MACHEREY-NAGEL 985003 NANOCOLOR Ammonium 3 Tube Test Instructions
- June 3, 2024
- MACHEREY-NAGEL
Table of Contents
MACHEREY-NAGEL 985003 NANOCOLOR Ammonium 3 Tube Test
Overview
The test is suitable for the photometric determination of NH4+. The test is in
accordance with APHA 4500-NH3 F, ISO 7150-1, DIN 38406-E5 and EPA 350.1.
The test is suitable for surface water, groundwater and drinking water and
slightly contaminated wastewater.
Results are highly reproducible in water with low levels of pollutants.
- Measuring range:
- 0.04–2.30 mg/L NH4-N (method 0031)
- 0.05–3.00 mg/L NH4+ / NH3 (method 0032 / 0033)
- Number of tests: 20
- Wavelength for photometric determination: 660 / 690 nm
- Shelf life: 12 months
- Reaction time: 15 minutes
- Storage temperature: 15–25 °C
- Storage conditions: upright
Method
Photometric determination of a blue indophenol dye based on a reaction with hypochlorite and salicylate at a pH of 12.6 in the presence of sodium nitroprusside.
Interferences
Heavy pollution results in errors and requires prior distillation.
The method is suitable for the analysis of seawater after 1+1 dilution.
Turbidities cause higher measurement values.
Reagents and accessories
Contents of reagents set:
- 20 test tubes R0
- 1 NANOFIX R2
Required devices:
- MACHEREY-NAGEL photometer
- Digital piston pipette 1–5 mL (REF 916909) with pipette tips (REF 916916)
- Tweezers for sampling NANOFIX capsules (REF 916114)
Standards
NANOCONTROL Multistandard Sewage outflow 2 (REF 925010)
Sampling and preparation
See DIN EN ISO 5667-3-A 21.
Adjust to pH 7–10 with NaOH or HCl prior to analysis.
Adjust highly buffered solutions to pH 9–10.
Quality control
The measurement of a blank value and a standard is recommended before every
measuring series as quality control measure.
Quality data:
The following data were determined during production according to ISO 8466-1
and DIN 38402-A51:
- Number of LOTs: 40
- Standard deviation of the method: ± 0.02 mg/L NH4-N
- Coefficient of variation of the process: ± 1.60 %
- Confidence interval: ± 0.04 mg/L NH4-N
Specified data for procedure:
- Sensitivity (absorbance of 0.010 A corresponds to): 0.013 mg/L NH4-N
- Accuracy of a measurement value: ± 0.03 mg/L NH4-N LOT-specific certificates are available at www.mn-net.com.
Procedure
- Open test tube
- Pipette 4 mL of sample into test tube
- Add 1 NANOFIX R2
- Seal test tube and shake vigorously
- Wait 15 min
- Clean outside of test tube
- Measure
Notes
When using other photometers, make sure measurements are possible in test
tubes (16 mm OD) and calibrate the method.
Correction value e. g. for colored or turbid samples possible (see photometer
manual).
Information regarding safety can be found on the box’ label and in the safety
data sheet. You can download the SDS from www.mn-net.com/SDS.
MACHEREY-NAGEL GmbH & Co. KG · Neumann-Neander-Str. 6–8 · 52355 Düren ·
Germany
Tel.: +49 24 21 969-0
Fax: +49 24 21 969-199
E-mail: info@mn-net.com