Thermo Scientific Multidrop 384 Liquid Dispensing User Manual

Multidrop 384 Liquid Dispensing

Thermo Scientific Multidrop® 384
User Manual
Rev. 3.4

Thermo Scientific Multidrop® 384
User Manual
Rev. 3.4, Cat. no. 1507010

Thermo Scientific Multidrop 384, Cat. no. 5840150 or 5840157 User Manual Rev. 3.4; October 2008, Cat. no. 1507010
CONTENTS
1 SAFETY SYMBOLS AND MARKINGS………………………………………………………………………………. 6 2 ABOUT THE USER MANUAL ………………………………………………………………………………………….. 7 3 INTRODUCTION TO THE MULTIDROP 384………………………………………………………………………. 8 4 FUNCTIONAL DESCRIPTION ………………………………………………………………………………………… 10 5 INSTALLATION…………………………………………………………………………………………………………….. 15 6 ROUTINE OPERATION………………………………………………………………………………………………….. 21 7 MAINTENANCE…………………………………………………………………………………………………………….. 36 8 TECHNICAL SPECIFICATIONS ……………………………………………………………………………………… 60 9 TROUBLESHOOTING……………………………………………………………………………………………………. 66 10 WARRANTY CERTIFICATE …………………………………………………………………………………………… 71 11 ORDERING INFORMATION …………………………………………………………………………………………… 72 12 GLOSSARY AND ABBREVIATIONS ………………………………………………………………………………. 75 13 INDEX…………………………………………………………………………………………………………………………… 77 14 TABLE OF FIGURES …………………………………………………………………………………………………….. 81 APPENDIX 1. THERMO SCIENTIFIC MULTIDROP 384 BRIEF USER’S GUIDE………………………… 83 APPENDIX 2. THERMO SCIENTIFIC MULTIDROP 384 FEEDBACK FORM……………………………… 84 APPENDIX 3. ADDRESSES………………………………………………………………………………………………….. 85

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

2

TABLE OF CONTENTS
1 SAFETY SYMBOLS AND MARKINGS………………………………………………………………………………. 6
2 ABOUT THE USER MANUAL ………………………………………………………………………………………….. 7
3 INTRODUCTION TO THE MULTIDROP 384………………………………………………………………………. 8
3.1 INTENDED USE ……………………………………………………………………………………………………………. 8 3.2 PRINCIPLE OF OPERATION……………………………………………………………………………………………… 8 3.3 ADVANTAGES OF USING THE MULTIDROP 384 ……………………………………………………………………. 9
4 FUNCTIONAL DESCRIPTION ………………………………………………………………………………………… 10
4.1 INSTRUMENT LAYOUT………………………………………………………………………………………………….. 10 4.1.1 Front view ………………………………………………………………………………………………………… 10 4.1.2 Back view…………………………………………………………………………………………………………. 13
4.2 MULTIDROP 384 MICROPLATE DISPENSER……………………………………………………………………….. 14
5 INSTALLATION…………………………………………………………………………………………………………….. 15
5.1 INSTALLATION CHECK LIST……………………………………………………………………………………………. 15 5.2 WHAT TO DO UPON DELIVERY……………………………………………………………………………………….. 15
5.2.1 How to unpack ………………………………………………………………………………………………….. 15 5.2.2 Checking delivery for completeness…………………………………………………………………….. 15 5.2.3 Checking for damage during transport …………………………………………………………………. 16 5.2.4 Environmental requirements……………………………………………………………………………….. 16 5.2.5 Things to avoid …………………………………………………………………………………………………. 16 5.2.6 Technical prerequisites………………………………………………………………………………………. 16 5.3 SETUPS BEFORE YOU PUT THE INSTRUMENT INTO OPERATION ……………………………………………… 17 5.3.1 Installation procedure ………………………………………………………………………………………… 17 5.3.2 How to ensure startup ……………………………………………………………………………………….. 18 5.4 OPERATIONAL CHECK …………………………………………………………………………………………………. 19 5.5 OPERATING PRECAUTIONS AND LIMITATIONS BEFORE OPERATION ………………………………………… 20
6 ROUTINE OPERATION………………………………………………………………………………………………….. 21
6.1 OPERATIONAL CHECK LIST …………………………………………………………………………………………… 21 6.2 SWITCHING ON ………………………………………………………………………………………………………….. 21 6.3 HOW TO INSERT THE PRIMING VESSEL AND THE DISPENSING CASSETTE …………………………………. 22 6.4 HOW TO INSERT AND ADJUST THE PLATE ADAPTER ……………………………………………………………. 25 6.5 HOW TO USE THE CONTROL PANEL ………………………………………………………………………………… 30
6.5.1 Control panel indicators……………………………………………………………………………………… 30 6.5.2 Control panel thumbwheels (digital switches) ……………………………………………………….. 30 6.5.3 Control panel keys…………………………………………………………………………………………….. 31 6.6 HOW TO SELECT THE 96 / 384-WELL PLATE……………………………………………………………………… 31 6.7 HOW TO PRIME………………………………………………………………………………………………………….. 32 6.8 DISPENSING……………………………………………………………………………………………………………… 32 6.8.1 How to dispense automatically ……………………………………………………………………………. 32 6.8.2 How to dispense manually into the specified column(s) …………………………………………. 33 6.8.3 How to dispense multiple volumes ………………………………………………………………………. 33
6.8.3.1 Dispensing tenfold and hundredfold volumes………………………………………………………………….. 33
6.8.4 After use ………………………………………………………………………………………………………….. 34 6.9 SHUTDOWN………………………………………………………………………………………………………………. 35 6.10 GUIDELINES FOR USE………………………………………………………………………………………………. 35
7 MAINTENANCE…………………………………………………………………………………………………………….. 36
7.1 REGULAR AND PREVENTIVE MAINTENANCE………………………………………………………………………. 36 7.1.1 General ……………………………………………………………………………………………………………. 36 7.1.2 Immediate ………………………………………………………………………………………………………… 36
7.2 HOW TO WASH THE DISPENSING CASSETTE ……………………………………………………………………… 37 7.3 HOW TO AUTOCLAVE THE DISPENSING CASSETTE ……………………………………………………………… 37

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

3

7.4 HOW TO REPLACE THE FUSES ………………………………………………………………………………………. 38 7.5 HOW TO RECALIBRATE THE DISPENSING CASSETTE……………………………………………………………. 40
7.5.1 Volumetric calibration ………………………………………………………………………………………… 40 7.5.2 Gravimetric calibration……………………………………………………………………………………….. 42 7.6 HOW TO CHANGE THE TUBING SET…………………………………………………………………………………. 45 7.6.1 Changing the tip band ……………………………………………………………………………………….. 45 7.6.2 Fitting the new tubing set step by step …………………………………………………………………. 46 7.7 DISPOSAL OF MATERIALS …………………………………………………………………………………………….. 57 7.8 DECONTAMINATION PROCEDURE …………………………………………………………………………………… 57 7.9 HOW TO PACK FOR SERVICE…………………………………………………………………………………………. 58 7.10 SERVICE CONTRACTS………………………………………………………………………………………………. 58 7.11 DISPOSAL OF THE INSTRUMENT …………………………………………………………………………………. 58

8 TECHNICAL SPECIFICATIONS ……………………………………………………………………………………… 60

8.1 GENERAL SPECIFICATIONS …………………………………………………………………………………………… 60 8.2 SAFETY SPECIFICATIONS……………………………………………………………………………………………… 61
8.2.1 Live parts …………………………………………………………………………………………………………. 61 8.3 IN CONFORMITY WITH THE REQUIREMENTS ………………………………………………………………………. 62 8.4 REMOTE CONTROL TO MULTIDROP 384 ………………………………………………………………………….. 63
8.4.1 Hardware for the computer port ………………………………………………………………………….. 63 8.4.2 Multidrop 384 PC interface …………………………………………………………………………………. 63

9 TROUBLESHOOTING……………………………………………………………………………………………………. 66

9.1 TROUBLESHOOTING GUIDE…………………………………………………………………………………………… 66 9.2 FAQS ABOUT THE MULTIDROP 384 ……………………………………………………………………………….. 67 9.3 HAZARDS…………………………………………………………………………………………………………………. 68
9.3.1 Electrical ………………………………………………………………………………………………………….. 68 9.3.2 Mechanical……………………………………………………………………………………………………….. 68 9.3.3 Environmental…………………………………………………………………………………………………… 68 9.3.4 Defects and abnormal stresses…………………………………………………………………………… 69 9.4 SERVICE REQUEST PROTOCOL ……………………………………………………………………………………… 69 9.4.1 Decontamination……………………………………………………………………………………………….. 69
9.4.1.1 Certificate of Decontamination ……………………………………………………………………………………… 70

