MACHEREY-NAGEL Visocolor ECO Test Nitrate Kit Instructions

June 4, 2024
MACHEREY-NAGEL

MACHEREY-NAGEL Visocolor ECO Test Nitrate Kit Instructions
MACHEREY-NAGEL Visocolor ECO Test Nitrate Kit

Method

Nitrate ions are reduced to nitrite ions in an acidic medium. Combined with a suitable aromatic amine, these form an orange-yellow azo dye.

Measurement range

1–120 mg/L NO3

Contents of test kit (*refill pack)

sufficient for 110 tests

30 mL NO3-1
5 g NO3-2

1 measuring spoon 70 mm
2 screw-plug measuring glasses
1 slide comparator
1 color chart
1 plastic syringe 5 mL
1 instructions for use

Hazard warning

Information regarding safety can be found on the box’ label and in the safety data sheet. You can download the SDS from www.mn-net.com/SDS.

Instructions for use

also refer to the pictogram on the back of the color chart

  1. Pour a 5 mL water sample into each of the measuring glasses using the plastic syringe.
    Place a measuring glass on position A in the comparator.
    Only add the reagent to measuring glass B.

  2. Add 5 drops of NO3-1, seal the glass and mix.

  3. Add 1 level measuring spoonful of NO3-2, seal the glass and immediately shake the mixture well for 1 min.

  4. Open the glass after 5 min and place it on position B in the comparator.

  5. Slide the comparator until the colours match in the inspection hole on top. Check the measurement reading in the recess on the comparator reed. Mid-values can be estimated.

  6. After use, rinse out both measuring glasses thoroughly and seal them.

The reagents can be used for the photometric evaluation with photometer PF-12 / PF-12Plus.
This technique can be used also for analyzing sea water (see „Conversion table“).

Disposing of the samples

Information regarding disposal can be found in the safety data sheet. You can download the SDS from www.mn-net.com/SDS.

Interferences

Depending on their concentration, oxidizing substances may reduce the measurement reading or suppress the reaction totally. Chlorine ≤ 10 mg/L does not interfere.
Nitrite interferes (same reaction). This can be circumvented by addition of amido sulphonic acid (REF 918 973).
The water sample should be between 18 and 30 °C. At lower temperatures the reaction takes place at a significantly slower rate, and the results are limited.

Conversion table

mg/L NO3 – mg/L NO3-N (Nitrate in nitrogen) mmol/m3 mg/L NO3 in sea water
1 0.2 16 1
3 0.7 48 3
5 1.1 81 5
10 2.3 160 12
20 4.5 320 25
30 6.8 480 40
50 11 810 65
70 16 1130 95
90 20 1450 120
120 27 1940 160

Storage

Store the test kit in a cool (< 25 °C) and dry place.

MACHEREY-NAGEL GmbH & Co. KG · Valencienner Str. 11 · 52355 Düren · Germany
Tel.: +49 24 21 969-0 · info@mn-net.com · www.mn- net.com

References

Read User Manual Online (PDF format)

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