/30/EU

| LVS EN 61326-1:2013 Electrical equipment for measurement, control and laboratory use. EMC requirements.

General requirements.

RoHS3 2015/863/EU| Directive on the restriction of the use of certain hazardous substances in electrical and electronic equipment.
WEEE 2012/19/EU| Directive on waste electrical and electronic equipment.

I declare that the Declaration of Conformity is issued under sole responsibility of the manufacturer and belongs to the above-mentioned objects of the declaration.

Biosan SIA
Ratsupites 7, build.2, Riga, LV-1067, Latvia Phone: +371 67426137 Fax: +371 67428101 http://www.biosan.lv
Edition 1.03 – June of 2020

References

• Product classes | Biosan
• Technical support | Biosan
• Home | Biosan

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