levenhuk MED 45B Binocular Microscopes User Manual

: August 10, 2024
: levenhuk

Table of Contents

levenhuk MED 45B Binocular Microscopes
General Information
Microscope assembly
Microscope use: The bright-field method
Phase-contrast device
Assembly and using
Specifications
Care and maintenance
Levenhuk International Lifetime Warranty
References
Read User Manual Online (PDF format)
Download This Manual (PDF format)

levenhuk MED 45B Binocular Microscopes

User Manual
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Eyepiece
Binocular/trinocular eyepiece head
Arm
Revolving nosepiece
Objective
Stage
Mechanical scale
Brightness adjustment knob (not shown)
Aperture diaphragm adjustment screws
Coarse focusing knob
Fine focusing knob
Condenser with filter holder
Collector
Stage moving knob (right/left)
Stage moving knob (back/forth)
Illumination on/off button
Beam splitter (trinocular models only)
Diopter adjustment ring
Binocular/trinocular head locking screw
Third eyepiece tube (trinocular models only)
Digital camera
USB cable
Installation CD
Digital camera with LCD screen
AC adapter
Phase condenser with annular diaphragms
Revolving disk
Adjustment screws
Iris diaphragm
Iris diaphragm control
Auxiliary microscope
Auxiliary microscope eyepiece
Infinity Plan Phase contrast objectives, designed for a cover glass thickness of 0.17mm

Levenhuk MED 45 Microscopes

General Information

Read the user manual carefully before you start working with a microscope. Levenhuk MED Series biological microscopes are safe for health, life and property of the consumer and the environment when properly used, and meets the requirements of international standards. These microscopes are designed for observing transparent objects in the transmitted light using the phase contrast as well as the bright and dark field method. They perform well in clinical research and tests; teaching demonstrations; bacterioscopy and cytology in medical and health establishments, laboratories, universities; and may be used for scientific research in agriculture and microbiology.

Microscope assembly

Unpack the microscope carefully and place it on a flat surface.
Remove the plastic bags and dustproof cover of the eyepiece head.
Install the binocular or trinocular head and tighten the locking screw.
Inspect all the items included in the kit and define their purpose.
Connect the power cord to the microscope and plug it in.

Microscope use: The bright-field method

Please refer to the fig. 1а (binocular model) and fig. 1b (trinocular model).

Turn on the power and the illumination will turn on. Set the brightness at approximately 70%. Place the specimen on the stage. Make sure the clips hold the specimen firmly in place.
Chose the “BF” (bright field) position on the revolving disk of the phase contrast condenser.
Iris diaphragm should be adjusted in accordance with numerical aperture of a lens. The best practice is to make the iris diaphragm slightly smaller than the aperture of the selected lens. Open or close the iris diaphragm using the adjustment screws. If the adjustment screw is turned to the right, the iris diaphragm is fully open. Note: The diaphragm is not intended for adjusting the brightness of the illumination. To adjust the brightness, use the brightness adjustment knob.
Slide the filter holder, place the filter in it and then return the holder to its initial position.
To change the objective magnification, turn the revolving nosepiece until it clicks.
Make sure that the lens does not touch the sample when adjusting the focus: Rotate the coarse focus knob until the specimen is about 3.175mm (1/8″) away from the lens.
Looking into the eyepiece, slowly turn the coarse focusing knob until you can see the image of the observed specimen. Turn the fine focusing knob to make the image clear. The fine focusing mechanism allows you to focus on the observed specimen while using high magnifications.
When you use an oil immersion objective, the space between the specimen and the lens should be filled with immersion oil. Raise the condenser to the upper position and put a drop of oil on the lens and on the cover glass of the specimen. Move the revolving nosepiece back and forth to get rid of air bubbles in the oil. Then, fix the objective lens in its working position. Make sure that oil fills the entire space between the objective lens and the specimen. After use, wipe the lens dry.
To adjust the position of the mechanical scale, turn the coarse focusing knob in a clockwise direction if you want to fix the position of the mechanical scale or in a counterclockwise direction if you want to loosen it.
To place the specimen in the field of view, move the stage horizontally back and forth or left and right using the stage moving knobs.
Look through the right eyepiece with your right eye and adjust the image sharpness with the coarse and fine focusing knobs. Then, look through the left eyepiece with your left eye and rotate the diopter adjustment ring to equalize the difference between your left and right eyes vision. Remember the setting for future use. Adjust the distance between the eyepiece tubes of the binocular head so that the image merges into a single circle.
Trinocular models: You can install the digital camera to the third eyepiece tube. Use the beam splitter to send the light to the eyepieces or to the camera.

