biosan CVP-2 Centrifuge Vortex for PCR Plates Owner’s Manual

biosan CVP-2 Centrifuge Vortex for PCR Plates

Product Information

The CVP-2 is a fully automatic device that combines centrifuge and vortex technologies for PCR plates. It uses a Spin-mix-spin (SMS) algorithm to spin down micro volumes of reagents on the well’s bottom, following mixing and spin-down again. The CVP-2 is designed to work with PCR plates with skirts, semi-skirts, or without them. It is a must-have instrument for PCR and DNA analyses laboratory.

Model and Name: CVP-2, centrifuge-vortex for PCR plates
Version: V.3A01

Safety Precautions

It is important to read and understand the user manual before using the equipment. The equipment should not be used without a lid, and the safety cover and lid should be secured as described in section 5.6. The equipment uses direct current. The emergency opening location is described in section 5.18.

Product Usage Instructions

Getting Started

1. Place the unit on an even, stable, horizontal, and clean surface
2. Remove the protective film from the display
3. Plug the power cable into the external power supply
4. Plug the external supply into the power socket on the rear of the unit and position the unit so that there is easy access to the power switch and mains outlet
5. According to EN 61010-2-20, people and hazardous materials must not be within a 300 mm area around the device during the centrifuge operation.

Operation

1. Connect the external power supply to a properly grounded power socket.
2. Turn on the power switch (I position) on the rear side of the unit. The lid unlocks automatically.
3. Press the Open key (fig. 2/1) and open the lid by lifting it upwards by hand (indication will appear in the Mode bottom line, fig. 2/2). The lid will only open when the rotor is stopped.

Optional Accessories

• AP-384 adapter 384-well PCR plates (Eppendorf) – 2 pcs.
• Wrench AP-96
• Wrench AP-384

If you have any feedback on the product or service, please send it to the manufacturer at SIA Biosan Ratsupites 7 k-2, Riga, LV-1067, Latvia. You can also contact them by phone at +371 674 261 37 or fax at +371 674 281 01. For marketing inquiries, email [email protected] and for service inquiries, email [email protected].

Note: CE marking and WEEE directive marking are affixed to the unit and packaging to indicate conformity with European health, safety, and environmental protection standards. See section 12.1 for more information.

About this edition of user instructions

The current edition of the user instructions applies to the following models:

Edition 3.02 – April of 2022

Safety Precautions

Caution! Make sure you have fully read and understood the present Manual before using the equipment. Please pay special attention to the sections marked by this symbol.

Icons used on the unit and packaging

General safety

• This unit is a state-of-the-art piece of equipment, which is extremely safe to operate. Nevertheless, it may lead to danger for users or others nearby if used by untrained staff, in an inappropriate way or for purposes other than for those it was designed.
• Save the unit from shocks or falling.
• Store and transport the unit as described in section 10. Storage and transportation on page 14.
• Use only original accessories provided by the manufacturer and ordered specifically for this model.
• Before using any cleaning or decontamination methods except those recommended by the manufacturer, check with the manufacturer that the proposed method will not damage the equipment.
• Do not make modifications to the design of the unit.

Electrical safety

• Connect only to the external power supply with voltage corresponding to that on the serial number label.
• Use only the external power supply provided with this product.
• Ensure that the external power supply is easily accessible during use.
• Do not plug the unit into an ungrounded mains outlet, and do not use an ungrounded extension lead.
• Disconnect the unit from the electric circuit before moving.
• Disconnect the external power supply from mains outlet to turn off the unit.
• If liquid penetrates into the unit, disconnect it from the external power supply and have it checked by a repair and maintenance technician.
• Do not operate the unit in premises where condensation can form. Operating conditions of the unit are defined in section 7. Specifications on page 11.

During operation

• Do not use rotors with visible signs of corrosion, wear or mechanical damage.
• Do not use the unit without the rotor safety cover.
• Do not operate the unit in environments with aggressive or explosive chemical mixtures. Please contact manufacturer for possible operation of the unit in specific atmospheres.
• Observe the safety area of 300 mm around the unit. Persons and hazardous materials must not be located in the safety area whilst the unit is operating.
• Do not centrifuge flammable or chemically active substances.
• Do not use outside laboratory rooms.
• Do not operate the unit if it is faulty or was installed incorrectly.
• Always fix rotor securely. Stop the operation immediately with the Stop key if any unusual noise occurs during acceleration, which can be due to improper rotor fixation.

