BRESSER 9039001 Microscope Instruction Manual

MICROSCOPE
40X-1280X
Operating instructions

Art.No. 9039001

General Information

About This instruction Manual
Please read the safety instructions in this manual carefully. To avoid damage to the unit and the risk of injury, only use this product as described in the manual. Keep the instruction manual handy so that you can easily look up information on all the functions.
Intended Use
This product is intended only for private use.
It was developed for magnified viewing of natural and man-made objects.

General Warning
DANGER!
Tools with sharp edges and points are often used when working with this device. Because there is a risk of injury from such tools, store this device and all tools and accessories in a location that is out of the reach of children.
DANGER!
This device contains electronic components which operate via a power source (power supply). Only use the device as described in the manual, otherwise you run the risk of an electric shock.
DANGER!
Do not expose the device to high temperatures. Use only the supplied power supply or the recommended batteries. Do not short-circuit the device or batteries or throw them into a fire! Excessive heat or improper handling could trigger a short-circuit, a fire or an
explosion. Never bend, pinch or pull the power and connecting cables, extensions and adapters. Protect the cables from sharp edges and heat.
DANGER!
Children should only use the device under adult supervision. Keep packaging material, like plastic bags and rubber bands, out of the reach of children, as they pose a risk of choking.
Keep instructions and packaging as they contain important information.
Check the Power supply for damage to the cord, plug, enclosure or other parts on a regular basis before use. Do not use until the damage has been repaired. The toy is not intended for children under three years. The toy must only be used with the recommended
power supply. The power supply is not a toy. The toy is not to be connected to more than the recommended number of power supplies.
Power supply Specification:
Rated voltage: 5V
Input: 100-240V ~50/60Hz
Output: 5V/1A DC

CAREFUL!

Children must not have access to the included chemicals and liquids. Do not drink the chemicals. Wash hands thoroughly with running water after using the chemicals. In the event that the chemicals come into contact with your eyes or mouth, rinse thoroughly  with water. If you are in pain after  exposure, contact a doctor immediately and show him the substances.
NOTE!
Do not disassemble the device. In the event of a defect, please contact your dealer. The dealer will contact the Service Centre and can send the device in to be repaired, if necessary.
Do not expose the device to temperatures above 45 °C.

Operating instructions

Parts overview:

1. 10X WF eyepiece
2. 16X WF eyepiece
3. Barlow lens 2X
4. Eyepiece holder
5.  Microscope head
6. Objective revolver
7. Microscope stage
8.  Focus wheel
9.  LED lighting (transmitted light)
10.  Electricity supply
11. Microscope base
12.  Main plug
13.  5 slides, 10 covering glasses and 5 preparations in a plastic box
14.  Matted lens
15. Condenser lens
16.  Dimmer
17.  Colour filter disc
18.  LED lighting (reflected light)
19. Direct light/transmitted light switch
20. Microscope tools: a) pipette; b) tweezers
21. Magnifier box
22.  MicroCut
23.  Specimens: a) yeast; b) gum media (specimen inclusion medium); c) sea salt; d) brine shrimp eggs (Artemia)
24. Locking screw
25. Smartphone holder

General/Location

Make sure you position your microscope on a stable, solid surface.
An electricity supply is required for observation with the electric illuminator.
Position your device so that it can be disconnected from the power supply at any time.
The wall you use socket should be located near the device and easily accessible, since the plug on the power cord serves as a disconnecting device for the power supply. Always pull on the plug to separate the device from the power supply. Never pull on the cord.

 Electric LED lighting with dimmer

Before use, make sure the light switch (19) is set to ‘off’.
The microscope has two light sources. Lighting can be of three types. Set the switch (19) to ‘II’ to light the specimen from above (reflected light) or ‘I’ to light it from below (transmitted light). Use setting ‘III’ to light the specimen simultaneously by transmitted and reflected light. The transmitted light unit (9) is used for transparent specimens (those on glass slides). To view solid, non-transparent specimens, use the reflected light unit (18). Use of both forms of lighting simultaneously is only recommended for semitransparent specimens. This operating mode is not recommended for transmitted light specimens on slides as it may cause reflection on the slide.
To operate the supplied main power pack (12), first connect it to the microscope and then to a power socket (220-230V). Use the switch (19) to select the desired lighting mode and set the dimmer (16) to the desired brightness. As your device has continuously controllable lighting (dimmer), optimal illumination of the object to be viewed is guaranteed.