Phase-contrast device

Configuration and operating principle
Please refer to fig. 3. The device is designed for studying low-contrast objects that are not visible in the microscope when observed in transmitted light in a bright field. It allows for observing unstained low-contrast specimens and living microorganisms using the phase contrast method.

The light that passes through an unstained specimen changes the light wave phase, but these changes are invisible to the human eye. To make such a specimen visible, it is necessary to convert the phase shifts of a light into changes in amplitude, which can be observed. The phase contrast device (consisting of a phase condenser and phase objectives) makes the image of a specimen contrast enough and suitable for research.
The phase condenser enables observing using the dark and bright field methods. The iris diaphragm under the revolving disk is used for bright-field research, and in the disk itself there is a hole for the passage of the entire light beam.

Assembly and using

Install the phase objectives into the revolving nosepiece and the auxiliary microscope into the microscope tube.
Install the phase condenser into a ring-shaped mount under the stage.
Fully open the iris diaphragm of the phase condenser.
Rotate the revolving disk, select the desired annular diaphragm; the corresponding number will appear in the window of the condenser (the magnification of the lens should coincide with the marking of the aperture of the phase-contrast device). The “BF” position is intended for using the bright-field method, and the “DF” position is intended for research in a dark field.
In the eyepiece of the auxiliary microscope, besides the phase ring, the diaphragm ring can be seen.
Center the image of the condenser annular diaphragm in respect to the objective phase ring using the adjustment screws and then focus on the objective phase ring.
Fully align the dark ring with the light one. If necessary, move the condenser upwards to align and center the rings with each other and get the most contrast image. Use filters to make the image even more contrast.
Replace the auxiliary microscope with an eyepiece and start researching.
Note : Each change of a specimen or objective lens requires checking and/or centering of the annular diaphragm regarding the phase ring.

Fuse replacement
Unplug the microscope from a power source. Open the fuse holder located on the back of the microscope body with a flat head screwdriver. Remove the old fuse and install a new one. Only use the appropriate types of fuses. Close the fuse holder.

Digital camera
Levenhuk MED D45T comes with a digital camera (fig. 2a), Levenhuk MED D45T LCD comes with a digital camera with an LCD screen (fig. 2b). A digital camera is installed to the third eyepiece tube and enables observing specimens on the screen in exquisite detail and true color, taking photos and videos, and saving the images for future reference.

Specifications

Product ID	74008	74009	74010	74011
Model	MED 45B	MED 45T	MED D45T	MED D45T LCD
Type	biological/optical	biological/optical, digital
Research method	phase contrast, bright and dark field	phase contrast, bright

and dark field
Magnification| 40—1000x| 40—1000x
Interpupillary distance| 48—75mm| 48—75mm
Eyepiece head| binocular, 360° rotatable, inclined at 30°| trinocular, 360° rotatable, inclined at 30| trinocular, 360° rotatable, inclined at 30°
Optics material| optical glass with an anti-fungal coating| optical glass with an anti-fungal coating
Eyepiece tube diameter| 30mm| 30mm
Third vertical eyepiece tube| —| 23.2mm| 23.2mm
Eyepieces| WF10x/22mm (2pcs)| WF10x/22mm (2pcs)
Eyepiece diopter adjustment| ±5D| ±5D
Objectives| Infinity Plan Phase contrast: 4x, 10x, 40xs, 100xs (oil)| Infinity Plan Phase contrast: 4x, 10x, 40xs, 100xs (oil)
Revolving nosepiece| 5 objectives| 5 objectives
Stage moving range| 75/50mm| 75/50mm
Stage| double layer mechanical, 180x150mm, with a mechanical scale| double layer mechanical, 180x150mm, with a mechanical scale
**** Focus system| coaxial, coarse (0.5mm) and fine (0.002mm), with rack and pinion mechanism| coaxial, coarse (0.5mm) and fine (0.002mm), with rack and pinion mechanism
Condense| phase (dark field) with iris diaphragm, with dovetail mount| phase (dark field) with iris diaphragm, with dovetail mount
Body| metal| metal
Illumination| lower (5W LED) with brightness adjustment| lower (5W LED) with brightness adjustment
Collector| Köhler illumination| Köhler illumination
Filters| blue, green, yellow| blue, green, yellow
Vial of immersion oil| +| +
---|---|---
Fuse| 2pcs| 2pcs
Power supply| 100—240V, via AC adapter| 100—240V, via AC adapter
Digital camera| —| 18Mpx| 5Mpx with LCD screen
Camera power supply| —| DC 5V, via USB 3.0 cable| DC 12V/2A, via AC adapter
Optional accessories (sold separately)| eyepieces (D 30mm): 5х, 12.5х, 16x, 20x| eyepieces (D 30mm): 5х, 12.5х, 16x, 20x
—| digital camera| —