Biological safety

• According to EN 61010-2-020, a centrifuge without biological safety system is not considered a biologically safe and therefore cannot be used for centrifuging hazardous materials contaminated with toxic, radioactive or pathogenic microorganisms.
• Take additional measures if a contact of bare skin with hazardous substances is possible. Take care not to cut, puncture, or tear the gloves. Not one glove material is impervious to all substances. Disposable surgical or PVC gloves provide substantial but not complete protection. PVC gloves are probably more protective than surgical gloves, but they are stiffer and less tactile. Discard gloves after each use.
• It is the user’s responsibility to carry out appropriate decontamination if hazardous material spills on or penetrates into the equipment.
• Some inner components are susceptible to contamination. Only qualified specialists, who are experienced in deactivations procedures, must perform cleaning these components for their future use.
• The user is responsible for decontaminate the unit before its decommissioning and utilization.

General Information

After many years of Combined Centrifuge/Vortex concept success, we are proud to introduce the long-awaited Centrifuge/Vortex for PCR plates, CVP-2, to the sample preparation market. The application range of CVP-2 is PCR plates: with skirts, semi-skirts or without them. The Spin-mix-spin technology is intended to spin-down micro volumes of reagents on the well’s bottom (first centrifugation spin), following mixing (mix) and spin-down the reagents again (second spin). We named this repetitive algorithm SMS, spin-mix-spin. It aims at reducing the mistakes during sample preparation for PCR analysis. The algorithm was published for the first time and patented by Biol. Dr. V. Bankovskis (V. Bankovskis et al., Riga, Latvia, Pat. No. P94-74). CVP-2 is a fully automatic device for reproducing SMS for 2 PCR plates at the same time, thus saving time considerably. A must-have instrument for PCR and DNA analyses laboratory.

CVP-2 is 4 devices combined in 1:

1. Centrifuge –Maximum RCF: 175 × g (1500 rpm)
2. Vortex (regulation timer 0 – 60 s)
3. Centrifuge/Vortex
4. SMS for realization of the SMS algorithm

Getting started

Unpacking. Remove packing materials carefully and retain them for future shipment or storage of the unit. Examine the unit carefully for any damage incurred during transit. The warranty does not cover in-transit damage. Warranty covers only the units transported in the original package.

Standard set. Package contents:
Standard set

• CVP-2 Centrifuge/Vortex ……………………………………………………………………….. 1 pce
• R-2MP rotor for PCR plates with fixation nut for fastening and safety cover ….. 1 pce
• AP-96 adapter for semi-skirted and non-skirted PCR plates, 96 wells ………….. 2 pcs
• Wrench for rotor removing …………………………………………………………………….. 1 pce
• The key for cover unblocking (fixed into rear panel of the unit)………………………….. 1 pce
• External power supply …………………………………………………………………………… 1 pce
• Power cable ………………………………………………………………………………………… 1 pce
• User instructions, declaration of conformity …………………………………………….. 1 copy

Optional accessories, on request

AP-384 adapter 384-well PCR plates (Eppendorf) ……………………………………. 2 pcs.

Setup

• Place the unit on the even, stable, horizontal and clean surface.
• Remove protective film from the display.
• Plug the power cable into the external power supply.
• Plug the external supply into the power socket on the rear of the unit and position the unit so that there is easy access to the power switch and mains outlet.
• According to EN 61010-2-20 people and hazardous materials must not be within a 300 mm area around the device during the centrifuge operation.

Rotor installation

• Connect the power cord to a properly grounded mains outlet. Switch on the power switch (I position) on the rear.
• Press the Open key and open the lid by lifting it upwards by hand.
• Unscrew the fixation nut (fig. 1/1) counter clockwise using the wrench included in standard set and remove the nut.
• Place the rotor and secure it tightly with the fixation nut, placing the nut with the key holes up (fig.1/1) and turning it clockwise by the included wrench.
• Place the safety cover (fig. 1/2) on the rotor by pressing the cover clip (fig. 1/3) down.
• Close the centrifuge lid.
  • NOTE: To close the lid, press down the middle part of the edge of the lid until the clicking sound is heard. Otherwise, the lid will not be secured properly, which will cause operation malfunction.
• Turn off the centrifuge with switch on the rear (position O).

