Thermo Scientific Thermal Mixer with Blocks User Manual

Thermo Scientific Thermal Mixer with Blocks User Manual

Introduction

This Thermo Scientific Thermal Mixer provides mixing and temperature control of samples in micro test tuoes or PCR plates. Features of thermal mixers meet the highest expectations of users according to many parameters:

• Fast reaching of specified mixing speed and maintenance of equal amplitude of rotation throughout the thermal mixer block;
• Stability of maintaining the set temperature in a wide range throughout the olock surface of thermal mixers;
• With the help of the temperature calibration function, the user can calibrate the unit approximately +6% of the selected temperature to compensate differences in the thermal oehaviour of tubes from different manufacturers;
• LCD display indicates pre-set and current values of temperature, speed and time of operation;
• Ouiet motor operation, compact size, prolonged service life;
• Sensor error handling and diagnostics;

Functions of heating and mixing can be performed either simultaneously or independently, that allows using the unitas three independent devices:

• Thermostat;
• Shaker;
• Thermo shaker.

We offer five heating and cooling oloc<s for each model, including a olock with a plastic lid for PCR-plates.
Within one model of thermal mixer, the blocks are mutually interchangeable and can be easily installed.

The devices are applicable in:

• Genetic analyses: in extraction of DNA, RNA and funher sample preparation;
• Biochemistry:  for studying of enzymatic reactions and processes;
• Cellular biology:   extraction of metabolites from cellular material.

Safety Information

Caution: Make sure you have fully read and understood the present Manual before using the equipment.
Please pay special attention to sections marked by this symbol.
Caution: Hot surface! Platform surface becomes very hot during use.
Always use protective cotton gloves to install or remove samples when the temperature is set higher than 60°C.

General safety

• Use only as specified in the operating manual provided.
• Save the unit from shocks or falling.
• Store and transport the unit in a horizontal position (see package label) at ambient temperatures between -20°C and +60°C and maximum relative humidity of 80%.
• After transportation or storage keep the unit under room temperature for 2-3 h before connecting it to the electric circuit.
• Use only original parts and accessories, provided by Thermo Fisher Scientific for this product.
• Before using any cleaning or decontamination methods except those recommended by the Thermo Fisher Scientific, check with the Thermo Fisher Scientific that the proposed method will not damage the equipment.
• Do not make modifications to the design of the unit.

Electrical safety

• Connect only to the external power supply with voltage corresponding to that on the serial number label
• Use only the external power supply provided with this product.
• Ensure that the power switch and external power supply are accessible during use.
• Do not plug the unit into an ungrounded power socket, and do not use an ungrounded extension lead.
• Disconnect the unit from electric circuit before moving.
• If liquid penetrates into the unit, disconnect it from the external power supply and have it checked by a repair and maintenance technician.
• Do not operate the unit in premises where condensation can form.
  Operating conditions of the unit are defined in the Specifications section.

During operation

• Do not leave the operating unit unattended.

• Do not impede the platform motion.

• Do not operate the unit in environments with aggressive or explosive chemical mixtures.
  Please contact Thermo Fisher Scientific for possible operation of the unit in specific atmospheres.

• Do not operate the unit if it is faulty or has been installed incorrectly.

• Do not use outside laboratory rooms.

• Do not check the temperature by touch. Use a thermometer.

Biological safety

• It is the user’s responsibility to carry out appropriate decontamination if hazardous material is spilt on or penetrates into the equipment.

Getting Started

Unpacking
Remove packing materials carefully and retain for future shipment or storage of the unit.
Examine the unit carefully for any damage incurred during transit.
The warranty does not cover in-transit damage.
Warranty covers only units transported in the original package.

Complete Sets

Blocks, Optional or included in the set:

Setup

Place the unit upon even horizontal staole non-flammable surface 30 cm away from any flammable materials, and clear 20 cm around the device on all sides for ventilation.
Remove protective film from the display.
Plug the external power supply into the socket at the rear side of the unit.
Connect the power cable to the external power supply.

Thermo block installation

If a thermo block is not installed, choose the thermo block, connect the plug to the contact terminal according to the
scheme on fig. 1/1 on the underside of the thermo block.
Make sure that the connector is mounted tightly.

Caution: Thermo block installation and replacement have to be performed only when the Power switch is turned off and external power supply is disconnect from the device.

Align the thermo block so that the warning label & is facing the front of the unit (fig. 2). Secure with the four knurled screws (fig. 2/1) or four hex screws.

