hygiena KIT230183 StarPrep One 8 Strip Kit Instruction Manual

hygiena KIT230183 StarPrep One 8 Strip Kit

Specifications

• Product Name : StarPrep One 8-Strip Kit
• Product Type: High-Throughput DNA extraction kit
• Product Number: KIT230183
• Designed for: 480 reactions

Product Information

The StarPrep One 8-Strip Kit is designed for high-throughput extraction of DNA from gram-negative bacteria for direct use in PCR. The kit includes all necessary components for efficient DNA extraction.

Product Usage Instructions

Required Materials
Ensure you have the following materials ready:

• 5 x 96 microtube rack with 8-tube strips 1.2 mL
• 5 bottles with 21 mL lysis buffer and a magnetic stir bar
• 10 bags with 12x 8-cap strips
• Reagent D (Product No. KIT230001)
• Sterile reservoir, 25 mL or 100 mL
• 8-Cap strips for microtube rack
• Deep well plate, 96 well, square well, PP, 1 mL
• Multichannel pipette and filter tips
• Centrifuge with swing-out rotor for microtiter plates capable
• TH 21 heating block thermostat
• Exchange block for deepwell plates for TH 21
• Lid weight with incubation frame for TH 21 heating block

Precautions and Preparations
Follow standard laboratory safety precautions when handling chemicals and biological samples. Ensure all equipment is clean and sterilized before use.

Workflows

• Extraction Procedure A: PCR Lyo
  Detailed instructions for PCR Lyo extraction procedure.

• Extraction Procedure B: PCR Liquid

  • Detailed instructions for PCR Liquid extraction procedure.

• Extraction Procedure C: Rapid
  

  • Detailed instructions for Rapid extraction procedure.

• Extraction Procedure D: Live/Dead
  Detailed instructions for Live/Dead extraction procedure using Reagent D.

FAQs

• Q: Can I use materials other than those specified in the instructions?
  A: It is highly recommended to only use the materials described in the manual to ensure optimal performance of the DNA extraction method.

• Q: How should I store the StarPrep One 8-Strip Kit?
  A: Store the kit components according to the storage conditions provided in the manual to maintain their integrity and effectiveness.

foodproof®
StarPrep One 8-Strip Kit
High-Throughput

PRODUCT INSTRUCTIONS
Documentation for the high-throughput extraction of DNA from gram-negative bacteria for direct use in PCR

Product No. KIT230183

foodproof®
StarPrep One 8-Strip Kit High-Throughput
Store kit at 15 to 25 °C FOR IN VITRO USE ONLY
Product No. KIT230183
Kit for 480 reactions

Product Instructions : Revision A, April 2024

 OVERVIEW

The foodproof® StarPrep® One 8-Strip Kit is designed for the rapid extraction of DNA from bacteria like Salmonella or Cronobacter for direct use in PCR. Up to 96 samples can be processed in parallel. The kit generates PCR template DNA from up to 100 µL (or more) of enrichment culture. For testing with a high background of dead bacteria, an additional live-dead treatment with Reagent D can be performed.
The extracted DNA can be used directly in any PCR application. The StarPrep One Lysis Buffer eliminates the need for hazardous organic extractions or chaotropic agents. The entire DNA preparation can be performed in a single tube, minimizing handling steps and exposure to hazardous material. The reduced number of handling steps saves time. Transfer steps of DNA-containing extracts are not necessary, thus cross-contamination risks are minimized.

General Information

• Number of Reactions
  The kit is designed for 480 reactions.

• Storage Conditions
  Store at 15 to 25 °C.
  The components of the foodproof StarPrep One 8-Strip Kit are guaranteed to be stable through the expiration date printed on the label.

• Applicability
  The lysis buffer can be used to prepare DNA from up to a 100 µL (or more) sample. The lysis buffer is optimized for the preparation of various types of sample material. The quality of the DNA obtained with the lysis buffer is suitable for any PCR application.

Kit Contents
A schematic representation of the foodproof StarPrep One 8-Strip Kit with all components:

• 1. 5 x 96 microtube rack with
• 8- tube strips 1.2 mL
• 2. 5 bottles with 21 mL lysis buffer and a magnetic stir bar
• 3. 10 bags with 12x 8-cap strips

INSTRUCTIONS

This section provides all information for a straightfoward DNA extraction from a variety of different food matrices.

Required Material
Most of the required equipment and reagents are available through Hygiena Diagnostics. Please contact usfor further information at www.hygiena.com/support.

It is highly recommended to only use the materials described below to ensure the performance of the method.