Operation

• Recommendations during operation.
  • Do not use rotors with visible signs of corrosion, wear or mechanical damage. Check the rotor, buckets for any signs of wear or corrosion, and replace if necessary.
  • When using semi-skirted or non-skirted PCR plates, put the plate into an appropriate adapter. Insert the plate into the rotor with the adapter. Refer to section 6. How to choose a proper adapter.
  • Load both PCR plates to balance the unit during operation.
  • Before operation check PCR plates and ensure that they are closed properly. Content can be spilled from open PCR plates, endangering personnel working with hazardous materials.
  • For proper mixing, it is recommended to fill PCR plates not more than 75% of their nominal volume.
  • Seal the microplates with an appropriate adhesive film to avoid spilling the sample.
• Connect the external power supply to a properly grounded power socket. Turn on the power switch (I position) on the rear side of the unit. The lid unlocks automatically.
• Press the Open key (fig. 2/1) and open the lid by lifting it upwards by hand (indication will appear in the Mode bottom line, fig. 2/2). The lid will open only with the rotor stopped.
• Remove the safety cover by lifting the safety cover clip.
• Insert TWO micro-test plates in rotor one opposite another.
• Place the safety cover on the rotor and secure it by pressing the safety cover clip down tightly. Close the outer lid carefully and smoothly until the clicking sound is heard (an indication will appear in the Mode bottom line).
  • Note: To close the lid, press down the middle part of the edge of the lid until the clicking sound is heard. Otherwise, the lid will not be secured properly, which will cause an operation malfunction.
• Quick centrifugation. Press the QS (fig. 2/10) button for quick mixing/sedimentation and hold it pressed for the desired time. The unit will automatically when the QS button is released.

Setting parameters

• By pressing the Select key (fig. 2/3) choose a parameter to change. Each pressing of the Select key consecutively activates parameters in the operation cycle; currently active parameter is indicated by blinking.
• Use ▼ and ▲ keys (fig. 2/4) for setting the corresponding values. Pressing a key for longer than 2 seconds will increase the change rate.
• Speed (RPM) and spinning/mixing time (Time) can be changed during operation — when a new operation cycle starts, the microprocessor automatically accepts changes as the current operation mode. Cycle count (Cycle) and operating mode (Mode) cannot be changed during operation.
• Mode (fig. 3/1) parameter can be one of the following:
  • SMS SMS-algorithm mode.
  • MS alternating centrifugation and vortexing.
  • SPIN centrifugation only.
  • MIX vortexing only.

SMS-algorithm

• Select the SMS-algorithm (indication S.M.S. of Mode parameter, fig. 3/6).
• Set the required centrifugation speed, from 300 to 1500 rpm (increment 100 rpm, fig. 3/2).
• Set the required centrifugation time, from 1 s to 30 min (increment 1 s, after 1 min – 1 min, fig. 3/1).
• Set the required vortexing speed, from 300 to 1200 rpm (increment 100 rpm, fig. 3/4).
• Set the required vortexing time, from 1 to 60 s (increment 1 s, fig. 3/3).
• Set the required SMS-algorithm cycle count, from 1 to 999 (fig. 3/5).

Centrifugation + vortexing

• Select the alternating centrifugation and vortexing algorithm (indication M.S. of Mode parameter, fig. 4/6).
• Set the required centrifugation speed, from 300 to 1500 rpm (increment 100 rpm, fig. 4/2)
• Set the required centrifugation time, from 1 s to 30 min (increment 1 s, after 1 min – 1 min, fig. 4/1).
• Set the required vortexing speed, from 300 to 1200 rpm (increment 100 rpm, fig. 4/4).
• Set the required vortexing time, from 1 to 60 s (increment 1 s, fig. 4/3).
• Set the required SMS-algorithm cycle count, from 1 to 999 (fig. 4/5).

