HACH DR1300 FL Portable Fluorometer User Manual

HACH DR1300 FL Portable Fluorometer

Section 1 Legal information

Manufacturer: Pyxis Lab, Inc. Distributor: Hach Company The translation of the manual is approved by the manufacturer.

Section 2 Specifications

Specifications are subject to change without notice.

non-condensing
Specification| Details
---|---
Storage temperature| -18 to 60 °C (0 to 140 °F)
Altitude| 3000 m (9843 ft) maximum
Battery life| 3 months
Sample cell| 16-mm round cell
Data storage| 2 years; 60,000,000 data points maximum
Data transfer| Built-in Bluetooth®1 (not available in some countries) Desktop app for data transfer to a PC with the Bluetooth/USB adapter
Integrated Bluetooth Low Energy Module (instrument) and Bluetooth/USB adapter2| Ehong Bluetooth® Low Energy Module – Model: EH-MC17 Contains transmitter module:

• FCC ID: 2ACCRMC17
• IC: 12208A-04
• CE Compliant per EU Radio Equipment – Directive (RED) 2014/53/EU

The Integrated Bluetooth Low Energy Module (instrument) and Bluetooth/USB adapter are approved for installation and use in mobile and/or portable host platforms.

Certifications| CE, UKCA, FCC/ISED, RCM, KC
Warranty| 1 year (EU: 2 years)

Section 3 General information

In no event will the manufacturer be liable for damages resulting from any improper use of product or failure to comply with the instructions in the manual. The manufacturer reserves the right to make changes in this manual and the products it describes at any time, without notice or obligation. Revised editions are found on the manufacturer’s website.

1. The Bluetooth® word mark and logos are registered trademarks owned by the Bluetooth SIG, Inc. and any use of such marks by HACH is under license.
2. Only supplied with instruments with the Bluetooth option.

Safety information

The manufacturer is not responsible for any damages due to misapplication or misuse of this product including, without limitation, direct, incidental and consequential damages, and disclaims such damages to the full extent permitted under applicable law. The user is solely responsible to identify critical application risks and install appropriate mechanisms to protect processes during a possible equipment malfunction.
Please read this entire manual before unpacking, setting up or operating this equipment. Pay attention to all danger and caution statements. Failure to do so could result in serious injury to the operator or damage to the equipment.
Make sure that the protection provided by this equipment is not impaired. Do not use or install this equipment in any manner other than that specified in this manual.

Use of hazard information

D A N G E R

Indicates a potentially or imminently hazardous situation which, if not avoided, will result in death or serious injury.

W A R N I N G

Indicates a potentially or imminently hazardous situation which, if not avoided, could result in death or serious injury.

C A U T I O N

Indicates a potentially hazardous situation that may result in minor or moderate
injury

N O T I C E

Indicates a situation which, if not avoided, may cause damage to the instrument. Information that requires special emphasis

Precautionary labels

Read all labels and tags attached to the instrument. Personal injury or damage to the instrument could occur if not observed. A symbol on the instrument is referenced in the manual with a precautionary statement.

Electrical equipment marked with this symbol may not be disposed of in European domestic or public disposal systems. Return old or end-of-life equipment to the manufacturer for disposal at no charge to the user.

Certification

Canadian Radio Interference-Causing Equipment Regulation, ICES-003, Class B:

Supporting test records reside with the manufacturer.
This Class B digital apparatus meets all requirements of the Canadian Interference-Causing Equipment Regulations.

FCC Part 15, Class “B” Limits

Supporting test records reside with the manufacturer. The device complies with Part 15 of the FCC Rules. Operation is subject to the following conditions:

1.  The equipment may not cause harmful interference.
2.  The equipment must accept any interference received, including interference that may cause undesired operation.

