MACHEREY-NAGEL 985043 NANOCOLOR Hardness 20 Tube Test User Manual

NANOCOLOR® Hardness 20
985043 Tube Test
User Manual

Overview

The test is suitable for the photometric determination of total hardness.
The test is suitable for water.

• Measuring range:
  1.0–20.0 mg/L °d (method 0431)
  0.2–3.6 mmol/L (method 0432)
  1–25 mg/L °e (method 0433)
  2–36 mg/L °f (method 0434)
  10–100 mg/L Ca 2+ (method 0435)
  5–50 mg/L Mg 2+ (method 5–50 mg/L Mg 2+
  20–350 mg/L CaCO3 (method 20–350 mg/L CaCO3

• Number of tests: 20

• Wavelengths for photometric determination: 540 / 570 nm

• Shelf life: 18 months

• Reaction time: 1 minute

• Storage temperature: 15–25 °C

• Storage conditions: upright

Method
Photometric determination of total hardness with phthalein purple.
The use of a selective masking agent permits a differentiation between calcium and magnesium.

Interferences

The following contaminants do not interfere with the test up to the indicated concentrations. The cumulative effect of different interfering  ions has not been tested.
Data in mg/L:
• Cu 2+ : 5
The method is suitable for the analysis of seawater after 1+29 dilution. Turbidities cause higher measurement values.
Concentrations above the double measuring range can simulate results within the measuring range and can therefore be misinterpreted. Dilute the sample until the measured  value is within the measuring range. For waters of unknown concentrations we recommend that you perform the test with widely different dilutions until the last dilution  confirms the previous value.

Reagents and accessories
Contents of reagents set:

• 20 test tubes R0
• 1 reagent R3
• 1 NANOFIX R2

Required devices:

• MACHEREY-NAGEL photometer
• Digital piston pipette 200–1000 µL (REF 91671) with pipette tips (REF 91667)
• Digital piston pipette 50–200 µL (REF 916914) with pipette tips (REF 916915)
• Tweezers for sampling NANOFIX capsules (REF 916114)

Sampling and preparation
See DIN EN ISO 5667-3-A 21.
Adjust to pH 4–9 prior to analysis.
Quality control
The measurement of a blank value and a standard is recommended before every measuring series as quality control measure.

Quality data:
The following data were determined during production according to ISO 8466-1 and DIN 38402-A51:

• Number of LOTs: 24
• Standard deviation of the method: ± 0.4 mg/L  °dH
• Coefficient of variation of the process: ± 4.2 %
• Confidence interval: ± 1.2 mg/L  °dH

Specified data for procedure:

• Sensitivity (absorbance of 0.010 A corresponds to): ± 0.2 mg/L  °dH
• Accuracy of a measurement value: ± 1.9 mg/L  °dH
• LOT-specific certificates are available at www.mn‑net.com.

Procedure

Determination of total hardness (method 0431–0434)

1. Open test tube

2. Add 1 NANOFIX R2

3. Seal test tube and shake vigorously

4. Open test tube again

5. Pipette 200 µL of sample into test tube

6. Seal test tube and shake vigorously

7. Wait 1 min

8. Clean outside of test tube

9. Measure
   Determination of calcium (method 0435, switch sub method)

10. Measurement against blank value (cuvette with measuring value total hardness instead of sample) is necessary

11. Open test tube

12. Add 200 µL R3

13. Seal test tube and shake vigorously

14.  Wait 1 min

15. Clean outside of test tube

16. Measure

17. Determination of magnesium (methode 0436)

18.  Measure (cuvette with measuring value calcium)

Disposal
Information regarding disposal can be found in the safety data sheet.
You can download the SDS from www.mn‑net.com/SDS.

Notes
When using a standard, the measured value is constant over a period of min. 30 min.
When using other photometers, make sure measurements are possible in test tubes (16 mm OD) and calibrate the method.
Test a sample of distilled water (REF 918932) to generate a blank value for the reagent.
Use the correction value when measuring cloudy or colored samples (see photometer handbook).
Information regarding safety can be found on the box’ label and in the safety data sheet. You can download the SDS from www.mn‑net.com/SDS.
04/2021

www.mn‑net.com Management System
EN ISO 13485:2016
ISO 9001:2015
www.tuv.com 
ID 9000008338
MACHEREY-NAGEL GmbH & Co. KG
Neumann-Neander-Str. 6–8
MACHEREY-NAGEL GmbH Neumann-Neander-Str. 6–8
52355 Düren · Germany
52355 Düren · Deutschland
DE Tel.: +49 24 21 969-0 info@mn-net.com
CH Tel.: +41 62 388 55 00 sales-ch@mn-net.com
FR Tel.: +33 388 68 22 68 sales-fr@mn-net.com
US Tel.: +1 484 821 0984 sales-us@mn-net.com

Read User Manual Online (PDF format)

Read User Manual Online (PDF format)  >>

Download This Manual (PDF format)

Download this manual  >>