Centrifugation

• Select the centrifugation mode (indication SPIN of Mode parameter, fig. 5/3).
• Set the required centrifugation speed, from 300 to 1500 rpm (increment 100 rpm, fig. 5/2)
• Set the required centrifugation time, from 1 s to 30 min (increment 1 s, after 1 min – 1 min, fig. 5/1).

Vortexing

• Select the vortexing mode (indication MIX of Mode parameter, fig. 6/3).
• Set the required vortexing speed, from 300 to 1200 rpm (increment 100 rpm, fig. 6/2).
• Set the required vortexing time, from 1 to 60 s (increment 1 s, fig. 6/1).
• Press Run key (fig. 2/5) to start operation.
• Rotor will begin rotating and, on the display, indication (fig. 2/2), cycle countdown (fig. 2/6) and changing parameters of the current mode (fig. 2/7 and fig. 2/8).
• Operation can be stopped before the set number of cycles elapses, if necessary, by pressing the Stop key (fig. 2/9).
• Unit stops automatically after executing set number of cycles (blinking indication on display, fig. 2/2) and the lid unlocks automatically. A sound signal is emitted after full stop of the rotor. Press Stop key (fig. 2/9) to stop the signal.
• At the end of operation set the power switch in position O (OFF) on the rear. Disconnect the external power supply from electric circuit.
  • Note: The electrical lid lock allows opening the lid only when the unit is connected to the mains and is turned on.
• Lid emergency opening. Disconnect the power cord from external power supply unit. Wait until the rotor stops completely. Insert the provided lid-unblocking key (found on the rear panel of the unit) into the marked opening on the right side of the unit and press to unlock the lid.

How to choose a proper adapter

Displayed in the table below are PCR plate types in relation to available adapters.

Caution! PCR plate height inside an adapter should not be bigger than 17 mm.

Specifications

The unit is designed for operation in cold rooms, incubators and closed laboratory rooms at ambient temperature from +4°C to +40°C in a non-condensing atmosphere and maximum relative humidity 80% for temperatures up to 31°C decreasing linearly to 50% relative humidity at 40°C. Biosan is committed to a continuous program of improvement and reserves the right to alter design and specifications of the equipment without additional notice.

Speed setting range

• Centrifugation ………………………………………………………………………….. 300–1500 rpm
• Vortexing ………………………………………………………………………………… 300–1200 rpm
• Speed setting increment …………………………………………………………………….100 rpm
• Relative centrifugal force …………………………………………………………….. up to 175 x g
• Centrifugation mode time range ……………………………………………………. 1 s – 30 min
• Centrifugation mode time increment ………………………………. 1 s; after 1 min – 1 min
• Vortex mode time range ………………………………………………………………………. 0–60 s
• Vortex mode time increment ……………………………………………………………………… 1 s
• Number of programmable cycles …………………………………………………. 1–999 cycles
• Maximum plate height …………………………………………………………………………. 17 mm
• Display ………………………………………………………………………… LCD, 2×16 characters
• Safety measures …………………………………………………….Safety cover, lid with a lock
• Dimensions ……………………………………………………………………….. 285x350x190 mm
• Operating voltage and current ………………………………………………………. 12 V=, 1.5 A
• Power consumption ………………………………………………………………………………. 18 W
• External power supply input …………………………. in 100–240V~, 50/60 Hz, out 12 V=
• Weight, accurate within ± 10% …………………………………………………………….. 6.15 kg

Ordering information

Models and versions available.

To inquire about or order the optional accessories or the replacement parts, contact Biosan or your local Biosan representative.
Optional accessory

