MACHEREY-NAGEL Test 0-74 NANOCOLOR Phenolic index 5 Tube Test Instructions

MACHEREY-NAGEL Test 0-74 NANOCOLOR Phenolic index 5 Tube Test

Method

Photometric determination of phenols and other compounds capable of oxidative coupling, which forms antipyrine dyes with 4-amino antipyrine, and, if wanted, subsequent extraction with isobutyl methyl ketone (MIBK).

index
Wavelength|  |
(HW = 5–12 nm):| 520 nm| 445 nm             470 nm
Reaction time:| 5 min (300 s)| 15 min (900 s)
Reaction temperature:| 20–25 °C| 20–25 °C

Contents of reagent set

• 20 test tubes Phenolic index 5
• 2 test tubes each with 11 mL Phenolic index 5 R2
• 1 tube NANOFIX Phenolic index 5 R3

Note:
The content of the 20 test tubes with Phenolic index 5 may be colored light yellow, which has no influence on the color reaction.

Hazard warning

Test tubes contain 4-amino antipyrine 25–100 %, reagent R2 contains ammonia 1–5 %, and reagent R3 contains potassium peroxodisulfate 20–100 %. H317 and H334 May cause an allergic skin reaction. May cause allergy or asthma symptoms or breathing difficulties if inhaled. P261, P272, P280, P302+352, P304+340, P333+313, P342+311, P363 Avoid breathing dust. Contaminated work clothing should not be allowed out of the workplace. Wear protective gloves/eye protection. IF ON SKIN: Wash with plenty of water / … IF INHALED: Remove person to fresh air and keep comfortable for breathing. If skin irritation or rash occurs: Get medical advice/attention. If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ … Wash contaminated clothing before reuse. For further information ask for a safety data sheet.

Interferences

• oxidizing substances
• reducing substances
• cyanides
• applicable for turbid water after extraction
• applicable for seawater after extraction

To ensure the measuring results, extraction with isobutyl methyl ketone (MIBK, REF 918 929) is recommended, analogous to DIN 38 409 H16.

Procedure

Requisite accessories: piston pipette with tips

Open the test tube, add

• 1.0 mL R2 and
• 4.0 mL test sample (the pH value of the sample must be between pH 1 and 13), close, and mix. Add

1 NANOFIX R3, close and mix.

• (Close the NANOFIX tube immediately after use.) Clean the outside of the test tube and measure after 5 min.

Procedure with extraction

Requisite accessories: piston pipette with tips, glass cuvettes 10 mm, isobutyl methyl ketone R4 (MIBK, REF 918 929).

Open the test tube, add

1.0 mL R2 and

4.0 mL test sample (the pH value of the sample must be between pH 1 and 13), close, and mix. Add

1 NANOFIX R3, close and mix.

(Close the NANOFIX tube immediately after use.)

Wait 5 min. Add

4.0 mL R4 (MIBK), close and shake vigorously for 30 s.

| Open test tube, add

1.0 mL R2 and

4.0 mL distilled water, close and mix.

Add

1 NANOFIX R3, close and mix.

(Close the NANOFIX tube immediately after use.)

Wait 5 min. Add

4.0 mL R4 (MIBK), close and shake vigorously for 30 s.

After 15 min open test tubes with the test sample and blank value and pipet each about 2 mL of the upper organic phase into 10 mm cuvettes and measure [method 1742].

Measurement

For NANOCOLOR® photometers and PF-12 see manual, test 0-74.

Measurement when samples are colored or turbid:
For all NANOCOLOR® photometers see the manual and use the key for correction value.

Photometers of other manufacturers:
For other photometers check whether the measurement of round glass tubes is possible. Verify the factor for each type of instrument by measuring standard solutions.

MACHEREY-NAGEL GmbH & Co. KG · Neumann-Neander-Str. 6–8 · 52355 Düren · Germany Tel.: +49 24 21 969-0 · Fax: +49 24 21 969-199 · info@mn- net.com. www.mn-net.com.

PD 14122 / A011703 / 985 074 / 0861.

References

• MACHEREY-NAGEL Webshop | MACHEREY-NAGEL

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