biosan ES-20 Orbital Shaker Incubator Instruction Manual

biosan ES-20 Orbital Shaker Incubator Instruction Manual

About this edition of user instructions

The current edition of the user instructions applies to the following models and versions of the orbital shaker-incubator:

• ES-20/80: V.1AD

Safety precautions

The following symbols mean:

Caution!
Make sure you have fully read and understood the present Manual before using the equipment. Please pay special attention to sections marked by this symbol.

Caution!
Hot surface! Incubator surface and inner camera may become very hot during use. Always use protective cotton gloves to install or remove samples when the temperature is set higher than 60°C.

GENERAL SAFETY

• The protection provided can be ineffective if the operation of the appliance does not comply with the manufacturer’s requirements.
• Save the unit from shocks and falling.
• After transportation, storage or in the case of condensation formation, and before connecting it to the electric circuit, keep the unit under room temperature for 2-3 hrs to dry out the unit. Without drying out, the operation of the unit may not comply with all safety requirements.
• Store and transport the unit at ambient temperatures between -20°C and +60°C and maximum relative humidity of 80%.
• Before using any cleaning or decontamination methods except those recommended by the manufacturer, check with the manufacturer that the proposed method will not damage the equipment.
• Do not make modifications in design of the unit.

ELECTRICAL SAFETY

• Connect only to the mains with voltage corresponding to that on the serial number label.
• Do not plug the unit into an ungrounded power socket, and do not use an ungrounded extension lead.
• Ensure that the power plug is easily accessible during use.
• Disconnect the unit from the mains before moving.
• If liquid penetrates into the unit, disconnect it from the mains and have it checked by a repair and maintenance technician.
• Do not operate the unit in premises where condensation can form. Operating conditions of the unit are defined in the Specifications section.

DURING OPERATION

• Make sure that all sample vessels are tightly sealed. Humidity caused by evaporation from unsealed vessels inside the incubator will damage the unit.
• Do not operate the unit in environments with aggressive or explosive chemical mixtures. Please contact manufacturer for possible operation of the unit in specific atmospheres.
• Do not incubate flammable, explosive or chemically active substances. If such substance is spilled inside the incubator, it must be cleaned with a moist cloth and a mild soap solution.
• Do not operate the unit if it is faulty or has been installed incorrectly.
• Do not use outside laboratory rooms.
• Do not place a load exceeding the maximum load value mentioned in the Specifications section of this manual.

BIOLOGICAL SAFETY

• The user is responsible to carry out appropriate decontamination if hazardous material spills on or penetrates into the equipment.

General information

ES-20/80 shaker-incubator for biotechnological and pharmaceutical laboratories is a professional category equipment. The typical applications include – microbial and cell culture cultivation, protein expression, solubility studies, general mixing, as well as other various applications in the fields of biology and chemistry. The unit is equipped with a newly developed triple eccentric mechanism for platform motion that provides supreme balancing characteristics, superior reliability and quiet operation. The achieved stability of the unit during vigorous mixing allows for stacking installation of up to 3 units which enables to save space.

The new display and easy to use user interface provide a clear and intuitive control of parameters and allow data logging, storage and display over time. Additional features like out of balance sensor and automatic thermostat failure detection make this shaker-incubator an advanced and safe product. Bluetooth connectivity to PC allows for data management, data logging, parameter control and profiling in a dedicated software that can be requested separately.

A built-in heat-resistant brushless fan provides precise temperature distribution inside the chamber (from 5°C above ambient up to +80°C). Additionally, excellent sample temperature uniformity of ±0.5 °C at 37 °C is achieved. The inner chamber is made of stainless steel. State-of-the-art motor, thermal insulation materials and parameter PID control decrease the energy consumption and make the shaker-incubator highly energy efficient despite its relatively large size.

Getting started

Unpacking

Remove packing materials carefully and retain them for future shipment or storage of the unit. Examine the unit carefully for any damage incurred during transit. The warranty does not cover in-transit damage. Warranty covers only the units transported in the original package.

Caution! Due to the high weight of the unit, its unpacking and installing must be carried out by two persons.

Caution!   After transportation, storage or in the case of condensation formation, and before connecting it to the electric circuit, keep the unit under room temperature for 2-3 hrs to dry out the unit. Without drying out, the operation of the unit may not comply with all safety requirements.

