MACHEREY-NAGEL visocolor ECO Nitrate Test Kit User Manual

MACHEREY-NAGEL visocolor ECO Nitrate Test Kit

Test kit for performing colorimetric tests on nitrate ions in surface water and sewage.

Method

Nitrate ions are reduced to nitrite ions in an acidic medium. Combined with a suitable aromatic amine, these form an orange-yellow azo dye.

Measurement range

• 1–120 mg/L NO3–

Contents of test kit (refill pack)

sufficient for 110 tests

• 30 mL NO3-1*
• 5 g NO3-2*
• 1 measuring spoon 70 mm*
• 2 screw-plug measuring glasses
• 1 slide comparator
• 1 color chart
• 1 plastic syringe 5 mL
• 1 instruction for use

Hazard warning:
Information regarding safety can be found on the box label and in the safety data sheet. You can download the SDS from www.mn-net.com/SDS.

Instructions for use

Also, refer to the pictogram on the back of the color chart

1. Pour a 5 mL water sample into each of the measuring glasses using the plastic syringe. Place a measuring glass on position A in the comparator.
   Only add the reagent to measuring glass B.

2. Add 5 drops of NO3-1, seal the glass, and mix.

3. Add 1 level measuring spoonful of NO3-2, seal the glass and immediately shake the mixture well for 1 min.

4. Open the glass after 5 min and place it on position B in the comparator.

5. Slide the comparator until the colors match in the inspection hole on top. Check the measurement reading in the recess on the comparator reed. Mid-values can be estimated.

6. After use, rinse out both measuring glasses thoroughly and seal them.

The reagents can be used for the photometric evaluation with photo-meter PF-12 / PF-12Plus. This technique can be used also for analyzing seawater (see „Conversion table“).

Disposing of the samples:
Information regarding disposal can be found in the safety data sheet. You can download the SDS from www.mn-net.com/SDS.

Interferences

Depending on their concentration, oxidizing substances may reduce the measurement reading or suppress the reaction totally. Chlorine ≤ 10 mg/L does not interfere. Nitrite interferes (same reaction). This can be circumvented by the addition of amido sulphonic acid (REF 918 973). The water sample should be between 18 and 30 °C. At lower temperatures, the reaction takes place at a significantly slower rate, and the results are limited.

Conversion table

mg/L NO3 –

|

mg/L NO3-N (Nitrate nitrogen)

|

mmol/m3

|

mg/L NO3 – in seawater

---|---|---|---
1| 0.2| 16| 1
3| 0.7| 48| 3
5| 1.1| 81| 5
10| 2.3| 160| 12
20| 4.5| 320| 25
30| 6.8| 480| 40
50| 11| 810| 65
70| 16| 1130| 95
90| 20| 1450| 120
120| 27| 1940| 160

Storage:
Store the test kit in a cool (< 25 °C) and dry place.

MACHEREY-NAGEL GmbH & Co. KG · Valencienner Str. 11 · 52355 Düren · Germany.
Tel.: +49 24 21 969-0 · [email protected] · www.mn- net.com.

References

• MACHEREY-NAGEL Webshop | MACHEREY-NAGEL

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