Changes or modifications to this equipment not expressly approved by the party responsible for compliance could void the user’s authority to operate the equipment. This equipment has been tested and found to comply with the limits for a Class B digital device, pursuant to Part 15 of the FCC rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment. This equipment generates, uses and can radiate radio frequency energy and, if not installed and used in accordance with the instruction manual, may cause harmful interference to radio communications. Operation of this equipment in residential area is likely to cause harmful interference, in which case the user will be required to correct the interference at their expense. The following techniques can be used to reduce interference problems:

1.  Move the equipment away from the device receiving the interference.
2.  Reposition the receiving antenna for the device receiving the interference.
3.  Try combinations of the above.

Intended use

The Hach DR1300 FL is intended for use by water treatment professionals who need to make sure that there is a consistently-low level of free and total chlorine in process water. The DR1300 FL is used to monitor free and total chlorine levels to prevent damage to product quality and prevent equipment damage caused by chlorine.
In addition, the DR1300 FL is intended for use by water treatment professionals who use sodium bisulfite for dichlorination. The DR1300 FL is used to monitor sulfite at the end of the dichlorination process to make sure that the dichlorination process is efficient.

Product overview

N O T I C E

Keep magnetic sensitive devices a minimum of 2 inches from the sample port cover to prevent damage or data loss.

The DR1300 FL fluorometer measures ultra low-range total chlorine, free chlorine and sulfite in water. Figure 1 gives an overview of the DR1300 FL fluorometer.
Note: This instrument has not been evaluated to measure chlorine or chloramines in medical applications in the United States

he DR1300 FL fluoric

1. Sample port cover3
2. Sample port
3. Keypad
4.  LCD display

Keep the sample port cover closed during transport, storage and startup.

Product components

Make sure that all components have been received. Refer to Figure 2. If any items are missing or damaged, contact the manufacturer or a sales representative immediately. Figure 2 Product components

1. DR1300 FL fluorometer
2. Vial cover
3. Sample vials (2x)
4. Vial adapter
5. Bluetooth/USB adapter4
6. Four AA alkaline batteries, nonchargeable

Only supplied with instruments with the Bluetooth option.

Section 4 Install the batteries

W A R N I N G

Explosion hazard. Incorrect battery installation can cause the release of explosive gases. Be sure that the battery is of the approved chemical type and is inserted in the correct orientation.

Install the four supplied AA alkaline batteries. Refer to Figure 3.

Figure 3 Install the batteries

Section 5 User interface and navigation

Keypad

Figure 4 shows the keypad and gives the key functions.

Figure 4 Keys

1. Enter key—Sets the instrument to on. Push for 5 seconds to set the instrument to off. Confirms the selection.
2. Left arrow key—Moves the cur sorbet.
3. Up arrow key—Moves the cursor up or increases the value.
4. Right arrow key—Moves the cursor right.
5. Down arrow key—Moves the cursor down or decreases the value.

Display

Figure 5 shows the main menu.

Figure 5 Main menu

1. SYSTEM—Sets the instrument settings and shows diagnostics information.
2. TESTS—Shows the test options.
3. Date (month, day and year)
4. Time (hours and minutes)
5. Battery power level
6. CALIBRATION—Shows the calibration options.
7. POWER—Sets the instrument to off.

Section 6 Configure the system settings

Set the date, time, display backlight and power off time settings at the instrument.

1.  Push Enter to set the instrument to on.
2.  Select SYSTEM > System Settings.
3.  Push the UP and DOWN arrows to select an option, then push Enter to change the setting.

Option

| Description
---|---

Language

| Changes the displayed language (default: English).
Note: Push the UP arrow to change the setting. Push Enter to save the changes.

Screen off time

| Sets the inactivity period after which the display backlight is
set to off if no keys are pushed. Default: 30 seconds.
Note: Push the UP and DOWN arrows to change the setting. Push Enter to save the changes.

Auto Power off time

| Sets the inactivity period after which the instrument power is set to off if no keys are pushed, except during a measurement. Default: 60 seconds.
Note: Push the UP and DOWN arrow to change the setting.
Push Enter to save the changes.

Date & Time

| Sets the date and time.