Complete set

Package contents:

Standard set:

• ES-20/80, Orbital shaker-incubator 1 pce.
• Power cable 1 pce.
• Spare fuse (inside fuse holder) 1 pce.
• Four screws and a hex driver 1 set
• USB drive with software and software manual 1 pce.
• Operating instructions, declaration of conformity 1 copy

Optional accessories:

• HSP-30/100 platform on request
• HSP-16/250 platform on request
• HSP-9/500 platform on request
• HSP-6/1000 platform on request
• PP-400 platform on request
• UP-168 universal platform on request
• HSC-50, HSC-100, HSC-250, HSC-500, HSC-1000 clamps for UP-168 ..on request
• TR-21/50 test tube rack for UP-168 on request
• TR-44/15 test tube rack for UP-168 on request
• USB Bluetooth adapter for PC 1 pce.
• Stacking kit for two units on request
• Stacking kit for three units on request
  HSP-30/100

HSP-16/250

HSP-9/500

HSP-6/1000|

PP-400

UP-168

TR-21/50

TR-44/15

Setup

Note.
Ensure that the unit is placed on level horizontal surface, which is able to support its weight.

• Place the unit on an even horizontal stable non-flammable surface 30 cm away from any flammable materials, clear 20 cm around the unit on all sides for ventilation.
• Level the unit on the surface by adjusting the threaded legs. Rotate the legs until the unit is stable, then secure the thread with a jam nut. The unit should stand on all 4 legs, vibrations should be absent.
• Remove the protective film from the display.
• Connect the power cable to the socket on the rear side of the unit and position it with easy access to the power switch and plug.

Platform installation.

1. Remove the silicon mat and unscrew four screws from the previous platform using a hex driver, if a platform was present. Install and secure the new platform to the moving part of the base with four screws and replace the silicone mat.
2. UP-168 platform. The platform can be outfitted with flask clamps or a test tube rack. Arrange the components symmetrically in relation to the center of the platform. Fix them in place with screws included with components. Maximum number of clamps or racks can be found in the table below.
   FC-50| 36
   ---|---
   FC-100, FC-250| 16
   FC-500| 8
   FC-1000| 4
   TR-21/50, TR-44/15| 2

Stacking

Note.
The following section applies to the optional stacking kit, to use with 2 or 3 shaker-incubator units.

Stacking kit contains:

Aquiring a kit. To purchase the kit, contact Biosan or your local Biosan representative, with information from the section Ordering information.

Getting started.

1. Unpack the kit. Unpower the units and disconnect the power cables.

2. Prepare the working space. Ensure that the floor and the desk supports at least 150 kgF/m2 (for 2 units) or 200 kgF/m2 (for 3 units). Laminate or rubber flooring may reduce overall stability and are generally not recommended.

3. Position the units so that the access to the mains plugs, the sockets and the power switches is unobstructed.

4. Stacking two units (figure 2).

5. Set one unit atop the other.

6. Connect the units with four plates. A plate requires four screws, each with a washer and a lock washer on it (fig. 3). Fix the screws tightly.

7. Stacking three units.

8. Base unit (figure 4):
   Attach the left and the right legs to the base unit. A leg requires four screws, each with a washer and a lock washer on it (fig. 3). Fix the screws tightly.
   Regulate the rubber parts of the legs so that the base unit is stable and level.
   Middle unit (figure 5):

9. Set the middle unit atop of the base unit.
   Connect the units with four plates. A plate requires four screws, each with a washer and a lock washer on it (fig. 3). Fix the screws tightly.

10. Top unit (figure 6):

11. Set the top unit atop of the middle unit.

12. Connect the units with four plates. A plate requires four screws, each with a washer and a lock washer on it (fig. 3). Fix the screws tightly.

13. Connect the power cables to the units and to grounded mains sockets.

14. Maintenance. Check the screw fixation and unit stack stability once a month.
    **Figure 2. Stacking two units

Figure 3. Washers on the screw

Figure 4. Stacking three units, base level

Figure 5. Stacking three units, middle level

Figure 6. Stacking three units, top level
**

Operation

1. Connect the unit to a grounded power socket. Set the Power switch on the rear panel to position I (on).

2. The display turns on. From top to bottom, display shows:

   • Current date and time;

   • Actual and set temperatures;

   • Actual RPM, set RPM and timer;

   • Status bar;

   • Context buttons, corresponding to unmarked keys below the display.
     Below the display:

   • Four unmarked keys, corresponding to context buttons on the display;

   • The Select, ▲, ▼ and Run Stop keys.

