MACHEREY-NAGEL 985008 NANOCOLOR Ammonium 100 Tube Test Instructions
- June 3, 2024
- MACHEREY-NAGEL
Table of Contents
NANOCOLOR®Ammonium 100
REF: 985008
Overview
The test is suitable for the photometric determination of NH4 + .
The test is in accordance with APHA 4500-NH3 F, ISO 7150-1, DIN 38406-E5
and EPA 350.1.
The test is suitable for surface water, ground and drinking water.
- Measuring range: 4–80 mg/L NH4-N 5–100 mg/L NH4 + / NH3
- Number of tests: 20
- Wavelength for photometric determination: 585 nm
- Shelf life: 12 months
- Reaction time: 15 minutes
- Storage temperature: 15–25 °C
- Storage conditions: upright
Method
Photometric determination of a blue indophenol dye based on a reaction with
hypochlorite and salicylate at a pH of 12.6 in the presence of sodium
nitroprusside.
Interferences
The method can be applied for analyzing seawater. Turbidities cause higher
measurement values.
Reagents and accessories
Contents of reagents set:
- 20 test tubes R0
- 1 reagent R2
Required devices:
- MACHEREY-NAGEL photometer
- Digital piston pipette 100–1000 µL (REF 91677) with pipette tips (REF 91667)
- Tweezers for sampling NANOFIX capsules (REF 916114)
Standards
- NANOCONTROL Multistandard Sewage (REF 925013)
Sampling and preparation
See DIN EN ISO 5667-3-A 21.
Adjust to pH 1–13 with NaOH or HCl prior to analysis.
Quality control
The measurement of a blank value and a standard is recommended before every
measuring series as quality control measure.
Quality data:
The following data were determined during production according to ISO 8466-1
and DIN 38402-A51:
- Number of LOTs: 27
- Standard deviation of the method: ± 0.85 mg/L NH4-N
- Coefficient of variation of the process: ± 2.01 %
- Confidence interval: ± 1.95 mg/L NH4-N
Specified data for procedure:
- Sensitivity (absorbance of 0.010 A corresponds to): 0.5 mg/L NH4-N
- Accuracy of a measurement value: ± 0.146 mg/L NH4-N LOT-specific certificates are available at www.mn‑net.com.
Procedure
- Open test tube
- Pipette 500 µL of sample into test tube
- Add 1 NANOFIX R2
- Seal test tube and shake vigorously
- Wait 15 min
- Clean outside of test tube
- Measure
Notes
When using other photometers, make sure measurements are possible in test
tubes (16 mm OD) and calibrate the method.
Correction value e. g. for colored or turbid samples possible (see photometer
manual).
Information regarding safety can be found on the box’ label and in the safety
data sheet. You can download the SDS from
www.mn‑net.com/SDS. 08/2020
MACHEREY-NAGEL GmbH & Co. KG · Neumann-Neander-Str. 6–8 · 52355 Düren · Germany
DE / International:
Tel.: +49 24 21 969-0
Fax: +49 24 21 969-199
E-mail:info@mn-net.com| CH:
Tel.: +41 62 388 55 00
Fax: +41 62 388 55 05
E-Mail: sales-ch@mn-net.com| FR:
Tel.: +33 388 68 22 68
Fax: +33 388 51 76 88
E-Mail:sales-ch@mn-net.com
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