Thermo SCIENTIFIC Evolution Pro Spectrophotometers User Guide

Thermo SCIENTIFIC Evolution Pro Spectrophotometers

Evolution Pro – Getting Started

This guide gives an overview of common tasks that can be performed with the spectrophotometer. For more information, see the Evolution Pro User Guide or Insight Pro Help.

Spectrophotometer Components

Computer Control

To set up a Computer Control instrument

1. Turn on the computer.
2. Install Insight Pro™ software.
3. Connect the instrument to the computer using a USB cable.
4. Turn on the power switch on the left side of the spectrophotometer. Wait for the system to initialize. The power indicator on the front panel stops blinking when initialization is complete.
5. Double-click the Insight Pro desktop icon to start the software.

Insight Pro Window

The Insight Pro – Classic view displays view options along the top row, Application buttons in the center, and various functions along the bottom ribbon. Classic view is the default view of the home page.

Bottom Ribbon

The bottom ribbon provides access to the following:

Button  Description

• Experiment parameters can be saved as templates for later use.
• Stores records of experiments you have run.
• Run performance verification tests from Thermo Scientific, USP 2019, and EP lists.
• Perform wavelength and accessory calibrations, as well as beam alignment.
• The Settings feature on the Insight Pro page includes Applications, Reports, Preferences, Data, and Formulas & Units.
• Launches Thermo Fisher online help.

Applications

Fixed

• Measures light passing through the sample at one or more wavelengths. Click the Fixed button in Insight Pro – Classic view.

Scan

• Measures light that passes through the sample over a range of wavelengths. Click the Scan button in Insight Pro – Classic view.

Quant

• Use this to set up and perform quantitative analyses of sample data. Click the Quant button in Insight Pro – Classic view.

Live Display

• Used for quick measurements and simplified data collections in Fixed or Scan mode. Click Live Display in Insight Pro – Classic view.

Kinetics

• Use this to make time- or temperature-based kinetics measurements. Click the Kinetics button in Classic view.

Note Bio methods are displayed in Bio Methods view.

Common Tasks

Start the Insight Pro software, display the Home screen, and select the Classic group before beginning the first task.

Data Acquisition

To measure samples

1. Click an application button, such as Scan.
   This will open the setting page for that Application.

2. Set the desired parameters for the experiment.

3. Click and follow the instructions that appear.

4. Click and follow the instructions, providing information or
   installing a sample, or standard. The acquired data will appear:

Reports

An application that contains measurement results must be selected before you can create a report.

To create and print a report

1. Click .
2. Use the Reportable Data tab to specify the measurement results to include in the report.
3. Use the Layout tab to design the report.
4. Use the Samples tab to select the sample data to include.
5. Click

Data Storage

To open saved data and the associated application

1. Click from the Bottom Ribbon of the Insight Pro window.
2. Double-click the desired experiment from the list.

Instrument Performance

To verify instrument performance

1. Click from the Bottom Ribbon of the Insight Pro window.
2. Select the tests to run. Some tests require reference materials. If using an accessory, see its documentation for instructions.
3. Click .
4. Follow the instructions that appear. Test results are saved and appear in a window from which they can be printed or copied. They can also be accessed later on the
   PV Reports tab in History.

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