10 WARRANTY CERTIFICATE………………………………………………………………………………………… 71

10.1 WARRANTY CERTIFICATE …………………………………………………………………………………………. 71

11 ORDERING INFORMATION………………………………………………………………………………………… 72

11.1 11.2 11.3 11.4 11.5 11.6 11.7 11.8

INSTRUMENTS ……………………………………………………………………………………………………….. 72 DISPENSING CASSETTES ………………………………………………………………………………………….. 72 SPARE PARTS FOR DISPENSING CASSETTES …………………………………………………………………. 73 PRIMING VESSELS (CONSUMABLES) ……………………………………………………………………………. 73 LIST OF ACCESSORIES……………………………………………………………………………………………… 73 LIST OF PLATES (CONSUMABLES) ……………………………………………………………………………….. 73 LIST OF SPARE PARTS ……………………………………………………………………………………………… 74 LIST OF RECOMMENDED SPARE PARTS ………………………………………………………………………… 74

12 GLOSSARY AND ABBREVIATIONS …………………………………………………………………………… 75

12.1 12.2

KEYWORDS FOR WEB PAGES …………………………………………………………………………………….. 76 LITERATURE ………………………………………………………………………………………………………….. 76

13 INDEX……………………………………………………………………………………………………………………….. 77

14 TABLE OF FIGURES………………………………………………………………………………………………….. 81

APPENDIX 1. THERMO SCIENTIFIC MULTIDROP 384 BRIEF USER’S GUIDE………………………… 83

APPENDIX 2. THERMO SCIENTIFIC MULTIDROP 384 FEEDBACK FORM……………………………… 84

APPENDIX 3. ADDRESSES………………………………………………………………………………………………….. 85

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

4

Copyright
Copyright © 2008 Thermo Fisher Scientific Corporation. All rights reserved. First edition published in 1987. Printed in Finland. Reproduction of the accompanying user documentation in whole or in part is prohibited.
Patents
This product is protected by the following patent: US 4995432.
Trademarks
Immulon, Microfluor and Multidrop are registered trademarks of Thermo Fisher Scientific. Microlite is a trademark of Thermo Fisher Scientific.
All other trademarks and registered trademarks are the property of their respective holders.
Disclaimer
Thermo Fisher Scientific reserves the right to change its products and services at any time to incorporate technological developments. This manual is subject to change without prior notice as part of a continuous product development.
Although this manual has been prepared with every precaution to ensure accuracy, Thermo Fisher Scientific assumes no liability for any errors or omissions, nor for any damages resulting from the application or use of this information. This manual supersedes all previous editions.
No liability for consequential damages
Thermo Fisher Scientific shall not be liable for any damages whatsoever arising out of the use or inability to use this product.
Contact information
Thermo Fisher Scientific Oy P.O. Box 100, FI-01621 Vantaa, Finland Tel. +358-9-329 100, Fax +358-9-3291 0415 www.thermo.com

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

5

Chapter 1: Safety Symbols and Markings
1 SAFETY SYMBOLS AND MARKINGS
These symbols are intended to draw your attention to essential information and alert you to the presence of hazards as indicated. SAFETY SYMBOLS USED IN THE MULTIDROP 384
Power ON
Power OFF
WEEE symbol This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96/EC.
WARNING MARKINGS USED IN THE DOCUMENTATION Caution: risk of electric shock.
Caution: biohazard risk.
Caution: risk of personal injury to the operator or a safety hazard to the surrounding area. Caution: risk of damage to the instrument, other equipment or loss of performance or function in a specific application.

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

6

Chapter 2: About the User Manual
2 ABOUT THE USER MANUAL
This user manual has been written for the end user (for example, laboratory technician) and provides information on the Thermo Scientific Multidrop 384 microplate dispenser. This manual contains the installation and operating instructions for the Multidrop 384 microplate dispenser.
Read the manual in its entirety prior to operating the instrument.
This user manual has been designed to give you the information you need to:
· Review safety precautions · Install the Multidrop 384 · Carry out dispensing procedures · Recalibrate the dispensing cassette · Perform basic maintenance procedures · Troubleshoot the instrument performance
This user manual also describes features and specifications of the Multidrop 384 hardware and onboard software.
Chapter 6 ROUTINE OPERATION explains the dispensing principles and procedures.
In Chapter 9 TROUBLESHOOTING you will find a problem-solving guide. The user should be familiar with the contents of Chapter 7 on maintenance.
For warranty and ordering information refer to Chapters 10 WARRANTY CERTIFICATE and 11 ORDERING INFORMATION.
In an effort to produce useful and appropriate documentation, we appreciate your comments on this document to your local Thermo Fisher Scientific representative.

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

7

Chapter 3: Introduction to the Multidrop 384
3 INTRODUCTION TO THE MULTIDROP 384
There are two models of the Multidrop 384: · 5840150 Multidrop 384, 220240 V 50/60 Hz · 5840157 Multidrop 384, 110120 V 50/60 Hz
Each Multidrop 384 comes with one dispensing cassette: · 24072670 Standard tube dispensing cassette (with 40 cm tubing set)
3.1 Intended use
The Multidrop 384 microplate dispenser is intended for professional research use by trained personnel. The instrument is intended for automated dispensing of up to eight different reagents simultaneously into microplates and strips in 96 or 384-well plate format. Use for self-testing is excluded.
3.2 Principle of operation
The Multidrop 384 is an automatic, programmable, eight-channel microplate dispenser. It has a peristaltic pump for rapid and continuous dispensing of liquids into 96 and 384-well microplates. It can dispense 1 to 8 different reagents from external liquid reservoirs into different rows and can be used in most microtitration techniques, for example, in the fields of bacteriology, cell biology, hematology, immunology, toxicology and virology. It is particularly useful in the pharmaceutical industry. The Multidrop 384 is lightweight, transportable and compact on a laboratory bench. It can dispense 20 µl into the entire 384-well microplate in 20 seconds or 20 µl into the entire 96-well microplate in 5 seconds.

Fig. 3.1 Multidrop 384 microplate dispenser

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

8

Chapter 3: Introduction to the Multidrop 384
3.3 Advantages of using the Multidrop 384
The Multidrop 384 microplate dispenser provides several advantages relating mainly to the principle of operation in that it has:
· Superior accuracy and precision · A flexible volume range · High-speed continuous dispensing · Robot compatibility and versatile remote control commands · An autoclavable, detachable and calibrateable dispensing cassette
The Multidrop 384 is an automated microplate dispenser designed for high- throughput screening and microvolume dispensing into 384 and 96-well plates.
The Multidrop 384 can dispense up to eight different reagents with high accuracy, precision and speed. It fills a 384-well plate with 20 µl/well in 20 seconds or a 96-well plate in 5 seconds. With a volume range of 5 100 µl for 384-well plates and 5 395 µl for 96-well plates, the Multidrop 384 offers superior flexibility for a wide range of applications.
All reagent lines can be backflushed to the reagent bottle, minimizing the loss of expensive reagents. From 1 to 24 columns can be selected for 384-well plates and from 1 to 12 columns for 96-well plates.
To ensure sterility and avoid cross contamination, the dispensing cassette is detachable and autoclavable.
The Multidrop 384 can be used as a standalone instrument or added to robotic systems via an RS-232 serial port.