1. Push the UP and DOWN arrows to select the date format.
   Options: MM-DD-YYYY (default), YYYY-MM-DD or DDMM-YYYY with “-“, “.” or “/” as separator

2. Push the RIGHT arrow.

3.  Push the UP and DOWN arrows to set the time format (12-hour or 24-hour format). Options: 24H HH:MM (default), 24H HH:MM:SS, 12H HH:MM or 12H HH:MM:SS

4.  Push the RIGHT arrow.

5.  Set the date (year – month – day).

6.  Set the time (hour : minute : second).

7.  Push the RIGHT arrow to select OK.

8.  Push Enter to save the changes.

settings.
Factory| For Service use only
Exit| Goes back to the previous menu.

Section 7 Do a test

D A N G E R

Chemical or biological hazards. If this instrument is used to monitor a treatment process and/or chemical feed system for which there are regulatory limits and monitoring requirements related to public health, public safety, food or beverage manufacture or processing, it is the responsibility of the user of this instrument to know and abide by any applicable regulation and to have sufficient and appropriate mechanisms in place for compliance with applicable regulations in the event of malfunction of the instrument.

C A U T I O N

Chemical exposure hazard. Obey laboratory safety procedures and wear all of the personal protective equipment appropriate to the chemicals that are handled. Refer to the current safety data sheets (MSDS/SDS) for safety protocols.

C A U T I O N

Chemical exposure hazard. Dispose of chemicals and wastes in accordance with local, regional and national regulations.

To do a test for a specific parameter (e.g., total chlorine), download the test procedure from the manufacturer’s website. Refer to Download a test procedure on page 15.

The generic steps to do a test follow.

1.  Push Enter to set the instrument to on. Keep the sample port cover closed during startup.

2.  Select TESTS, then select the parameter to measure.

3. Measure a water sample as follows:
   a. Prepare the sample. Refer to the test procedure. Note: The sample vial holder is supplied with the test kits’. Select TIMER > START to start the timer, if applicable.
   c. When the timer expires, clean the external surface of the sample vial with a no-lint cloth.
   d. Install the vial adapter in the instrument. Refer to the illustrated steps in Install the vial adapter, vial and vial cover on page 15.
   e. Install the sample vial in the vial adapter.
   f. Put the vial cover on the sample vial.
   g. Select BACK > READ.

4. . Immediately rinse the sample vial and cap three times with deionized water (or distilled water). Keep the caps on the sample vials when not in use.

Install the vial adapter, vial and vial cover

Download a test procedure

1.  Go to http://www.hach.com.
2.  Enter “DR1300 FL” in the Search box.
3.  Select the “Downloads” option on the left side in the “Search Type” box.
4.  Scroll down to “Methods/Procedures”.
5.  Click the link for the applicable test procedure to download it.

Section 8 Calibration

C A U T I O N

Chemical exposure hazard. Obey laboratory safety procedures and wear all of the personal protective equipment appropriate to the chemicals that are handled. Refer to the current safety data sheets (MSDS/SDS) for safety protocols.

Do a reagent blank calibration

Do a reagent blank calibration before a set of new reagent bottles is used.

1.  Push Enter to set the instrument to on. Keep the sample port cover closed during startup.

2.  After startup, make sure that the sample port is clean. Use a soft cloth or no-lint paper tissue (or air duster) to clean the sample port as necessary.

3.  Install the vial adapter in the instrument. Push in the tabs on the sides of the vial adapter. Refer to the illustrated steps in Install the vial adapter, vial and vial cover on page 15.

4.  Select CALIBRATION, then select the parameter for the calibration.

5.  Select CAL-RB.

6.  Measure a reagent blank as follows:
   a. Prepare a reagent blank. Replace the sample with deionized water (or distilled water or organic-free water) in the test procedure. Do not use the timer.
   b. Clean the external surface of the sample vial with a no-lint cloth.
   c. Install the sample vial in the vial adapter.
   d. Put the vial cover on the sample vial.
   e. Push Enter to measure the reagent blank.