Changing blocks

Disconnect the external power supply from the device.
Remove the four knurled screws or four hex screws (in microplate thermo blocks). Lift the block without damaging the cable and disconnect the plug (fig. 1/1). Select the new thermo block and install it according to the previous paragraph.
**Figure 1. Thermoblock connection

Figure 2. Thermoblock setup

**

Operation

Recommendations during operation

• Please check the tubes/microplates before using, be sure that tubes and micro plates are heat-resistant.
• Do not heat the microplates over the melting point of the material they are made of.
• We recommend filling tubes and plate wells up to 75% of rated volume for efficiency.
  Caution: Platform surface becomes very hot during use.
  Please, take necessary care and use protective cotton gloves to install or remove test samples when set temperature is higher than 60°C.
  

Caution: Heating/temperature maintenance process does not stop when the timer is finished.
Platform thermal regulation can be turned off only by setting the required temperature below 25 °C (the display will show OFF – Temp – set point). In this mode, thermal mixer can be used in the cold rooms as a mixing device without thermoregulation.

Caution: Do not fill microtubes or microplates directly inside the unit.
Note: If the rotation speed is set to zero, pressing rpm Run/Stop key will start the timer but the platform will not move.

Starting the unit
Connect external power supply to a grounded power socket and set the power switch, located on the rear panel of the unit, to position I (on).
The display will turn on with the upper line (Set) showing time, speed and temperature set earlier and the lower line (Actual) showing current status: STOP indication, 000 rpm speed and platform temperature in °C.
If a temperature is set, then the platform temperature that automatically rises to that temperature.
The time of temperature stabilization depends on the room temperature.
If the heating of is turned off by setting the temperature below or 4°C, top line shows indication OFF.

Setting the parameters
Use the readings in the upper line of the display (Set), while setting the required parameters.
Pressing the key for more than 3 s will increase the increment rate.
Speed and temperature can be changed during operation.
Using the   and Time keys (fig. 3/1) set the required working time interval in hours and minutes (step 1 min).
Using the and Speed keys (fig. 3/2) set the required speed (step 10 rpm).
Using the and Temp keys (fig. 3/3) set the necessary temperature (step 0.1°C).

Program execution
After the thermal stabilization of the thermal mixer, i.e. when the set and current temperature readings become the same:
Place samples on the platform. Press the rpm Run/Stop key (fig. 3/4).
The platform will start rotating and the timer indicator will start counting the time interval (with 1 min precision).
After finishing the program (after the set time elapses) the platform motion will stop and the timer will show the flashing reading STOP accompanied by the repetitive sound signal until the rpm Run/Stop key is pressed.
If the working time is not set (or is reset) and the timer indicator in the upper line shows 00:00, pressing the rpm Run/Stop key will start continuous operation of the device with countdown timer in the lower line (Actual) until the rpm Run/Stop key is pressed again.

Caution: At the end of the set time period the platform movement is stopped automatically, but the heating can  stopped only manually ay reducing the temperature using the ▼ Temp key (fig. 3/3 — lower key) till the OFF sign appears in the upper line (Set) of the display
Caution: The platform remains hot after use. Please, take necessary care and use protective cotton gloves to install or remove test samples when set temperature is higher than 60°C.

If required, there is possibility to restart the timer when it is running.
Press the Time Run/Stop key once (fig. 3/5) to stop the timer.
Press the Time Run/Stop key again to restart the timer.
The platform motion can oe stopped at any time by pressing the rpm Run/Stop key.
In this case, the program realization and the platform motion will stop and the timer will switch into the STOP mode saving previously set time.
Press the rpm Run/Stop key to repeat the operation with the same time and speed.
Finishing the operation Set the Power switch, located on the rear panel of the unit, in position O (O/ and disconnect the external power supply from electric circuit.

Calibration

Note: Values marked in grey on figures 4 and 5 are not used in calibration and are meant for service engineers.

Figure 4: Display in calibration mode:

1. Calibration mode indicator;
2. Calibration coefficient;
3. Temperature with current coefficient

Note: Coefficient value changes are recommended after the unit has reached 30°C temperature.
Note: Calibration coefficient can be changed in range from 0.936 to 1.063 (±0.063), with increment of 0.001.
This calibrating coefficient will correct temperature through all the operation range.

Figure 5: Changing the coefficient:

1. Calibration coefficient;
2. Temperature with current coefficient

Figure 6: Display after calibration:

1. Set temperature;
2. Current calibrated temperature
   

The device is recalibrated at the factory (calibrating coefficient is 1.000) for operation with temperatures measured by a sensor in the heating block.
To change the calibration coefficient, hold the Time Run/Stop key pressed for more than 8 s to activate calibration mode.
The calibration coefficient appears on the display (figure 4).

Restoring factory settings
Set 1.000 value using the and Temp keys as shown on fig. 4/1 to restore the factory settings. Press the rpm Run/Stop key once to save the changes and exit the calibration mode.