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

9

4 FUNCTIONAL DESCRIPTION
4.1 Instrument layout 4.1.1 Front view

15

12

11

10

9 13 14

Chapter 4: Functional Description

4

1

3

2

16

5

8 7

6

1 Instrument cover 2 Control panel
3 Thumbwheels for selecting the volume (volume µl) and number of columns (col) 4 Plate switch (plate) for selecting the 96/384-well plate (= rocker switch)
5 Plate carrier 6 Plate adapter 7 Plate adapter lock 8 Transfer rails 9 Priming vessel 10 Lower part slots 11 Upper part slots 12 Rest position slots of the upper part 13 Pump rotor 14 Rotor needles 15 Rotor cover not pulled over the rotor 16 Pump body
Fig. 4.1 Multidrop 384 front view (A)

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

10

Chapter 4: Functional Description

2 1 2
3
1 Mains switch (ON/OFF) 2 Instrument cover retaining screws 3 Cooling-air outlet
Fig. 4.2 Multidrop 384 front view (B)

1 2 3
4

1 Rotor cover pulled over the rotor 2 Standard tube dispensing cassette attached 3 Plate adapter 4 Plate carrier
Fig. 4.3 Multidrop 384 front view (C)

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

11

Chapter 4: Functional Description

3

2

4

1

5

8 6

7

1 Tips (8) 2 Lower part of dispensing cassette with tips 3 Tension limiting wires (2) 4 Upper part of dispensing cassette with tubes 5 Sight strips (8) 6 Tubes (A H) 7 Tubing weight, eight tubes 8 Calibration screw cover
Fig. 4.4 Close-up of the Multidrop 384 standard tube dispensing cassette

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

12

4.1.2 Back view

Chapter 4: Functional Description

1

3

2

1 Mains input socket 2 Fuses in the fuse holder cap 3 RS-232C serial interface connector
Fig. 4.5 Multidrop 384 rear view

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

13

Chapter 4: Functional Description
4.2 Multidrop 384 microplate dispenser
The Multidrop 384 microplate dispenser (Fig. 4.6) automates dispensing into microplates. The microprocessor-based system permits volumes between 5 100 µl for 384-well plates and 5 395 µl for 96-well plates to be selected (in 5 µl increments) and then dispensed simultaneously into eight wells (one column of a 96-well microplate). Setting the volume to be dispensed and the number of columns per plate (maximum 12 columns in 96-well plates and 24 columns in 384-well plates) is carried out using thumbwheels.
The movement of the microplate is automatic:
In the 96-well plate mode, the columns with 8 wells are filled starting from column 1 up to the column number set, after which the plate carrier will return to the home position. In the 384-well plate mode, the columns with 16 wells must be filled in two phases: first every other row (A, C, …, M, O) is filled starting from column 1 up to the set column number. Then the plate carrier shifts sideways so that the remaining rows (B, D, …, N, P) are filled in the opposite order, from the set column number back to column 1.
Normally, the microplate moves through the instrument automatically, but a semiautomatic mode is
possible by using the step and drop keys, which control dispensing into any column of the plate.
The dispensing cassette contains eight individual tubes. The dispensing cassette is detachable. Each reagent can have a separate dispensing cassette. Alternatively, the same dispensing cassette can be used with several reagents using the backflush feature. Refer to section 11.2 Dispensing cassettes.
The latest technology has been used in the manufacture of the dispensing cassette which includes an injection molded manifold. All dispensing cassettes can be autoclaved and Thermo Fisher Scientific guarantees them up to 50 autoclavings. After this amount it is recommended you replace the cassette.
To avoid damaging the tubes, follow the dispensing cassette maintenance, washing, autoclaving and storage instructions carefully (see the instructions in sections 6.8.4 After use, 7.2 How to wash the dispensing cassette and 7.3 How to autoclave the dispensing cassette).

Fig. 4.6 Multidrop 384 microplate dispenser

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

14

Chapter 5: Installation

5 INSTALLATION

5.1 Installation check list

The chapter on installation will contain an outline of the points mentioned in the check list below.

Tick Item

T

Unpack the Multidrop 384 instrument carefully. Refer to 5.2.1. Keep the original packaging

and packing material for future transportation.

T

Check the delivery for completeness. Refer to 5.2.2.

T

Check for damage during transport. Refer to 5.2.3.

T

Place the dispenser on a normal laboratory bench taking into account both the environmental

and technical prerequisites. Refer to 5.2.4 and 5.2.6. Leave sufficient clearance on both sides

and at the rear of the unit.

T

Install the instrument. Refer to 5.3.1.

T

Connect the mains supply cable to the mains input socket. See 5.3.2.

T

Carry out the operational check. Refer to 5.4.

5.2 What to do upon delivery
5.2.1 How to unpack
Move the unpacked instrument to its site of operation. Unpack the Multidrop 384 instrument and accessories carefully with the arrows on the transport package pointing upwards. The following notes and instructions are sent with the instrument and are immediately available when you open the package:
· Warranty Certificate card · packing instructions/packing list · Multidrop 384 User Manual
Caution: Do not touch or loosen any screws or parts other than those specially designated in the instructions. Doing so might cause misalignment and will invalidate the instrument warranty.

Retain the original packaging and packing material for future transportation. The packaging is designed to assure safe transport and minimize transit damage. Use of alternative packaging materials may invalidate the warranty. Also retain all instrument-related documentation provided by the manufacturer for future use.

5.2.2 Checking delivery for completeness
Check the enclosed packing list against order. If any parts are missing, contact Thermo Fisher Scientific Oy or your local Thermo Fisher Scientific representative.

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

15

Chapter 5: Installation
5.2.3 Checking for damage during transport
Visually inspect the transport package, the instrument and the accessories for any possible transport damage.
If the carton has been damaged in transit, it is particularly important that you retain it for inspection by the carrier in case there has also been damage to the instrument.
Neither the manufacturer nor its agents can be held responsible for any damage incurred in transit, but the manufacturer will make every effort to help obtain restitution from the carrier. Upon receipt of the carrier’s inspection report, arrangements will be made for repair or replacement.
Visually check all interconnections in the basic instrument. Check that there are no loose parts inside the instrument.
If any parts are damaged, contact Thermo Fisher Scientific Oy or your local Thermo Fisher Scientific representative.
5.2.4 Environmental requirements
When you set up your Multidrop 384, avoid sites of operation with excess dust, vibrations, strong magnetic fields, direct sunlight, draft, excessive moisture or large temperature fluctuations.
· Make sure the working area is flat, dry, clean and vibration-proof and leave additional room for accessories, cables, reagent bottles, etc.
· Make sure the ambient air is clean and free of corrosive vapors, smoke and dust. · Make sure the ambient temperature range is between +10°C (50°F) and +40°C (104°F). · Make sure relative humidity is between 10% and 80% (non- condensing).
Leave sufficient space (at least 10 cm) on both sides and at the back of the unit to allow adequate air circulation.
The Multidrop 384 does not produce operating noise at a level that would be harmful. No sound level measurements are required after installation.
Warning: Do not operate the instrument in an environment where potentially damaging liquids or gases are present.
5.2.5 Things to avoid
Do not smoke, eat or drink while using the Multidrop 384. Wash your hands thoroughly after handling test fluids. Observe normal laboratory procedures for handling potentially dangerous samples. Use proper protective clothing. Use disposable gloves. Be sure the working area is well-ventilated.
Never spill fluids in or on the equipment.
5.2.6 Technical prerequisites
Place the instrument on a normal laboratory bench. The net weight of the entire equipment is approx. 6.2 kg [13.7 lbs.] and the dispensing cassette 124 g [0.3 lb.].
The instrument operates at voltages of 100 ­ 240 Vac and a frequency range of 50 ­ 60 Hz.

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

16

Chapter 5: Installation
5.3 Setups before you put the instrument into operation 5.3.1 Installation procedure
The following procedure may be carried out by any properly skilled person using standard tools. In case the local voltage does not correspond to the label at the rear of the instrument on top of the mains input socket (Fig. 4.5), follow the next installation procedures.
Warning: Ensure the mains switch (Fig. 4.2) on the rear of the left side panel is in the OFF position. Also ensure the mains supply cable is disconnected (Fig. 5.3). 1. Remove the instrument cover retaining screws (Fig. 5.1 and Fig. 4.2) and lift up the cover.
Cover retaining screw on both sides
Fig. 5.1 Location of the cover retaining screws 2. Check that the voltage selector switch (Fig. 5.2) is in the correct position, that is, the voltage
selected corresponds to the local voltage. Voltage

Fig. 5.2 Location of the voltage selector

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

17

Chapter 5: Installation
3. Visually check that you did not leave any loose parts inside the instrument. 4. Close the cover and fasten the cover retaining screws.
5.3.2 How to ensure startup
1. Warning: Ensure the mains switch (Fig. 4.2) on the rear of the left side panel is in the OFF position.
2. Connect the mains supply cable to the mains input socket (Fig. 4.3 and Fig. 5.3) at the bottom of the back panel. If you need to use any other type of mains supply cable than supplied, use only cables certified by the local authorities.
3. Connect the instrument to a correctly installed line power outlet that has a protective conductor that is grounded.
Warning: Never operate your instrument from a power outlet that has no ground connection. Never use a mains supply cable other than the Thermo Scientific mains supply cable designed for your region.

Fig. 5.3 Connecting the mains supply cable

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

18

Chapter 5: Installation

5.4 Operational check

The following procedure should be completed without the dispensing cassette to confirm the correct functioning of the dispenser prior to normal use.