7.  Push Enter to save the results.

8. Immediately rinse the sample vial and cap three times with deionized water (or distilled water). Keep the caps on the sample vials when not in use

Do a calibration check

Use the DR1300 FL Fluorescence Standards Kit to do a calibration check at regular intervals. Refer to Consumables and replacement parts on page 25 for ordering information.

1.  Make sure that the DR1300 FL Fluorescence Standards Kit is within the expiration date.

2.  Get the Certificate of Analysis for the DR1300 FL Fluorescence Standards Kit.
   The Certificate of Analysis is available at https://app.hach.com/coaweb/customer_coa_request.asp.

3.  Push Enter to set the instrument to on. Keep the sample port cover closed during startup.

4.  After startup, make sure that the sample port is clean. Use a soft cloth or no-lint paper tissue (or air duster) to clean the sample port as necessary.

5.  Install the vial adapter in the instrument. Push in the tabs on the sides of the vial adapter. Refer to Install the vial adapter, vial and vial cover on page 15.

6. Select CALIBRATION, then select the parameter to measure.

7.  Select CALIBRATION CHECK.

8.  Measure deionized water as follows:
   a. Fill a sample vial with deionized water.
   b. Clean the external surface of the sample vial with a no-lint cloth.
   c. Install the sample vial in the vial adapter.
   d. Put the vial cover on the sample vial.
   e. Push Enter to select ZERO.

9. Measure the Standard 1 vial as follows:
   a. Clean the Standard 1 vial with a no-lint cloth.
   b. Install the Standard 1 vial in the vial adapter.

10. Measure the Standard 2 vial as follows:
    a. Clean the Standard 2 vial with a no-lint cloth.
    b. Install the Standard 2 vial in the vial adapter.
    c. Put the vial cover on the sample vial.
    d. Push Enter to select READ.

11. Identify if a slope calibration is necessary as follows:

• Total chlorine—If the Standard 1 reading is not within ±2 ppb of the total chlorine value from the Certificate of Analysis and the Standard 2 reading is not within ±5 ppb of the total chlorine value from the Certificate of Analysis, do a slope calibration for total chlorine.
• free chlorine—If the Standard 1 reading is not within ±2 ppb of the free chlorine value from the Certificate of Analysis and the Standard 2 reading is not within ±5 ppb of the free chlorine value from the Certificate of Analysis, do a slope calibration for free chlorine.
• Sulfite—If the Standard 1 reading is not within ±5 ppb of the sulfite value from the Certificate of Analysis and the Standard 2 reading is not within ±20 ppb of the sulfite value from the Certificate of Analysis, do a slope calibration for sulfite.

Do a slope calibration

Do a 2-point slope calibration only if the instrument does not pass a calibration check.

1.  Push Enter to set the instrument to on. Keep the sample port cover closed during startup.

2.  After startup, make sure that the sample port is clean. Use a soft cloth or no-lint paper tissue (or air duster) to clean the sample port as necessary.

3.  Install the vial adapter in the instrument. Push in the tabs on thesides of the vial adapter.

4.  Select CALIBRATION, then select the parameter for the calibration. Select CAL-SLP.

5. Measure the Standard 1 vial as follows:
   a. Enter the concentration for the Standard 1 vial (ppb) from the Certificate of Analysis for the parameter selected in step 4.
   b. Clean the Standard 1 vial with a no-lint cloth.
   c. Install the Standard 1 vial in the vial adapter.
   d. Put the vial cover on the sample vial.
   e. Push Enter to measure the Standard 1 vial.

6. Measure the Standard 2 vial as follows:
   a. Enter the concentration for the Standard 2 vial (ppb) from the Certificate of Analysis for the parameter selected in step 4.
   b. Clean the Standard 2 vial with a no-lint cloth.
   c. Install the Standard 2 vial in the vial adapter.
   d. Put the vial cover on the sample vial.
   e. Push Enter to measure the Standard 2 vial

7. Push Enter to save the results.

Set to the factory default calibration

To remove a user-entered slope calibration from the instrument and use the factory slope calibration, do the steps that follow:

1.  Select CALIBRATION, then select the parameter for the calibration.
2.  Select DEFAULT, then push Enter to confirm.

Section 9 Data management

Show the measurements on the instrument

1.  Select SYSTEM > Log.
2.  Select the month the measurements were done, then push Enter.
3.  Push the DOWN and UP arrows to scroll through the
   measurements.