Colour filter disc

The colour filter (17) under the microscope table (7) aids in viewing very bright and transparent objects. Just select the right colour for the specimen in question. The components of colourless or transparent objects (e.g. starch particles, single-cell specimens) can thus be better recognised.

 Interchangeable illumination lenses

Your microscope comes with two illumination lenses (14 and 15). Depending on the object to be viewed, the proper lens should be attached to the LED illumination (9). The ground glass, or matted lens (14) is already mounted on the lighting unit. To change
the lenses, simply screw one off and the other on by turning the upper part of the transmitted light unit (9) (see also page 11).
An overview of lens purposes:
The matted lens (14) should be used for

• viewing extremely small items with the eyepieces (1 and 2) and the Barlow lens (3).
  The condenser lens (15) should be used for

• viewing standard items with the eyepieces (1 and 2) and the Barlow lens (3).

Microscope setup

The microscope head (5) will now be prepared for your first observation.
First, loosen the screw (24) and rotate the head into a convenient position.
Begin every observation with the lowest magnification.
Place the microscope’s table (7) with the focus knob (8) into the lowest position and rotate the objective revolver (6) until it locks on the lowest magnification (4X).

NOTE:
Make sure to place the microscope’s table (7) in its lowest  position before changing the objective in order to prevent damage to the microscope.
Insert the 10X eyepiece (No. 1, 1) in the Barlow lens (No. 1, 3).
Take care that the Barlow lens is inserted completely into the monocular head (No. 1, 4).

Observation

After you have set up the microscope with the proper illumination, the following prin-ciples are important:
Begin each observation at the lowest magnification, so that the centre and position of the object to be viewed is in focus. The higher the magnification, the more light is required for good picture quality.
Place a permanent slide culture directly under the microscope lens on the plate (7).
The specimen to be examined must be directly over the lighting.
Look through the eyepiece (1 and 2) and carefully turn the focus wheel (8) until you can see a sharp picture.
Now you can progress to a higher magnification. Slowly pull the Barlow lens (No. 2, 3) out of the monocular barrel (No. 2, 4). When the Barlow lens is nearly entirely pulled out, the magnification is raised to 2X.
For even higher magnification, you can put the 16X eyepiece (2) into the objective revolver (6) and rotate the objective revolver to a higher magnification (10X or 40X).
TIP:
Depending on the preparation, higher magnifications do not always lead to better pictures.
When changing the magnification of your microscope by changing or adjusting the  eyepiece, objective lens or Barlow lens, you must readjust the focus wheel (8) tosharpen the image.
NOTE:
Please be very careful when doing this. If you move the mechanical plate upward too fast, the objective lens and the slide can touch and become damaged.

 Condition and prepare viewed objects

7.1. Condition
With the Barlow lens nearly fully extended, your microscope’s magnification can be doubled. Both transparent and non-transparent specimens can be examined with this microscope, which features both direct and transmitted light. If opaque specimens are  being examined, such as small animals, plant parts, tissues, stones and the like, the light is reflected from the specimen, through the lens and eyepiece, where it is magnified, to the eye (reflected light principle, switch position I). If opaque specimens
are being examined, the light from below goes through the specimen, lens and eyepiece to the eye and is magnified en route (direct light principle, switch position II). Some small water organisms, plant parts and animal components are transparent by nature, but many others require pretreatment — that is, you need to make a thinnest possible slice of the object by hand cutting or using a microtome, and then examine this sample.

7.2. Creation of thin preparation cuts
Specimens should be sliced as thin as possible. A little wax or paraffin is needed to achieve the best results. Put the wax into a heat-safe bowl and heat it over a flame until the wax is melted. You can use a candle flame to melt the wax.
DANGER!
Be exremely carfeful when dealing with hot wax, as there is a danger of being burned.
Then, dip the specimen several times in the liquid wax. Allow the wax that encases the  specimen to harden. Use a MicroCut (22) or other small knife or scalpel to make verythin slices of the object in its wax casing.
DANGER!
Be extremely careful when using the MicroCut, knife or scalpel. These instruments are very sharp and pose a risk of injury.