1

Connect the mains supply cable (Fig. 5.3). Switch the instrument on using the mains switch (Fig. 4.2).

· If the instrument starts:
– The green power LED will light up
– The plate carrier will return to the home position

· If the instrument does not start:
)Pull the rotor cover over the rotor )Check the mains cable connection

2 Press prime/drop to prime
– The pump rotor will rotate
· If the pump rotor does not rotate:
)Pull the rotor cover over the rotor
· If the red error LED lights up: )Insert the priming vessel, press the stop key and prime again

3 Press start
– Starts the dispensing cycle
· If the dispensing cycle does not start:
)Pull the rotor cover over the rotor
· If the red error LED lights up: )Press the stop key, then press the prime key and finally press the start key again )Insert the priming vessel, press the stop key, then press the prime key and finally press the
start key again

4 plate (plate switch)
– Dispenses liquid into the 384 or 96-well microplates
– Try the alternative plate formats by pressing the start key

5 step
– Advances the microplate by one column · If the microplate does not advance:
)Pull the rotor cover over the rotor
6 After step press prime/drop
– To dispense a preselected volume into the column selected by steps – The plate carrier will return to the home position after reaching the last column
After step press start (partial filling of the tubing)
– To dispense the selected volume automatically from the set column to the last column

7 empty
– The pump rotor will rotate
· If the pump rotor does not rotate:
)Pull the rotor cover over the rotor

8 stop
– Terminates the operation and returns the plate to the home position For more information on the control panel, refer to section 6.5 How to use the control panel.

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

19

Chapter 5: Installation
5.5 Operating precautions and limitations before operation
1. Read this manual in its entirety, as it contains information necessary to ensure safe operation. 2. Always ensure that the local supply voltage in the laboratory conforms to that specified on the type
label on the back of the instrument.

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

20

Chapter 6: Routine Operation

6 ROUTINE OPERATION

6.1 Operational check list

The chapter on routine operation will contain an outline of the points mentioned in the check list below.

Tick Item

T

Switch on the instrument. Refer to 6.2.

T

Insert the priming vessel and the dispensing cassette. Refer to 6.3.

T

Use the plate adapter for the plate carrier to function. Refer to 6.4.

T

Pull the rotor cover over the rotor. Refer to 6.3.

T

Familiarize yourself with the control panel. Refer to 6.5.

T

Press the plate switch (plate) to position 96 or 384 according to the plate type you use.

Refer to 6.5.2.

T

Prime all the eight channels so that there are no air bubbles in the tubes. Refer to 6.7.

T

Carry out dispensing either manually or automatically. Refer to 6.8.

T

After all the plates have been dispensed, press the empty key to return the reagent or liquid

from the tubes to the reservoir. Refer to 6.8.4.

T

Wash the dispensing cassette by priming it with deionized distilled water when not in use.

Refer to 6.8.4 and 7.2.

T

After washing, store the dispensing cassette in the rest position. Refer to 6.8.4 and 7.2.

T

Maintain the instrument regularly. Refer to Chapter 7.

Note: Keep the dispensing cassette in the rest position when the dispensing cassette is not in use.

6.2 Switching on
Switch on the instrument by using the mains switch on the left-hand side of the instrument (Fig. 4.2).
The green power LED will light up.

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

21

Chapter 6: Routine Operation
6.3 How to insert the priming vessel and the dispensing cassette
1. Ensure that the priming vessel is inserted correctly into its slot on the left of the plate carrier (Fig. 6.1). You have fastened it correctly when it snaps slightly and is inserted into the sensor slot.
Priming vessel sensor slot
Fig. 6.1 Inserting the priming vessel into place 2. Take the lower part of the cassette into your right hand with the dispensing tips pointing down
and the upper part into your left hand (Fig. 6.2).

Fig. 6.2 Inserting the standard tube dispensing cassette

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

22

Chapter 6: Routine Operation 3. Carefully place the eight tubes below the pump rotor and insert the lower part of the cassette into
the lower part slots of the pump body (Fig. 6.3). Check that the tubes are freely placed below the rotor and the tension limiting wires below the rotor shaft.
Lower part slots
Fig. 6.3 Inserting the lower part of the dispensing cassette into its slots 4. Take a firm grip on the upper part of the cassette with your right hand and carefully pull the tubes
around the rotor until the upper part reaches and fits into the upper part slots of the pump body (Fig. 6.4 and Fig. 4.1).

Fig. 6.4 Inserting the upper part of the dispensing cassette

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

23

Chapter 6: Routine Operation
Note: The tension limiting wires are designed to prevent the user from pulling the tubes too much as excessive tension may damage the tubes or change the calibration of the cassette. 5. Double-check that both the upper and the lower parts are properly placed into their corresponding slots (Fig. 6.3 and Fig. 6.5).
Upper part slots
Fig. 6.5 Lower and upper parts of the dispensing cassette inserted evenly into their slots 6. Ensure that all the tubes are evenly placed on the rotor needles, four tubes on each half of the
rotor. 7. Ensure also that the tension limiting wires have a loose fit around the rotor shaft. 8. PULL THE ROTOR COVER OVER THE ROTOR (Fig. 6.6). The plate carrier will then return to
the furthest right of the instrument, that is, the home position. The microswitch controls that the cover is properly placed over the rotor. The Multidrop 384 is now ready for priming.

Fig. 6.6 Pulling the rotor cover over the rotor

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

24

Chapter 6: Routine Operation
Note: If the rotor cover is not pulled over the rotor, the instrument will not function.
Note: It is recommended you change the dispensing cassette or tubing set after dispensing about 3000 ­ 5000 96-well plates, depending on the dispensing frequency and the liquids used. Note: Keep the dispensing cassette in the rest position when the dispensing cassette is not in use.
6.4 How to insert and adjust the plate adapter
The Multidrop 384 requires a plate adapter for the plate carrier to function. Two different plate adapters are included (Fig. 6.7).
The PLATE ADAPTER, PLATE HEIGHT MAX. 15 MM, is suitable for plates approximately 15 mm high, for example, 96well plates and some 384-well plates.

Fig. 6.7 Two different plate adapters

The PLATE ADAPTER, PLATE HEIGHT MAX. 11 MM, is suitable for lower plates, for example, 384-well plates.

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

25

Chapter 6: Routine Operation
The plate adapter is inserted into the plate carrier in the following way: 1. Open the plate adapter lock located in the front left corner of the plate carrier (Fig. 6.8). Select
the plate adapter according to the height of the plate.

Fig. 6.8 Opening the plate adapter lock
2. Take the plate adapter in your left hand and lower the right end. Place the plate adapter into the right end corner of the plate carrier (Fig. 6.9).

Plate adapter lock Fig. 6.9 Placing the right end of the plate adapter

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

26

Chapter 6: Routine Operation 3. Then press the left end of the plate adapter onto the plate carrier (Fig. 6.10).
Fig. 6.10 Pressing the left end of the plate adapter 4. Lock the plate adapter into place with the plate adapter lock located in the front left corner of the
plate carrier (Fig. 6.11).

Fig. 6.11 Closing the plate adapter lock

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

27

Chapter 6: Routine Operation 5. To adjust the plate size, loosen first the two attached plastic-covered screws on the right of the
plate carrier (Fig. 6.12).
Fig. 6.12 Loosening the screws on the right of the plate carrier 6. Then loosen the two attached plastic-covered screws at the front of the plate adapter (Fig. 6.13).

Fig. 6.13 Loosening the screws at the front of the plate adapter

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

28

Chapter 6: Routine Operation
7. Insert the microplate onto the plate adapter. Push the microplate at the same time towards the left-hand top corner of the plate adapter while you fasten the front screws (Fig. 6.14).

Fig. 6.14 Fastening the front screws of the plate adapter
8. Then fasten the screws to the right of the plate carrier, pushing the microplate simultaneously towards the left-hand corner of the plate adapter (Fig. 6.15). If the instrument is used for robotics, leave sufficient moving space for the microplate according to the robotic specifications.

Fig. 6.15 Fastening the screws to the right of the plate carrier
Note: Ensure the dispensing cassette is attached and the rotor cover has been pulled over the rotor.

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

29

Chapter 6: Routine Operation
6.5 How to use the control panel
The keypad has an embossed, tactile membrane with five keys. There are two control panel indicators (LEDs) in all. The control panel is shown in Fig. 6.16.

Fig. 6.16 Multidrop 384 control panel

6.5.1 Control panel indicators

power

The green power LED indicates that the dispenser is powered.

error

The red error LED shows when the dispenser has some kind of error.