Show and save the measurements on a PC

Show and save the measurements on a PC with the desktop app and Bluetooth/USB adapter.

Note: The instrument must have the Bluetooth option to use the desktop app.

1. Download the desktop app to the PC as follows:
   a. Go to http://www.hach.com.
   b. Enter “DR1300 FL” in the Search box.
   c. Select the “Downloads” option on the left side in the “Search Type” box.
   d. Scroll down to “Software”.
   e. Click the “DR1300 FL Data Management Tool” link.

2. Install and start the desktop app.

3. Install the supplied Bluetooth/USB adapter5 in the PC.

4.  On the instrument, select SYSTEM > BTLE to set Bluetooth to on.
   Note: Bluetooth is set to off when Exit is selected or the instrument is set to off.

5.  On the desktop app, select Device > Connect via USB-Bluetooth at the top of the window. A new window opens.

6.  Select DR1300 FL, then click Connect to Device.

7.  Select Data log  > Read  Data log List. A list of dates shows on the display.

8.  Select the log. Then, select Read Data log  to show the datalog.

9.  Select Export as CSV File to save the datalog to the PC.

Section 10 Maintenance

Replace the batteries

WARNING

Explosion hazard. Incorrect battery installation can cause the release of explosive gases. Be sure that the battery is of the approved chemical type and is inserted in the correct orientation.

Only supplied with instruments with the Bluetooth option.

CAUTION

Fire hazard. Do not mix batteries from different manufacturers.
Dispose of batteries in accordance with local, regional and national regulations.

When “LOW BATTERY” shows on the display, replace the four AA alkaline batteries. Refer to Install the batteries on page 10. Use only non- rechargeable batteries.

Clean the sample port

Keep the sample port clean and dry. Debris and liquids in the measurement cell can change the accuracy of the instrument.
Use a soft cloth or no-lint paper tissue (or air duster) to clean the sample port as necessary.

Clean the instrument

Clean the exterior of the instrument with a moist cloth and a mild soap solution and then wipe the instrument dry as necessary.

Section 11 Troubleshooting

If an error occurs, a warning message will show on the display with instructions.
If the instrument does not respond, remove the batteries and theninstall the batteries. Replace the batteries if the battery power level is low.
To do a diagnostic test, select SYSTEM > Diagnosis with the sample port cover closed. Supply the diagnostic data to technical support for analysis

correct orientation.

The battery power level is low.

| Make sure the battery orientation is correct.

Replace the batteries if the battery power level is low. Use only non- rechargeable batteries.

The chlorine readings are low.| The sample containers, sample vials, caps and other items that touched the sample have a chlorine demand.| Collect samples in clean glass bottles. Do not use plastic containers. Plastic containers can have a large chlorine demand.

Pretreat items that the sample may touch to remove chlorine demand. Refer to the instructions in the Sample Collection section of the test procedure.

The chlorine readings are low.| The sample was not analyzed immediately.| Analyze the samples immediately. Chlorine is a strong oxidizing agent and is unstable in natural waters.
The total chlorine readings are high or low.| The reagents were added in an incorrect sequence.| Make sure that the reagents are added to the sample vial in the sequence given in the test procedure.
The readings are low.| The reagent blank value is not correct or missing.| Make sure to do a reagent blank calibration before a set of new reagent bottles is used. Refer to Do a reagent blank calibration

If the problem continues, do a calibration check. Refer to Do a calibration check on page 17.

The readings are low.| The reagent bottle was not closed immediately after use. The solvent in the reagent evaporated or the reagent was contaminated.| Discard the set of reagent bottles. Use a new set of reagent bottles. Do a reagent blank calibration. Refer to Do a reagent blank calibration

Close the reagent bottles immediately after use.

