Thermo Scientific Evolution One Series UV-Vis Spectrophotometers User Guide

Evolution One Series UV-Vis Spectrophotometers
Evolution One Series – Getting Started
User Guide

Evolution One Series UV-Vis Spectrophotometers

This guide gives an overview of common tasks that can be performed with the spectrophotometer. For more information, see the Evolution One Series User Guide or Insight Pro Help.

Spectrophotometer Components

Computer Control

To set up a Computer Control instrument

1. Turn on the computer.

2. Install Insight Pro™ software.

3. Connect the instrument to the computer using a USB cable.

4. Turn on the power switch on the back of the spectrophotometer.
   Wait for the system to initialize. The power indicator on the front panel stops blinking when initialization is complete.

5. Double-click the Insight Pro desktop icon to start the software.

Insight Pro Window

The Insight Pro – Classic view displays view options and custom groups along the top row, Application buttons in the center, and various functions along the bottom ribbon.  Classic view is the default view of the home page. Bio Methods view is also available.

Bottom Ribbon
The bottom ribbon provides access to the following:

lists.
| Perform wavelength and accessory calibrations, as well as beam alignment.
| The Settings feature on the Insight Pro page includes Applications, Reports, Preferences, Data, and Formulas & Units.
| Launches Thermo Fisher online help.

Applications

Fixed
Measures light passing through the sample at one or more wavelengths. Click the Fixed button in Insight Pro – Classic view.
Scan
Measures light that passes through the sample over a range of wavelengths. Click the Scan button in Insight Pro – Classic view.
Quant
Use this to set up and perform quantitative analyses of sample data.
Click the Quant button in Insight Pro – Classic view.
Live Display
Used for quick measurements and simplified data collections in Fixed or Scan mode. Click Live Displa y in Insight Pro – Classic view.
Kinetics
Use this to make time- or temperature-based kinetics measurements.
Click the Kinetics button in Classic view.
Note Bio methods are displayed in Bio Methods view.

Common Tasks

Start the Insight Pro software, display the Home screen, and select the Classic group before beginning the first task.
Data Acquisition
To measure samples

1. Click an application button, such as Scan. This will open the setting page for that Application.
2. Set the desired parameters for the experiment.
3. Click .
4. Follow the instructions that appear, providing information or installing a blank, sample, or standard. The acquired data will appear:To create a report containing the data, use the next procedure.

Reports
An application that contains measurement results must be selected before you can create a report.
To create and print a report

1. Click .
2. Use the Reportable Data tab to specify the measurement results to include in the report.
3. Use the Layout tab to design the report.
4. Use the Samples tab to select the sample data to include.
5. Click .

Data Storage
To open saved data and the associated application

1. Click from the Bottom Ribbon of the Insight Pro window.
2. Double-click the desired workbook file from the list.

Instrument Performance
To verify instrument performance

1. Click from the Bottom Ribbon of the Insight Pro window.

2. Select the tests to run. Some tests require reference materials.
   If using an accessory, see its documentation for instructions.

3. Click .

4. Follow the instructions that appear.
   Test results are saved and appear in a window from which they can be printed or copied. They can also be accessed later on the PV Reports tab in History.
   All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.

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