6.5.2 Control panel thumbwheels (digital switches)

plate
volume µl col

96/384-well switch: sets the plate carrier movement and dispensing for 96 or 384well plates.
Reagent volume thumbwheels: sets the volume to be dispensed per well.
Column thumbwheels: sets the number of columns per plate, up to 12 with 96well plates or up to 24 with 384-well plates.

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

30

Chapter 6: Routine Operation

6.5.3 Control panel keys

start prime/ drop
empty step stop

Starts the dispensing cycle.
Primes the cassette if the tips are above the priming vessel. Primes continuously as long as the user presses the key. Dispenses the adjusted volume if the tips are above the wells.
Empties the liquid from the tubes to the liquid reservoir.
Advances the microplate by one column.
Terminates the operation and returns the plate to the home position.

6.6 How to select the 96 / 384-well plate
· Press the plate switch (plate) down into position 96 or 384 according to the plate type to be used
(Fig. 6.17).
· Select the number of columns (col) between 1 and 24 in the 384 mode.
· Select the number of columns (col) between 1 and 12 in the 96 mode (Fig. 6.17). Note that if you set the column selection above 12 in the 96 mode, the red error light will light up and the
dispensing operation will be terminated.

Fig. 6.17 Multidrop 384 control panel

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

31

Chapter 6: Routine Operation

6.7 How to prime
Note: Ensure that the instrument is sufficiently level on the table to prevent liquids from overspilling when priming.
Note: Ensure that the liquid temperature is close to the calibration temperature of 22°C. Differences greater than ± 2°C will cause an error in the results.
Note: Silicon tubing (silicon rubber) is resistant to a number of chemical liquids. Most water solutions typically used in a medical laboratory can be dispensed with the Multidrop 384. Some strong substances may, however, harm the silicone tubing and cause swelling or breaking. If the substance has even a slight effect on silicone, the calibration of the cassette may be affected and the cassette may become unusable.
1. Place the eight tube ends into the same liquid reservoir or into different reservoirs taking care that all tube ends are below the liquid level. Use the tubing weight provided if possible.
2. Check that the priming vessel is firmly inserted next to the plate carrier. The Multidrop 384 will not operate if the priming vessel is not completely inserted into its slot.
3. Press the prime/drop key until all eight channels are dispensing continuously into the priming
vessel and there are no more air bubbles in the tubes. It is recommended you fill the priming vessel two or three times with the reagent to be used before starting the actual dispensing in order to bring the tubes into working condition.
Note: If you press the prime/drop key at the same time as the rotor cover is pulled over
the rotor, the program enters into the volumetric calibration mode.
Note: You can empty the contents of the priming vessel back into the reagent reservoir by pulling the priming vessel out of its slot if necessary. It is recommended you do this if you use expensive reagents. Therefore, wash the priming vessel always after each use to avoid any cross contamination. If you use several liquids at the same time in different rows, you can order a special eight-cavity priming vessel for this purpose to prevent mixing the liquids. Also a self-draining (flow-through) priming vessel is available. Refer to section 11.4 Priming vessels (consumables).
6.8 Dispensing
6.8.1 How to dispense automatically
1. Set the required reagent volume per well. The permitted volume range is 5 100 µl for 384-well plates and 5 395 µl for 96-well plates.
2. Set the required number of columns to be dispensed per plate.
3. Place the microplate onto the plate carrier equipped with a suitable plate adapter. Refer to section 6.4 How to insert and adjust the plate adapter.
4. Press the prime/drop key to prime the tubes. Refer to section 6.7 How to prime.

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

32

Chapter 6: Routine Operation
5. Press the start key. The Multidrop 384 will now automatically dispense the preselected volume
of reagent into the preselected number of columns. When dispensing has been completed, the plate carrier will automatically return to its home position.
6. Now you can dispense any number of plates by only changing the plate and pressing start.
Note: Check that there is always enough liquid in the reservoir and ensure that all the tube ends are below the liquid level. Use the tubing weight supplied.
Note: Check after each dispensing that the priming vessel has not filled up. Empty it if necessary, otherwise the liquids may spill over during operation. Note that the Multidrop 384 always primes itself before each plate filling to ensure maximum accuracy and precision. It is recommended you empty the priming vessel after dispensing 30 ­ 40 plates. Alternatively, use the self-draining priming vessel.
6.8.2 How to dispense manually into the specified column(s)
1. Set the desired reagent volume. The permitted volume range is 5 100 µl in the 384-well plate mode and 5 395 µl in the 96-well plate mode.
2. Place the microplate onto the plate carrier equipped with a suitable plate adapter.
3. Use the step key to move the plate carrier column by column until you reach the desired
column.
4. Press the prime/drop key. The Multidrop 384 will now dispense the preselected volume into
the column desired. The plate carrier will return to the home position after reaching the last
column. If you use the start key, the instrument will automatically dispense the selected volume
from the set position to the last column selected.
Note: If you have pressed either the stop or empty key, the Multidrop 384 refuses to start dispensing before you have pressed the prime/drop key once again.
6.8.3 How to dispense multiple volumes
Ten- or hundredfold volumes can be dispensed compared to the volume chosen.
6.8.3.1 Dispensing tenfold and hundredfold volumes
1. Press the stop and prime/drop keys simultaneously.
2. Having done that first, release the stop key and then the prime/drop key. Note that the
Multidrop 384 is in the volumetric calibration mode.
3. Now you can push the plate carrier into the desired dispensing position.
4. Set one tenth of the volume required on the thumbwheel. Then press the start key and a tenfold
volume will be dispensed.
5. Set one hundredth of the volume required on the thumbwheel. Then press the step key and a
hundredfold volume will be dispensed.

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

33

Chapter 6: Routine Operation
6.8.4 After use
After all the plates have been dispensed, press the empty key to return the reagent or liquid from the
tubes to the reservoir. This saves expensive reagents.
If there is only a short break in dispensing, no washing or cleaning of the tubes is necessary.
However, after pressing the empty key, release the tubing tension by pulling the upper part of the
cassette from its slots and place it into its rest position slots located on the left-hand side of the rotor (Fig. 6.18).
If there is a longer break (for example, a day), wash the dispensing cassette by priming it with deionized distilled water or with special washing detergent if necessary. In the end, rinse the washing detergent away with deionized distilled water.
After washing, store the dispensing cassette in the rest position (Fig. 6.18).
Note: Keep the dispensing cassette in the rest position when the dispensing cassette is not in use.

Fig. 6.18 Rest position of the dispensing cassette

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

34

Chapter 6: Routine Operation
6.9 Shutdown
Remove any microplates still on the plate carrier. Dispose of all microplates and strips as biohazardous waste.
After all the plates have been dispensed, press the empty key to return the reagent or liquid from the
tubes to the reservoir. This saves expensive reagents. If there is only a short break in dispensing, no washing or cleaning of the tubes is necessary.
However, after pressing the empty key, release the tubing tension by pulling the upper part of the
cassette from its slots and place it into its rest position slots located on the left-hand side of the rotor (Fig. 6.18). If there is a longer break (for example, a day), wash the dispensing cassette by priming it with deionized distilled water or with special washing detergent if necessary. After washing, store the dispensing cassette in the rest position (Fig. 6.18). Switch the Multidrop 384 off by pressing the mains switch (Fig. 4.2) at the end of the left side panel of the instrument into the OFF position. Wipe the instrument surfaces with a soft cloth or tissue paper moistened with deionized distilled water, a mild detergent (SDS, sodium dodecyl sulfate) or soap solution. If you have spilt infectious agents on the dispenser, disinfect with 70% alcohol or some other disinfectant (see section 7.8 Decontamination procedure).
6.10 Guidelines for use
· When the error LED is on, the start or step keys will not function.
· To clear the error and continue the dispensing procedure, press the stop key and wait until the
plate carrier has returned to the home position. The dispensing procedure can then be restarted.
· If you press the stop key, all operations will stop. The plate carrier is automatically returned to
the home position. The Multidrop 384 will now require priming again.
· Preventative maintenance should be followed closely to keep the microplate dispenser in the best condition for maximum reliability. A poorly maintained instrument will not give the best results.