The readings are low.| The quantity of reagent added was not correct. The reagent was not correctly dispensed.| Hold the reagent bottle vertically above the sample vial when reagent is added.

Note: If the reagent bottle is horizontal when drops are added, the quantity of reagent added will change.

The readings are low.| The reagents are no longer good.| Discard the set of reagent bottles. Use a new set of reagent bottles. Do a reagent blank calibration. Refer to Do a reagent blank calibration

Keep the new reagents bottles out of direct sunlight or indoor UV illumination. UV light causes damage to the reagents. When not in use, keep the reagent bottles in the dark.

The readings are low.| The sample port is dirty.| Clean the sample port. Refer to Clean the sample port on page 21.
The readings are low.| The sample vials are dirty or have scratches.| Clean the sample vials if dirty. Replace the sample vials if the sample vials have scratches.
The readings are low.| The wrong parameter was selected.| Make sure to select the correct parameter (e.g., free chlorine) and use the correct reagents for the parameter.
The readings are low.| The reagents have expired.| Discard the set of reagent bottles. Use a new set of reagent bottles. Do a reagent blank calibration. Refer to Do a reagent blank calibration
The readings are high.| The reagent blank value is not correct or missing.| Make sure to do a reagent blank calibration before a set of new reagent bottles is used. Refer to Do a reagent blank calibration

If the problem continues, do a calibration check. Refer to Do a calibration check on page 17.

The readings are high.| The sample vials are dirty or have scratches.| Clean the sample vials if dirty. Replace the sample vials if the sample vials have scratches.
The readings are high.| The wrong parameter was selected.| Make sure to select the correct parameter (e.g., free chlorine) and use the correct reagents for the parameter.
Unexpected readings| The sample is not a clean water sample.| The DR1300 FL is intended for use with clean water samples.
The instrument reading is different from the online analyzer reading.| The sample is not a representative sample and is not well mixed.| Make sure to get a representative sample. Refer to the instructions in the Sample Collection section of the test procedure.
The instrument reading is different from the online analyzer reading.| The grab sample was not analyzed immediately.

Note: The DPD method is less accurate in the lower ppb range compared to the fluorescence method.
Other oxidants do not interfere with the fluorescence method.

| Analyze the grab samples immediately. The chlorine concentration decreases quickly in ultra low range chlorine samples.

Section 12 Consumables and replacement parts

WARNING

Personal injury hazard. Use of non-approved parts may cause personal injury, damage to the instrument or equipment malfunction. The replacement parts in this section are approved by the manufacturer

Note: Product and Article numbers may vary for some selling regions. Contact the appropriate distributor or refer to the company website for contact information

Consumables

Reagent kit, ULR Fluorescence Total Chlorine, includes:
Sample vial holder, two16-mm vials with caps and two reagent bottles in dropper bottles| 34252000
Reagent kit, ULR Fluorescence Free Chlorine, includes: Sample vial holder, two16-mm vials with caps and two reagent bottles in dropper bottles| 34251000
Reagent kit, ULR Fluorescence Sulfite, includes: Sample vial holder, two16-mm vials with caps and two reagent bottles in dropper bottles| 34250000
---|---
Refill kit, ULR Fluorescence Total Chlorine Reagent, 100 tests, includes:

Two reagents in dropper bottles

| 34252001
Refill kit, ULR Fluorescence Free Chlorine Reagent, 100 tests, includes:
Two reagents in dropper bottles| 34251001
Refill kit, ULR Fluorescence Sulfite Chlorine Reagent, 100 tests, includes:
Two reagents in dropper bottles| 34250001

Replacement parts

The instrument must have the Bluetooth option to use the Bluetooth/USB adapter.

References

• Geräte und Reagenzien für die Wasserqualitätsanalyse | Hach
• Hach | Hach
• Hach Certificate of Analysis Online Request Form

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