3. Setting the parameters. Press the Select key to select the parameter to change. Use the ▲ and ▼ keys to adjust the parameter.
   Pressing the key for longer than 2 s increases changing speed. Press the Select key again to select the next parameter. Changes are saved automatically.

4. Setting temperature. Temperature starts changing according to set value. Increment is 0.1ºC. Values can be changed anytime.

5. Setting speed (RPM). Increment is 10 RPM. Speed can be adjusted anytime.

6. Setting time. Time is set separately for hours and minutes, with increment of 1 minute.

7. Program execution.

8. When the actual temperature in the incubator reaches set values, open the door and place samples on the platform.
   Caution! Place the samples symmetrically in relation to the center of the platform to avoid imbalance errors.
   Caution! Do not fill the vessels inside the incubator. Please make sure that all vessels are tightly sealed. Humidity caused by evaporation from unsealed vessels inside the incubator will damage the unit!

9. Press the Run Stop key. The platform will start rotating and the timer indicator will start counting the time interval.
   Note. If the speed is set to zero, pressing the Run Stop key will start the timer, but the platform will not move.

10. After finishing the program (after the set time elapses), the platform motion stops and the status bar shows STOP accompanied by the repetitive sound signal. Press any key to stop the signal.
    Caution! The chamber heating can only be stopped manually by reducing the temperature.

11. If the time is not set (set time is 00:00), the Run Stop key will start continuous operation of the unit until the Run Stop key is pressed again.

12. The platform motion can be stopped at any time by pressing the Run Stop key. In this case, the program realisation and the platform motion will stop and the timer will switch into the STOP mode saving previously set time. Press the Run Stop key to restart the operation with the same time and speed. Additionally, if the door is opened the platform will stop; after closing the door, platform will reinitiate motion automatically.

13. Incubator menu. The Menu button opens incubator’s menu. Use ▲ and ▼ buttons to navigate and Enter button to select. While browsing in the menu, select the topmost Quit option in order to return from sub-menu to menu.

14. Incubator ID. Option GetID shows the serial number and name of the incubator and its Bluetooth module MAC address, as well as remote PC Bluetooth module MAC address.

15. Date & Time. To set date and time in this submenu, press Enter button to select the parameter, then ▲ and ▼ buttons to change the value and Enter button to confirm changes.

16. Service. This submenu has following options: Current Errors and Settings.

17. Current Errors. This option allows user to view and reset the list of active errors.

18. Settings. This option has two suboptions available – Alarm Setting and Start mode.

    * Alarm Setting. User can set the alarm settings based on values of temperature and RPM. All alarms are working on the following principle, temperature taken as an example:  

If Tact < Tthr.low or Tact > Tthr.high for tdelay minutes, then an alarm will be triggered. Where Tact is the actual temperature inside the incubator, Tthr.low is the lower threshold limit value, Tthr.high is the higher threshold limit value and tdelay is the time delay in minutes. Time delay is used in order to avoid false triggering of the alarms. RPM alarm is operated using the same principle.

    * Start Mode. User can change the acceleration mode of the unit. In Start Mode Slow the unit picks up speed gently to reduce the possibility of spilling. In Start Mode Fast the unit rapidly gains speed and set RPM rate.
4. Active Alarms. This option allows user to view and reset the list of active alarms.
5. Data Logger. Data logger features following options: View Plot T(t), View Plot RPM(t), View Plot T(t)+RPM(t), Clear log, Logger on/off.  

All View Plot options use the same interface. Use Left and Right buttons to move the plot along the x axis. Use Up and Down buttons to move the plot along the y axis.
Use <-xx-> button to choose the x axis navigational scale. Available scales are 15 minutes, 1 hour, 1 day, 1 week and 1 month.
Clear Log button clears the logger’s data.
Logger On/Off button toggles the automatic logging of the data.
Available memory indicator. When the data logger uses up all available memory, it begins overwriting data from beginning. 100% of memory is used up in approximately 1 month.

6. At the end of operation, set the Power switch in position O (off). Disconnect the power cable from electric circuit.

Specifications

The unit is designed for operation in cold rooms and closed laboratory rooms at ambient temperature from +4°C to +40°C in a non-condensing atmosphere and maximum relative humidity 80% for temperatures up to 31°C decreasing linearly to 50% relative humidity at 40°C.

Biosan is committed to a continuous programme of improvement and reserves the right to alter design and specifications of the equipment without additional notice.