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

35

Chapter 7: Maintenance
7 MAINTENANCE
7.1 Regular and preventive maintenance
7.1.1 General
Routine and service procedures must be performed by the user to prevent unnecessary wear or hazards and are described below at the frequency with which they should be applied. Abrasive cleaning agents are not recommended, because they are likely to damage the paint finish. It is recommended you clean the case of the instrument periodically to maintain its good appearance. A soft cloth dampened in a warm, mild detergent solution will be sufficient. Clean the outside of the instrument and the plate carrier with clean low- pressure compressed air or a cloth dampened with water or a mild detergent when necessary.
Painted surfaces can be cleaned with most laboratory detergents. Dilute the cleaning agent as recommended by the manufacturer. Do not expose painted surfaces to concentrated acids or alcohols for prolonged periods of time as damage may occur.
Clean the control panel with a mild laboratory detergent. Plastic covers and surfaces can be cleaned with a mild laboratory detergent or alcohol.
If any surfaces have been contaminated with biohazardous material, a mild sterilizing solution should be used.
The dispensing cassette is autoclavable. Do not autoclave any other part of this instrument.
7.1.2 Immediate
Although the Multidrop 384 is constructed from high-quality materials, you must immediately wipe away spilled saline solutions, solvents, acids or alkaline solutions from outer surfaces to prevent damage and wipe with deionized distilled water.

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

36

Chapter 7: Maintenance
7.2 How to wash the dispensing cassette
Wash the tubes by priming them first with tap water and then with deionized distilled water. Make sure that all the tubes are washed properly. If cleaning with water is insufficient, use a mild laboratory detergent and then prime with large amounts of tap water followed by deionized distilled water. The dispensing cassette can be dried at room temperature. After washing, store the dispensing cassette in the rest position (Fig. 6.18). If the instrument is not used for a couple of hours, insert the dispensing cassette into the rest position. Refer to section 6.8.4 After use. Handle the dispensing cassette with great care so that you do not damage the tubes and dispensing tips. These factors will affect the service life of the dispensing cassette.
7.3 How to autoclave the dispensing cassette
The standard tube dispensing cassette (Cat. no. 24072670) can be autoclaved 50 times with the same tubes. The autoclaving conditions of the dispensing cassette are as follows: 1 bar pressure at 121°C for 20 minutes.
Note: After autoclaving, the dispensing cassette must cool down to room temperature before use.

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

37

Chapter 7: Maintenance
7.4 How to replace the fuses
1. Switch off (Fig. 4.2) and unplug the instrument from the mains input socket (Fig. 4.5). 2. Remove the fuse holder cap with a screwdriver or similar tool. Press both ends of the fuse holder
cap with the tool (Fig. 7.1 and Fig. 7.2).
Fig. 7.1 Removing the fuse holder cap (A)

Fig. 7.2 Removing the fuse holder cap (B)

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

38

3. Check the fuses in the fuse holder cap (Fig. 7.3).

Chapter 7: Maintenance

Fig. 7.3 Fuse holder cap with fuses
4. Replace the blown fuse with the same type and push the fuse holder cap back into its position (Fig. 7.4).

Fig. 7.4 Inserting the fuse holder cap 5. Reconnect to the mains input socket and switch the power on.

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

39

Chapter 7: Maintenance
7.5 How to recalibrate the dispensing cassette
The dispensing cassette has been calibrated by the manufacturer with deionized distilled water at 22°C ± 2°C. It is recommended you recalibrate the cassette after dispensing about 1000 96-well plates or after every three months. It is also recommended you recalibrate the dispensing cassette if you have forgotten the cassette in the Multidrop 384 under tension for prolonged periods, for example, a weekend or longer.
Two different calibration methods are described below, that is, volumetric calibration and gravimetric calibration.
7.5.1 Volumetric calibration
For volumetric calibration, use the following steps:
1. Remove the priming vessel and microplate.
2. Install the dispensing cassette into the Multidrop 384 if it is not already installed. Place the dispensing cassette into the rest position (Fig. 6.18). Refer to section 6.3 How to insert the priming vessel and the dispensing cassette.
3. Install the tube of the calibration device into the tip of the channel to be recalibrated (Fig. 7.5), for example, A.

Fig. 7.5 Placing the tube onto the tip of channel A
4. Insert the dispensing cassette into the working position so that the lower and upper parts of the dispensing cassette are inserted into their slots (Fig. 6.5).
5. Pull the rotor cover over the rotor.
6. Place the tube of channel A into the reservoir with water at room temperature (working temperature).

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

40

Chapter 7: Maintenance
7. Open the calibration screw cover on the upper part of the cassette (Fig. 7.7 and Fig. 4.4). 8. Set the volume to 100 µl.
9. Press the stop and prime/drop keys at the same time. After that first release the stop key and then the prime/drop key. This brings the Multidrop 384 into the volumetric calibration
mode.
10. Press the start key as many times until the water enters the calibration pipette. Note: Take care not to press the step key accidentally or otherwise 10 ml of water will be
dispensed.
11. Adjust the water level in the pipette to the lower mark by pulling or pushing the syringe piston (Fig. 7.6).
Upper mark

Lower mark

Fig. 7.6 Adjusting the liquid level first to the lower mark and then to the upper mark
12. Press the start key and wait until the water level stops. It should be at the upper mark, 1000 µl
(± 1.5%) (Fig. 7.6).
13. If the volume is higher than 1000 µl, turn the calibration screw (Fig. 7.7) clockwise with a screwdriver; one turn for each 10 µl (1% from the volume dispensed); if the volume is lower than 1000 µl, turn in the opposite direction.

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

41

Calibration screw cover opened

Chapter 7: Maintenance

Fig. 7.7 Screwing the calibration screws according to the calibration results
14. Press the empty key and adjust the water level in the pipette to the lower mark and repeat Steps
10 to 13 until the result is 1 ml. Three accurate calibrations must be obtained. 15. Change the tube to the next channel and repeat Steps 10 to 14.
16. After all the channels have been recalibrated, press the stop key to reset the Multidrop 384 into
normal operation and reinstall the priming vessel. 17. Close the calibration screw cover.
7.5.2 Gravimetric calibration
For gravimetric calibration, use the following steps: 1. Install the dispensing cassette into the working position so that the lower and upper parts of the
dispensing cassette are inserted into their slots (Fig. 6.5). 2. Pull the rotor cover over the rotor. 3. Ensure that the priming vessel is installed. Refer to section 6.3 How to insert the priming vessel
and the dispensing cassette. 4. Ensure that you have the plate adapter for 15 mm high plates installed. Refer to section 6.4 How
to insert and adjust the plate adapter.

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

42

Chapter 7: Maintenance
Note: Keep the dispensing cassette in the rest position when the dispensing cassette is not in use.
5. Place the tube of channel A into the reservoir with water at room temperature (working temperature).
6. Use 8 x 12 well microstrips and adjust the Multidrop 384 for 100 µl in 10 columns and set the
plate switch (plate) for 96-well plates (Fig. 7.8).

Fig. 7.8 8 x 12 well microstrips adjusted onto the plate adapter
7. Press the prime/drop key to prime the tubes. Refer to section 6.7 How to prime.
8. Open the calibration screw cover on the upper part of the cassette. 9. Tare an analytical balance (resolution 0.01 mg) with one empty, dry 1 x 12 well microstrip. 10. Place the microstrip into the microplate frame on the row corresponding to the channel to be
recalibrated (from A to H). Press the start key.
11. Weigh the filled microstrip (Fig. 7.9). The balance should now read 1000 mg ± 20 mg.

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

43

Chapter 7: Maintenance

Fig. 7.9 Removing the filled microstrip to be weighed
12. If the balance reads less than 980 mg, turn the calibration screw counterclockwise: one turn for each 10 mg (1% from the volume dispensed). If the balance reads more than 1020 mg, turn in the opposite direction (Fig. 7.10). Recheck the delivery after each adjustment using Steps 9 to 11. Three acceptable calibrations must be obtained.

Fig. 7.10 Screwing the calibration screw according to the calibration results 13. Continue in the same way with each channel until all eight channels have been recalibrated. 14. Close the calibration screw cover.

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

44

Chapter 7: Maintenance
7.6 How to change the tubing set 7.6.1 Changing the tip band
1. Place the dispensing cassette onto the laboratory bench with the dispensing tips pointing down. 2. Unfasten the four cover retaining screws of the lower part of the dispensing cassette (Fig. 7.11).

Fig. 7.11 Removing the cover plate of the lower part of the dispensing cassette
3. Remove the tip manifold, the tube fasteners and the rest of the tubing from the lower part of the dispensing cassette. Then detach the tip band from the tubing by pulling the tubes away from the tip band (Fig. 7.12).