Caution! Please remember that mains voltage in most European countries is 220—240V. If you want to use your device in a country with a different mains voltage standard, remember that use of a converter is absolutely necessary. The microscope must be grounded. Make sure that the main voltage matches the voltage indicated on the microscope body.

Care and maintenance

Never, under any circumstances, look directly at the Sun, another bright source of light or at a laser through this device, as this may cause PERMANENT RETINAL DAMAGE and may lead to BLINDNESS.
Take necessary precautions when using the device with children or others who have not read or who do not fully understand these instructions.
After unpacking your microscope and before using it for the first time check for integrity and durability of every component and connection.
Do not try to disassemble the device on your own for any reason. For repairs and cleaning of any kind, please contact your local specialized service center.
Protect the device from sudden impact and excessive mechanical force. Do not apply excessive pressure when adjusting focus. Do not overtighten the locking screws.
Do not touch the optical surfaces with your fingers. To clean the device exterior, use only special cleaning wipes and special optics cleaning tools from Levenhuk. Do not use any corrosive or acetone-based fluids to clean the optics.
Abrasive particles, such as sand, should not be wiped off lenses, but instead blown off or brushed away with a soft brush.
Do not use the device for lengthy periods of time, or leave it unattended in direct sunlight. Keep the device away from water and high humidity.
Be careful during your observations, always replace the dust cover after you are finished with observations to protect the device from dust and stains.
If you are not using your microscope for extended periods of time, store the objective lenses and eyepieces separately from the microscope.
Store the device in a dry, cool place away from hazardous acids and other chemicals, away from heaters, open fire and other sources of high temperatures.
When using the microscope, try not to use it near flammable materials or substances (benzene, paper, cardboard, plastic, etc.), as the base may heat up during use, and might become a fire hazard.
Always unplug the microscope from a power source before opening the base or changing the illumination lamp. Regardless of the lamp type (halogen or incandescent), give it some time to cool down before trying to change it, and always change it to a lamp of the same type.
Always use the power supply with the proper voltage, i.e. indicated in the specifications of your new microscope. Plugging the instrument into a different power outlet may damage the electric circuitry of the microscope, burn out the lamp, or even cause a short circuit.
Seek medical advice immediately if a small part or a battery is swallowed.

Levenhuk International Lifetime Warranty

All Levenhuk telescopes, microscopes, binoculars and other optical products, except for accessories, carry a lifetime warranty against defects in materials and workmanship. Lifetime warranty is a guarantee on the lifetime of the product on the market. All Levenhuk accessories are warranted to be free of defects in materials and workmanship for six months from date of retail purchase. Levenhuk will repair or replace such product or part thereof which, upon inspection by Levenhuk, is found to be defective in materials or workmanship. As a condition to the obligation of Levenhuk to repair or replace such product, the product must be returned to Levenhuk together with proof of purchase satisfactory to Levenhuk.
This warranty does not cover consumable parts, such as bulbs (electrical, LED, halogen, energy-saving and other types of lamps), batteries (rechargeable and non-rechargeable), electrical consumables etc.
For further details, please visit our web site: www.levenhuk.com/warranty
If warranty problems arise, or if you need assistance in using your product, contact the local Levenhuk branch.

The original Levenhuk cleaning accessories

Levenhuk Cleaning Pen LP10

Removes dust with a brush
The soft tip is treated with a special cleaning fluid that removes greasy stains Does not damage optical coatings of the lenses
Leaves no smudges or stains

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USA: www.levenhuk.com
United Kingdom: www.levenhuk.uk

USA : www.levenhuk.com

Levenhuk USA: 928 E 124th Ave. Ste D, Tampa, FL 33612, USA, +1 813 468-3001, contact_us@levenhuk.com
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