Temperature specification

Setting range: +25°C  +80°C (increment 0.1°C)
Control range: 5°C above ambient: +80°C
Uniformity1: ±0.3°C
Accuracy1: ±0.1°C
Stability1: ±0.1°C
Heatuptimeto+80°C inside the chamber : 75 min

General specification
Speed control range: 50 – 400 RPM (increment 10 RPM)
Digital time setting: 1 min – 96 hrs (increment 1 min) / non-stop
Maximum continuous operation time2: 30 days
Maximum load: 10.6 kg
Orbit:: 20 mm
Display: TFT LCD
Data transfer: Bluetooth
Dimensions: 620x620x510 mm
Dimensions of the inner chamber: 460x350x400 mm
Stacking: up to 3 units3
Operating voltage/ power consumption: 230 V, 50/60 Hz / 500 W (2.2 A)
Weight4: 48 kg

Table 1. Recommended maximum speed for UP-168 platform with fully loaded TR-21/50 or TR-44/15, depending on the set angle.

Data for samples, at 37°C and 240 RPM

1. Recommended interval between prolonged operations – not less than 8 hours
2. Only with the stacking kit installed. Base side-mounted leg parts are required for third level stacking
3. Accurate within ±10%

Ordering information

Models and versions available

1. To inquire about or order the optional accessories, contact Biosan or your local Bio- san representative.
2. Optional accessories for ES-20/80 :
   Model| Description
   ---|---
   HSP-30/100| Platform with clamps for 30 flasks of 100 ml
   HSP-16/250| Platform with clamps for 16 flasks of 250 ml
   HSP-9/500| Platform with clamps for 9 flasks of 500 ml
   HSP-6/1000| Platform with clamps for 6 flasks of 1000 ml
   PP-400| Flat platform with non-slip silicone mat. Working dimensions 360×400 mm
   UP-168| Universal platform for different flasks
   HSC-50| Clamp for 50 ml flask for UP-168 platform (ø 50 mm)
   HSC-100| Clamp for 100 ml flask for UP-168 platform (ø 65 mm)
   HSC-250| Clamp for 250 ml flask for UP-168 platform (ø 85 mm)
   HSC-500| Clamp for 500 ml flask for UP-168 platform (ø 105 mm)
   HSC-1000| Clamp for 1000 ml flask for UP-168 platform (ø 130 mm)
   TR-21/50| Variable angle test tube rack for 21 tubes of 50 ml for UP-168 platform
   TR-44/15| Variable angle test tube rack for 44 tubes of 15 ml for UP-168 platform
    | Bluetooth adapter
    | Stacking kit for two units
    | Stacking kit for three units

Care and maintenance

1. If the unit requires maintenance, disconnect the unit from the mains and contact Biosan or your local Biosan representative.

2. All maintenance and repair operations must be performed only by qualified and specially trained personnel.

3. Cleaning and desinfection.
   Standard ethanol (75%) or other cleaning agents recommended for cleaning of laboratory equipment can be used for cleaning and decontamination of the steel surfaces.

4. For decontamination, it is recommended to use a special DNA/RNA removing solution (e.g. PDS-250, DNA-Exitus Plus™, RNase-Exitus Plus™).

5. Fuse replacement. Disconnect the power cable from the mains.
   Disconnect the power cable from the socket on the rear of the unit.
   Open the fuse holder, located near the socket. Check and replace with a correct fuse if necessary, see table below:
   Model & version| Fuse 1
   ---|---
   ES-20/80 V.1CD (230 V)| M 4 A

Warranty

1. The Manufacturer guarantees the compliance of the unit with the requirements of Specifications, provided the Customer follows the operation, storage and transportation instructions.
2. The warranted service life of the unit from the date of its delivery to the Customer is 24 months. For extended warranty, contact Biosan or your local Biosan representative.
3. Warranty covers only the units transported in the original package.
4. If any manufacturing defects are discovered by the Customer, an unsatisfactory equipment report shall be compiled, certified and sent to Biosan or your local Biosan representative.
   The following information will be required in the event that warranty or post- warranty service comes necessary. Complete the table below and retain for your records.Model| Serial number| Date of sale
   ---|---|---
   ES-20/80 , Orbital shaker-incubator|  |
5. Fuse type M – time lag Medium

EU Declaration of conformity

Biosan SIA
Ratsupites 7 k-2, Riga, LV-1067, Latvia
Phone: +371 67426137
Fax: +371 67428101
Edition 1.03 – November of 2020

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