Fig. 7.12 Removing the old tip band

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

45

Chapter 7: Maintenance 4. Replace the tip band (Fig. 7.13). The tips of the tip band must not be clogged in any way but be
clean to function correctly (Fig. 7.14).
Fig. 7.13 Tip band removed Insert tube here
Tip
Fig. 7.14 New tip band
7.6.2 Fitting the new tubing set step by step
1. First place the new tubing set onto the laboratory bench with the tip band attached (Fig. 7.15). Ensure that you have saved the eight calibration screws and the tubing weight from the previous tubing set.

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

46

Chapter 7: Maintenance
Tubing identification plate with tube lot number
Fig. 7.15 New tubing set with the tip band attached 2. Remove the four cover retaining screws on the lower part of the dispensing cassette, the tubes
with the tube fasteners and the tip manifold with the tips, the three cover retaining screws on the upper part of the dispensing cassette, the two tension limiting wires, the calibration screws and the tubes with the tube fasteners. Save the calibration screws and the tubing weight for further use when installing the new tubing set. 3. Insert the tip manifold so that the tips go through the holes in the lower part of the dispensing cassette (Fig. 7.16) and guide the tubes through the eight openings in the side wall by pressing the small cubic tube fasteners into place (Fig. 7.17). Ensure that each tube is in its designated opening (Fig. 7.18).
Tip band
Tube fasteners

Fig. 7.16 Inserting the tip manifold into the lower part of the dispensing cassette

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

47

Chapter 7: Maintenance

Fig. 7.17 Pressing the tube fasteners into place

Fig. 7.18 New tubing inserted into the lower part of the dispensing cassette 4. Attach the tension limiting wires into their slots (Fig. 7.19).
Slots for tension limiting wires

Fig. 7.19 Tension limiting wires attached

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

48

Chapter 7: Maintenance 5. Fasten the four cover retaining screws of the lower part of the dispensing cassette (Fig. 7.20).

Fig. 7.20 Replacing the lower part cover of the dispensing cassette
6. Once the lower part of the dispensing cassette has been fitted, place the remaining parts belonging to the upper part of the dispensing cassette onto the laboratory bench (Fig. 7.21).

Fig. 7.21 Parts belonging to the upper part of the dispensing cassette

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

49

Chapter 7: Maintenance 7. First place the plastic sight window with the grooves pointing upwards onto the bottom cover of
the upper part of the dispensing cassette, if it is not already there (Fig. 7.22).
Fig. 7.22 Placing the sight window 8. After that press the white tubing identification plate into place on the right-hand side of the bottom
cover of the upper part of the dispensing cassette (Fig. 7.23).

Fig. 7.23 Pressing the tubing identification plate into place

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

50

Chapter 7: Maintenance 9. Then press each tube fastener into place in its groove on the plastic sight window (Fig. 7.24).

Fig. 7.24 Pressing the tube fasteners into place
10. Fasten the calibration screws from the previous tubing into their places using a hexagonal screwdriver (Fig. 7.25).

Fig. 7.25 Fastening the calibration screws

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

51

Chapter 7: Maintenance 11. Insert the tension limiting wires into their slots into the upper part of the dispensing cassette
(Fig. 7.26). Slots for tension limiting wires
Fig. 7.26 Calibration screws fastened 12. Replace the upper part cover of the dispensing cassette carefully (Fig. 7.27).
Fig. 7.27 Replacing the cover of the upper part of the dispensing cassette 13. Ensure you do not get the tubing trapped or bent between the bottom and upper cover of the
upper part of the dispensing cassette (Fig. 7.28).

Fig. 7.28 Ensuring the tubing does not get trapped

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

52

Chapter 7: Maintenance 14. Fasten the three cover retaining screws of the upper part of the dispensing cassette, leaving the
front screw slightly open (Fig. 7.29).
Fig. 7.29 Fastening the cover retaining screws 15. Insert the calibration screw cover easier by lifting or bending the upper cover slightly (Fig. 7.30).

Fig. 7.30 Inserting the plate on the calibration screws

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

53

Chapter 7: Maintenance 16. Fasten the front cover retaining screw (Fig. 7.31). Continued below.
Fig. 7.31 Lower and upper part of the dispensing cassette fitted

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

54

Chapter 7: Maintenance 17. Turn the dispensing cassette so that the eight sight strips are visible (Fig. 7.32). Look at the eight
sight strips in the upper part of the dispensing cassette. You will notice that the tubes are not precalibrated. First open the calibration screw cover. For precalibration of the dispensing cassette, adjust the tube fasteners in the sight strips roughly to the middle using a hexagonal screwdriver (Fig. 7.33).
Sight
Fig. 7.32 Dispensing cassette before precalibration

Fig. 7.33 Precalibrating the dispensing cassette

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

55

Chapter 7: Maintenance
18. When the tube fasteners have all been adjusted roughly to the middle of the sight strip, close the calibration screw cover (Fig. 7.34).

Fig. 7.34 Dispensing cassette precalibrated
19. Insert the eight tubes into the tubing weight either at this stage or earlier (Fig. 7.35). The new tubing set has now been successfully fitted.

Fig. 7.35 Inserting the tubes into the tubing weight
20. Calibrate the dispensing cassette according to the instructions in section 7.5 How to recalibrate the dispensing cassette.

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

56

Chapter 7: Maintenance

7.7 Disposal of materials
Refer to local regulations for the disposal of infectious material.
The samples can be potentially infectious. Dispose of all used microplates, microstrips, disposable gloves, syringes, disposable tips, etc. as biohazardous waste.

7.8 Decontamination procedure

If there is any risk of contamination with biohazardous material, the procedure recommended below or some other corresponding efficient decontamination procedure must be performed.

You must perform the complete decontamination procedure before shipping the instrument to Thermo Fisher Scientific Oy, for example, for repair, or relocating the instrument from one laboratory to another. Refer to section 7.9 How to pack for service. Decontamination is not required for the proper functioning of the instrument.

Examples of decontaminants

· Ethanol · Virkon solution · Glutaraldehyde solution · Chloramine T · Microcide SQ

70% 1 3% 4%
1:64

IF LOCAL OR LABORATORY REGULATIONS PRESCRIBE REGULAR DISINFECTION, IT IS NOT ADVISABLE TO USE FORMALDEHYDE, SINCE EVEN SMALL TRACES OF FORMALDEHYDE AFFECT THE ENZYME BEING USED IN EIA TESTS IN A NEGATIVE WAY RESULTING IN BAD TEST RESULTS.

Always use disposable gloves and protective clothing and operate in a well- ventilated area.

Do not autoclave any other parts of the instrument except the dispensing cassette.

1. Prepare the decontaminant: for example, 1 3% Virkon solution or 200 ml 4% glutaraldehyde solution (or another agent recommended by your safety officer).
2. Empty the reagent container.
3. Switch the power OFF and disconnect the mains supply cable (Fig. 4.2).
4. Disinfect the outside of the instrument using a wad of cotton wool/cloth soaked in 70% ethanol.
5. Place the plate adapter and dispensing cassette, for example, in 1 3% Virkon solution or 200 ml 4% glutaraldehyde solution for 10 min and rinse them with deionized distilled water.
6. Place the entire instrument in a large plastic bag. Ensure that the reagent containers are disconnected and open.
7. Place a wad of cotton wool soaked in the prepared solution into the bag. Ensure that the wad does not make contact with the instrument.

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

57

Chapter 7: Maintenance
8. Close the bag firmly and leave the instrument in the bag for at least 24 hours.
9. Remove the instrument from the bag.
10. Clean the instrument using a mild detergent.
11. Remove any stains using 70% ethanol.
12. Flush the fluid path of the instrument with deionized distilled water using the priming procedure.
13. The dispensing cassette must be autoclaved.
14. Also wash and disinfect the reagent containers.
15. After performing this decontamination procedure, enclose a signed and dated Certificate of Decontamination both inside the transport package and attached to the outside of the package.
7.9 How to pack for service
When you ship the instrument for service remember to:
· Inform about the use of hazardous materials. · Decontaminate the instrument beforehand. · Pack the instrument according to the enclosed packing instructions. · Use the original packaging to ensure that no damage will occur to the instrument during shipping.
Any damage will incur additional labor charges. · Enclose a dated and signed Certificate of Decontamination (see section 9.4.1.1) both inside and
attached to the outside of the package, in which you return your instrument (or other items). · Enclose the return authorization number (RGA) given by the Thermo Fisher Scientific
representative. · Indicate the fault after you have been in touch with your local Thermo Fisher Scientific
representative or the Thermo Fisher Scientific technical service department.
See section 8.1 for details on storage and transportation temperatures.
7.10 Service contracts
It is recommended you maintain and service the instrument regularly every twelve months on a contract basis by the manufacturer’s trained service engineers. This will ensure the product is properly maintained and gives trouble-free service. Contact the Thermo Fisher Scientific technical service department for more details.
7.11 Disposal of the instrument
1. Decontaminate the instrument prior to disposal. See sections 7.8, 9.4.1 and 9.4.1.1 on decontamination. Also wash and disinfect the dispensing cassettes prior to disposal.
2. Follow laboratory and country-specific procedures for biohazardous or radioactive waste disposal. Dispose of the instrument according to the legislation stipulated by the local authorities concerning take-back of electronic equipment and waste. The proposals for the procedures vary by country.