Calibration procedure
To calibrate the unit, use an independent sensor with 0.5°C accuracy, which can fit in the cell of a microplate on the platform.
Install the sensor into a cell of the microplate.
Set the required temperature in operation mode, e.g. 40°C.
After the unit reaches the set temperature (when the set and current temperature readings equal), leave the unit for 30 min for thermal stabilization.
Let us assume that the readings of independent sensor is 39°C, but the display’s actual temperature is 40°C.
Then, it is necessary to add 1°C correction.
Hold the Time RuNStop key pressed for more than 8 s to activate calibration mode (figure 4).
Using the and Temp keys, change the calibration coefficient (fig. 5/1) so that the new temperature value (fig. 5/2) corresponds to the independent sensor temperature.
In our example, the calibration coefficient will be 0.974.
Press the rpm Run Stop key once to save the changes and exit the calibration.
The display will show calibrated temperature as shown on fig. 6/1 and the unit will continue thermal stabilization according to the previously set temperature.

Specifications

The unit is designed for operation in cold rooms, incubators (excluding CO incubators) and closed laboratory rooms at ambient temperature from +4°C to +40°C in a non-condensing atmosphere and maximum relative humidity 80% for temperatures up to 31°C decreasing linearly to 50% relative humidity at 40°C.
Thermo Fisher Scientific is committed to a continuous program of improvement and reserves the right to alter design and specifications of the equipment without additional notice.

 |
---|---
Setting range| +4°C to +100°C
Lower contro ranae| 15°C be ow RT1
Upper control range| +100°C
Setting resolution| 0.1°C
Stability, at +37°C| ±0.1°C
Maintaining accuracy2, at +37°C| ±0.5°C
Uniformity over the platform,|
at +4°C| ±0.6°C
at +37°C| ±0.1°C
at +100°C| ±0.3°C
Average heating speed from +25°C to +100°C| 5°C/min
Average cooling speed from +100°C to +25°C| 5°C/min
from +25°C to +4°C| 1.8°C/min
Calibration option| yes
Calibration coefficient range| 0.936…1.063

Maintenance

Service
If the unit requires service maintenance, disconnect the unit from the electric circuit and contact Thermo Fisher Scientific.
All maintenance and repair operations must be performed only ay qualified and specially trained personnel.

Cleaning
Standard ethanol (75%) or other cleaning agents recommended for cleaning of laooratory equipment can be used for cleaning and decontamination of the unit.

Rubber belt replacement
For maintenance of reliable operation of the device, the Thermo Fisher Scientific recommends to replace ruooer
belts after 1.5 years or 2000 hours of operation time.
Disconnect the external power supply from the device.
Remove 4 fixation screws on the device oottom and remove the bottom plate.
Replace the rubber oelt (fig. 7).

Reassemble the device.

Error codes in case of a defect
Some malfunctions trigger an error code to appear on display, accompanied ay a sound signal every 8 s. Press rpm Run/Stop key to turn off the signal. Error code format is letters ER and a single digit.
Disconnect the unit from the electric circuit and repon the error code to Thermo Fisher Scientific.

Warranty

The following information will be required in the event that warranty or post- warranty service comes necessary.
Complete the taole below and retain for your records.

Thermo Scientific Thermal Mixer

• Serial number
• Date of sale
• Purchase or Sales
• Order Numoer

Thermo Fisher Scientific guarantees the compliance of unit with the requirements of specifications, if the customer follows operation, storage and transportation instructions.
The warranted service life of unit from date of delivery to the customer is 24 months, excluding blocks and replacement pans mentioned in Taoles 1 & 2. For extended warranty, contact Thermo Fisher Scientific. Warranty covers only the units transported in the original package.
If any manufacturing defects are discovered by the Customer, an unsatisfactory equipment repon shall be compiled, cenified and sent to Thermo Fisher Scientific.

EU Declaration of Conformity

This declaration of conformity indicates that the equipment meets the require- ments of the following Directive(s):

EMC Directive 2014/30/EU
Low Voltage Directive 2014/35/EU
RoHS2 2011/65/EU1
WEEE 2012/19/EU

Applied standards:

• EN 61326-1: Electrical equipment for measurement, control and laboratory use EMC requirements. General requirements.

• EN 61010—1: Safety requirements for electrical equipment for measurement, control and laboratory
  use. General requirements.

• EN 61010—2—010: Particular requirements for laboratory equipment for the heating of materials.

• EN 61010—2—051: Particular requirements for laboratory equipment for mixing and stirring.

Support

Find out more at thermofisher.com
For Research Use Only. Not for use in diagnostic procedures. @ 2019 Thermo Fisher Scientific
Inc. All rights reserved. All trademarks ¿tre the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.

References

• Thermo Fisher Scientific - AU
• Thermo Fisher Scientific - US
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