Place the slices on a glass slide and cover them with another slide before attempting to view them with the microscope.
7.3. Creation of your own preparation
Put the object to be observed on a glass slide and cover the object with a drop of distilled water (No. 3) using the pipette (No. 3, 20a).
Set a cover glass (available at a well-stocked hobby shop) perpendicular to the edge of the water drop, so that the water runs along the edge of the cover glass (No. 4). Now lower now the cover glass slowly over the water drop.
TIP: The gum medium (23b) supplied is used to make permanent slide cultures. Add it instead of distilled water. The gum medium hardens so that the specimen is permanently affixed to its slide.

Experiments

Now that you’re familiar with your microscope’s functions and how to prepare slides,
you can complete the following experiments and observe the results under your microscope.
8.1. Newspaper print
Objects:
1. A small piece of paper from a newspaper with parts of a picture and some letters
2. A similar piece of paper from an illustrated magazine:
Use your microscope at the lowest magnification and make a slide preparation from each object. Place the slide with the newspaper on the microscope table and observe the slide. The letters in the newspaper appear broken because the newspaper is printed on raw, inferior paper. Now observe the slide with the magazine preparation. Letters of the magazine appear smoother and more complete. The picture from the newspaper consists of many small points, which appear somewhat dirty. The pixels (raster points) of the magazine image appear sharper.

8.2. Textile fibres
Objects and accessories:

1. Threads of different textiles: Cotton, linen, wool, silk, Celanese, nylon and any others you can find.
2. Two needles: Put each thread on a glass slide and fray each with the help of the two needles. Put a drop of water over each thread with the pipette and cover each with a cover glass. Adjust the microscope to a low magnification. Cotton fibres are of plant
   origin and look, under the microscope, like a flat, twisted band. The fibres are thicker and rounder at the edges than in the centre. Cotton fibres consist primarily of long, collapsed tubes. Linen fibres are also of plant origin; they are round and run in straight lines. The fibres shine like silk and exhibit numerous swellings along the shaft of the fibre. Silk is of animal origin and consists of solid fibres of smaller diameter than the hollow vegetable fibres. Each silk fibre is smooth and even and has the appearance of a small glass rod. Wool fibres are also of animal origin; the surface consists of overlapping scales, which appear broken and wavy. If possible, compare wool fibres from different weaving mills, and note the differences in the appearance of the fibres. Experts can determine the country of origin of wool based on its appearance under a microscope. Celanese is artificially manufactured by a long chemical process. All Celanese fibres show hard, dark lines on a smooth, shining surface. The fibres crinkle in the same way after drying. Observe the similarities and differences between the different fibres.

8.3. Saltwater brine shrimps Accessories:

1. Brine shrimp eggs (23d)
2. Sea salt (23c)
3. Magnifier box (21)
4.  Yeast (23a)

CAREFUL!
These eggs are not fit for human consumption.

8.3.1. Winter eggs of Artemia salina
Artemia salina are species of brine shrimp typically found in salt lakes — bodies of waterwith a higher salinity than even the ocean. During a drought, a salt lake can become ahostile habitat for organisms, and entire populations of Artemia salina sometimes dieoff. During drought conditions, to ensure that the species will repopulate the salt lakewhen the drought ends, Artemia salina lay thick-shelled eggs called winter eggs thatcan survive for up to ten years in a dormant state. Winter eggs can withstand heat, coldand chemicals. These eggs hatch when favourable conditions return to their ambientenvironment. The eggs provided (23d) are of this type.
8.3.2. Hatching winter eggs
To hatch the brine shrimps, create a solution with an appropriate salinity and tem- perature. First, fill two containers with a half litre of freshwater each, and let them both stand for about thirty hours. Next, pour half of the provided salt (23c) into one container and stir the solution until the salt dissolves. Pour some of this solution into the magnifier box (21). Place a few eggs close to the lid. Position the magnifier box somewhere with plenty of light but not in direct sunlight. The ambient temperature should ideally hover around 25 °C. As water in the magnifier box evaporates, gradually add freshwater from the second container. After two to three days, the eggs will hatch brine shrimp larvae, called nauplii.
8.3.3. Observing Artemia salina under a microscope
Using the pipette (20a), move some larvae from the container to a microscope slide for examination. When viewing the larvae, you’ll notice that they swim through the solution using hairlike limbs! Each day, examine a few more. You can even view the entire magnifier box under the microscope if you remove its lid. The larvae will mature in six to ten weeks, depending on the temperature of the water. Soon, you will have an entire generation of saltwater brine shrimps that reproduce frequently!
8.3.4. Feeding your Artemia salina
Feed your brine shrimps often to keep them alive. The best food is dry powdered yeast (23a). Give them some every other day. Be careful not to overfeed them, as doing so can cause the water to stagnate and poison the brine shrimps. If the water does begin to stagnate (you’ll see it darkening), transfer the brine shrimps to the fresh saline solution you have prepared earlier (see 8.3.2).