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

58

Chapter 7: Maintenance
WEEE symbol Thermo Fisher Scientific has contracted with one or more recycling/disposal companies in each EU Member State (European Country), and this product should be disposed of or recycled through them. Further information on Thermo Fisher Scientific’s compliance with these Directives, the recyclers in your country, and information on Thermo Scientific products which may assist the detection of substances subject to the RoHS Directive are available at www.thermo.com/WEEERoHS.
3. Regarding the original packaging and packing materials, please use the recycling operators known to you.
4. For more information, contact your Thermo Fisher Scientific representative.

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

59

Chapter 8: Technical Specifications

8 TECHNICAL SPECIFICATIONS

8.1 General specifications

Overall dimensions

310 mm (12.2 in.) (W) x 320 mm (12.6 in.) (D) x 155 mm (6.1 in.) (H)

Weight (total)

Instrument 6.2 kg [13.7 lbs.]

Dispensing cassette 124 g [0.3 lb.]

Mains power supply

100 120 Vac, 50/60 Hz, nominal (operating range 90 130 Vac) 200 240 Vac, 50/60 Hz, nominal (operating range 180 256 Vac)

Power consumption 50 VA max.

Heat dissipation Fuses

171 BTU max.
2 x 300 mA for 200 240 V, UL 198G Time Delay, 5 x 20 mm 2 x 600 mA for 100 120 V, UL 198G Time Delay, 5 x 20 mm

Operating conditions +10°C +40°C (indoor use only)

Transportation conditions Storage conditions

-40°C +70°C, packed in transport packaging -25°C +50°C, packed in transport packaging

Autoclaving conditions of the dispensing cassette

pressure: 1 bar temperature: 121°C time: 20 min autoclavings: 50 times

Control panel

Keys: start, prime/drop, empty, step, stop Selection switches: plate (= 96/384), volume µl, col (= column)

Interface

Serial RS-232C

384-well dispensing

96-well dispensing

Dispensing volume Dispensing speed

5 ­ 100 µl in 5 µl increments 20 s/20 µl into 384 wells

5 ­ 395 µl in 5 µl increments 5 s/20 µl into 96 wells

Dispensing accuracy Dispensing precision

± 3% or ± 1 µl, whichever is greater 5 µl: ± 3% or ± 1 µl, whichever is greater 20 µl: ± 2% (typical) 100 µl: ± 1% (typical)
Valid when counted for the entire plate
5 µl: CV 10% or SD 0.6 µl, whichever is greater 10 395 µl: CV 3.0% or SD 0.4 µl, whichever is greater
5 µl: 10% (typical) 20 µl: 1.5% (typical) 100 µl: 1% (typical) Valid when counted for the entire plate

• using the calibration procedure in section 7.5 the accuracy can be improved to ± 1.5%

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

60

Chapter 8: Technical Specifications

8.2 Safety specifications
8.2.1 Live parts
The instrument is safe to operate with the covers fitted and these must not be removed during operation. The covers protect the user from live parts and they should only be removed after switching the instrument off and disconnecting the mains supply cable, and only by suitably qualified maintenance and repair personnel.
Voltages dangerous for human beings are present in this instrument. Before removing any covers, disconnect the instrument from the power supply.

The Multidrop 384 fulfils the following requirements:

EN 61010-1:1993 + A2:1995/IEC 61010-1:1990 + A1:1992 + A2:1995 taking into account US and CA National differences EN 61010-1:2001 (Ed. 2)

The safety specifications are also met under the following environmental conditions in addition to or in excess of those stated in the operating conditions:

Altitude Temperature Mains supply fluctuations Installation category (overvoltage category) Pollution degree

Up to 2000 m +10°C +40°C ± 10% from nominal II according to IEC 60664-1 (Note

1. 2 according to IEC 60664-1 (Note 2)

Notes

1. The installation category (overvoltage category) defines the level of transient overvoltage, which the instrument is designed to withstand safely. It depends on the nature of the electricity supply and its means of overvoltage protection. For example, in CAT II, which is the category used for instruments in installations supplied from a supply comparable to public mains, such as hospital and research laboratories and most industrial laboratories, the expected transient overvoltage is 2500 V for a 230 V supply and 1500 V for a 120 V supply.

2. The pollution degree describes the amount of conductive pollution present in the operating environment. Pollution degree 2 assumes that normally only nonconductive pollution, such as dust, occurs with the exception of occasional conductivity caused by condensation.

Both of these affect the dimensioning of the electrical insulation within the instrument.

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

61

Chapter 8: Technical Specifications

8.3 In conformity with the requirements

The Multidrop 384 bears the following markings:

Type 832 100 120 Vac, 200 240 Vac 50/60 Hz, 50 VA CE mark CSA monogram

The Multidrop 384 conforms to the following requirements:

2006/95/EC (Low Voltage Directive) 2004/108/EC (Electromagnetic Compatibility Directive, EMC) FCC Part 15, Subpart B/Class B 2002/96/EC (Waste of Electrical and Electronic Equipment)

Safety performance:

EN 61010-1:1993 + A2:1995/IEC 61010-1:1990 + A1:1992 + A2:1995 taking into account US and CA National differences EN 61010-1:2001 (Ed. 2)

EMC performance:

EN 50081-1:1992

Generic emission standard. Residential, commercial and light industry.

EN 50082-1:1997

Generic immunity standard. Residential, commercial and light industry.

EN 61326-1:1997 + A1:1998 Product family standard.

+ A2:2001 + A3:2003

Test standards and

Performance limits

EN 55022:1998 EN 61000-3-2:1995 + A1:1998 + A2:1998 + A13:1997 + A14:2000 EN 61000-3-3:1995 ANSI C63.4:1992

Class B, 150 kHz 1 GHz Class A Class B, 450 kHz ­ 1 GHz

EN 61000-4-2:1995 + A1:1998 EN 61000-4-3:1996 + A1:1998 EN 61000-4-4:1995 EN 61000-4-5:1995 EN 61000-4-6:1996 EN 61000-4-8:1994 EN 61000-4-11:1994

4 kV CD, 8 kV AD, Criteria B 3 V/m, 80 MHz 2 GHz, Criteria A 1 kV, Criteria B 2 kV line to ground, 1 kV line to line, Criteria B 3 Vrms, 150 kHz 80 MHz, Criteria A 3 A/m, Criteria A 30%/10 ms, Criteria B 60%/100 ms, Criteria C 100%/20 ms, Criteria B > 95%/5 s, Criteria C

Thermo Scientific Multidrop® 384 User Manual; Rev. 3.4, Cat. no. 1507010

62

Chapter 8: Technical Specifications

8.4 Remote control to Multidrop 384
The interface system consists of the serial RS-232C computer port which is used for computer control.

8.4.1 Hardware for the computer port

The character length is 10 bits with 1 start bit, 8 data bits, 1 stop bit and no parity. The format cannot be changed.
The following signals are connected to the computer port DB-25 connector at the rear of the instrument:

DB-25 CONNECTOR Pin no. 2 3 4 7 20

Signal name TxD RxD RTS GND DTR

Signal description

2: 3: 7: 4/20:

TxD, transmit data (output) RxD, receive data (input) GND, signal ground RTS/DTR (handshake lines)

The handshake lines: RTS/DTR are not in use in the standard Multidrop 384.

8.4.2 Multidrop 384 PC interface

Baudrate Parity Data bits Stop bits Handshake

9600 None 8 1 XON/XOFF

Commands
Commands to the Multidrop 384 have two to three fields as described in the following syntax. The first letter identifies the command. Then a numeric argument field is optional for some and required for other commands. The numeric field immediately follows the uppercase letter without any separating characters. The last character of a command is a linefeed character or a carriage return character . Commands may also be terminated to or