Replacement parts

BS-010219-DK

Care and maintenance

• Service
  • If the unit is disabled (e.g., no centrifugation or vortexing, no reaction to key presses, etc) or requires maintenance, disconnect the unit from the mains and contact Biosan or your local Biosan representative.
  • All maintenance and repair operations (except listed below) must be performed only by qualified and specially trained personnel.
  • Operating integrity check. If the unit follows the procedure described in section 5. Operation, then no additional checks are required.
• Cleaning and disinfection.
  • Use mild soap and water with a soft cloth or sponge for cleaning the exterior. Rinse remaining washing solution with distilled water. Wipe dry the excess water with clean, soft cloth or sponge.
  • To disinfect the plastic parts, use 75% ethanol or DNA/RNA removing solution (e.g., Biosan PDS-250). After disinfecting it is necessary to wipe the surfaces dry.
  • To access the rotor, open the lid. For powered unit, see 5.3. For unpowered unit, see 5.18. Hold the rotor with one hand, unscrew rotor fixation nut, turning it counter clockwise using included wrench. Retrieve the rotor, clean, and disinfect, as necessary. Replace the rotor and secure it tightly with the fixation nut, placing the nut with the key holes up and turning it clockwise by securing wrench. Replace the rotor safety cover on the rotor by pressing the cover clip down tightly.
  • The rotor and adapters are autoclavable (15 min 121°C), the unit itself is not autoclavable.
  • Disposal. Disposal of the appliance requires special precautions and must be carried out at an appropriate disposal site, separate from normal household waste. To prevent pollution of the environment, all waste resulting from the disposal of the product must be collected and disposed of in the country of use, in accordance with the applicable requirements for the handling of electronic waste.

Storage and transportation

• Store and transport the unit in a horizontal position (see package label) at ambient temperatures between -20°C and +60°C and maximum relative humidity of 80%.
• After transportation or storage and before connecting it to the electric circuit, keep the unit under room temperature for 2-3 hrs.
• For extended storage, the unit does not require special procedures.

Warranty. Production date

• The Manufacturer guarantees the compliance of unit with the requirements of Specifications, provided the Customer follows the operation, storage and transportation instructions.
• The warranted service life of unit from date of delivery to the Customer is 24 months (excluding optional parts mentioned in the table in the Specifications section). For extended warranty, register the unit, see 11.5.
• Warranty covers only the units transported in the original package.
• If any manufacturing defects are discovered by the Customer, an unsatisfactory equipment claim shall be compiled, certified and sent to the local distributor address. To obtain the claim form, visit section Technical support on our website at link below.
• Extended warranty. For CVP-2, a Premium class model, one year of extended warranty is available free of charge after registration, during 6 months from the date of sale. Online registration form can be found in section Warranty registration on our website at the link below.
• Description of the classes of our products is available in the Product class de-scription section on our website at the link below.
• The following information will be required in the event that warranty or post-warranty service comes necessary. Complete the table below and retain for your records.

• Production date. Production date is placed in the serial number, on the label of the unit. Serial number consists of 14 digits styled XXXXXXYYMMZZZZ, where XXXXXX is model code, YY and MM – year and month of production, ZZZZ – unit number

EU Declaration of conformity

• Centrifuge-vortex for PCR plates CVP-2 is in conformity with the following relevant Union legislations:

LVD 2014/35/EU

| LVS EN 61010-1:2011 Safety requirements for electrical equipment for measurement, control, and laboratory use.

General requirements.

LVS EN 61010-2-020:2016 Particular requirements for laboratory centrifuges.

---|---

EMC 2014/30/EU

| LVS EN 61326-1:2013 Electrical equipment for measurement, control and laboratory use. EMC requirements.

General requirements.

RoHS3 2015/863/EU

| Directive on the restriction of the use of certain hazardous substances in electrical and electronic equipment.
WEEE 2012/19/EU| Directive on waste electrical and electronic equipment.

• Declaration of Conformity is available for download on the page for the relevant model on our website by links below, in the Downloads section:
• SIA Biosan
• Ratsupites 7 k-2, Riga, LV-1067, Latvia
• Phone: +371 67426137 Fax: +371 67428101
• https://biosan.lv.
• Edition 3.02 — April of 2022
• Find out about our special offer on
• https://biosan.lv/classes-en.

If you have any feedback on our products or services, we would like to hear from you. Please send all feedback to:

Manufacturer:

• SIA Biosan
• Ratsupites 7 k-2, Riga, LV-1067, Latvia
• Phone : +371 674 261 37
• Fax: +371 674 281 01
• https://biosan.lv.
• Marketing: [email protected].
• Service: [email protected].

References

• Product classes | Biosan
• Technical support | Biosan
• Warranty registration | Biosan
• Home | Biosan
• Home | Biosan
• Product classes | Biosan
• CVP-2, Centrifuge/Vortex for PCR plates| Biosan

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