Smartphone holder

Open the flexible holder and put your smartphone in it. Close the cradle and make sure your phone is properly seated. The camera must be positioned exactly above the eyepiece. Open the locking clip on the back of the holder and fit the eyepiece view exactly onto your smartphone camera. Now retighten the locking clip and attach the smartphone holder to the eyepiece of your microscope. Now start the camera app. If the image is not yet centered on your display, loosen the locking clip slightly and readjust.
It may be necessary to use the zoom function to fill the image on the display. A slight shading at the edges is possible. Remove the smartphone from the cradle after use!
NOTE:
Make sure that the smartphone cannot slip off the cradle. Bresser GmbH accepts no liability for damage caused by a dropped smartphone!
Care and maintenance
Disconnect the unit from the power source (pull out the mains plug) before cleaning!
Only clean the outside of the unit with a dry cloth.

NOTE:
Do not use any cleaning fluid to avoid damaging the electronics.
Clean the lenses eyepieces and lenses only with a soft, lint-free cloth, like a microfibre cloth.
NOTE:
Do not apply excess pressure to the cloth so as to avoid scratching the lenses.
To remove more stubborn dirt, moisten the cleaning cloth with an eyeglass- cleaning solution and wipe the lenses gently. Protect the device from dust and moisture. After use, particularly in high humidity, let the device acclimatize for a short period of time, so that the residual moisture can dissipate before storing.

Troubleshooting
Problem
No picture visible

Solution

• Switch light on.
• Put condenser lens in place.
• Adjust focus.

Magnification table

EC Declaration of Conformity
Bresser GmbH has issued a “Declaration of Conformity” in accordance with applicable guidelines and corresponding standards. The full text of the EU declaration of conformity is available at the following internet address: www.bresser.de/download/9039001/CE/9039001_CE.pdf
UKCA Declaration of Conformity
**** Bresser GmbH has issued a „Declaration of Conformity“ in accordance with applicable guidelines and corresponding standards. The full text of the UKCA declaration of conformity is available at the following internet address: www.bresser.de/download/9039001/UKCA/9039001_UKCA.pdf

Bresser UK Ltd.
Suite 3G, Eden House, Enterprise Way • Edenbridge, Kent TN8 6HF • Great Britain
Disposal
Dispose of the packaging materials properly, according to their type, such as paper or cardboard. Contact your local waste-disposal service or environmental authority for information on the proper disposal.
Do not dispose of electronic devices in the household garbage! As per the Directive 2012/19/EC of the European Parliament on waste electrical and electronic equipment and its adaptation into German law, used electronic devices must be collected separately and recycled in an environmentally friendly manner.

Warranty

The regular warranty period is 2 years and begins on the day of purchase. To benefit from an extended voluntary guarantee period as stated on the gift box, registration on our website is required. You can consult the full warranty terms as well as information on
extending the warranty period and details  of our services at www.bresser.de/warranty_terms.

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TO LEARN MORE, VISIT NATGEO.COM/INFO

© National Geographic Partners LLC. All rights reserved.
NATIONAL GEOGRAPHIC and Yellow Border Design are trademarks of National Geographic Society, used under license.
Visit our website: www.nationalgeographic.com

Bresser GmbH
Gutenbergstr. 2
46414 Rhede
Germany
www.bresser.de • info@bresser.de
Bresser UK Ltd.
Suite 3G, Eden House
Enterprise Way • Edenbridge,
Kent TN8 6HF • Great Britain
Manual_9039001_Microscope_de-en-fr-nl-it-es-ru_NATGEO_v032023a
Irrtümer und technische Änderungen vorbehalten.
Errors and technical changes reserved.

References

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