Reagents

Deep well plate, 96 well, square well, PP, 1 mL
Only for Procedure D (2.3.4) ****

Equipment

• Multichannel pipette and filter tips

  • e. g., 8-Channel Pipette, VIAFLO – INTEGRA Biosciences; GripTips: 50 to 1,250 µL
  • Or EP Xplorer Plus Electronic Multichannel Pipette; Filter Tips: 50 to 1,250 µL

• Centrifuge with swing-out rotor for microtiter plates capable of a > 5,400 × g centrifugal force e.g., Sigma 4-16S including rotor

• Or centrifuge with swing-out rotor for microtiter plates capable of a 2,000 × g centrifugal force e.g., Sigma 2-7 including rotor

• TH 21 heating block thermostat

• Exchange block for deepwell plates for TH 21

• Lid weight with incubation frame for TH 21 heating block thermostat

• Decapper 8-strip

• D-Light
  Product No. MCH230039

• Only for Procedure D (2.3.4)

Recommended:

• Magnetic stirrer
  e. g., Color squid wave – IKA®-Werke

• Cap installing tool

Precautions and Preparations

• Follow all universal safety precautions governing work with biohazardous materials, e.g. wear lab coats and gloves at all times. Properly dispose of all contaminated materials, decontaminate work surfaces, and use a biosafety cabinet whenever aerosols might be generated.
• For more information, please refer to the appropriate material safety data sheet (SDS). The SDS is available online at www.hygiena.com.
• Always use aersol barrier tips to avoid cross-contamination.
• To avoid foam formation of the lysis buffer, do not shake the bottles up and down. Mix thoroughly while pipetting the buffer for sample preparation. For mixing, use a magnetic stirrer at low speed to move the stir bar in the bottle or rotate the bottle before every pipetting step by moving it horizontally on the lab bench.
• To reach the required temperature of 95 to 100 °C in the tubes for the lysis step of the bacteria, the temperature of the corresponding heating unit TH 21 has to be set to 100 °C.
  • Thaw the Reagent D prior to use.
• Avoid more than three (3) freeze-thaw cycles. If necessary, aliquots can be prepared and stored according to the Reagent D manual. Avoid extended exposure to light.
  Only for Procedure D (2.3.4)

Workflows

• The following procedures describe the DNA isolation from enrichment culture using 8-strip tubes and multichannel pipettes.
• Procedure A (PCR Lyo) and B (PCR Liquid) are more sensitive protocols, where two different extraction methods are provided depending on which type of PCR kit is used for downstream processing (for lyophilized reagents: see
• PCR Lyo, for liquid reagents: see PCR Liquid).
• Procedure C (Rapid) is the fastest protocol suitable for less difficult matrices like milk powder.
• Procedure D (Live/Dead) contains a step for live and dead cell differentiation.

EXTRACTION PROCEDURE A: PCR LYO
This protocol is intended for extracts that will be used in combination with foodproof LyoKits.

1. SHAKE SAMPLE
   Shake enrichment culture gently and let suspension settle for 5 to 10 min.

2. ADD SAMPLE
   Transfer at least 100 µL sample (enrichment culture supernatant) to the 8-tube strips.

3. SEAL TUBES
   Seal the tubes with sterile cap strips.

4. CENTRIFUGE RACK
   Centrifuge 10 min at 5,400 x g (or 25 min at 2,000 x g).
   Make sure the rack is not sealed with rack lid during centrifugation.
   Note : Time and g-force depend on the centrifuge (Please see 2.1. Required Material for more information). Set the centrifuge acceleration to maximum speed and the brake to medium. If necessary, centrifugation forces should be calculated according to the centrifuge being used.

5. REMOVE CAPS
   Remove and discard the 8-cap strips from the 8-tube strips.
   To minimize the contamination risk, use the decapper 8-strip tool.

6. REMOVE SUPERNATANT
   Remove supernatant with a multichannel pipette immediately after centrifugation, discard and inactivate appropriately. Take care that the tips of the pipette in the reaction tubes are not touching the pellets.

7. PREPARE LYSIS BUFFER
   Dilute the needed lysis buffer 2:1 with sterile ddH2O in a sterile reservoir: 200 µL lysis buffer + 100 µL H2O per sample plus an additional 1 mL lysis buffer as dead volume.
   Note: Use a magnetic stirrer (low speed) or mix the bottle with lysis buffer gently immediately before use to avoid sedimentation of ingredients.

8. ADD LYSIS BUFFER
   Pipette lysis buffer up and down 5 to 10 times in reservoir before using it to avoid sedimentation of ingredients.
   Transfer 300 µL diluted lysis buffer with a multichannel pipette to each tube. Resuspend pellets by pipetting up and down 5 to 10 times.
   Note: For optimal DNA isolation efficiency, pellet has to be completely resuspended.

9. SEAL TUBES
   Seal the tubes tightly with new sterile cap strips.

10. INCUBATE
    Remove tube rack bottom and install incubation frame.
    Incubate rack with tube stripes 10 – 15 min at 100 °C in TH 21 Heating Block for 8-tube strips.
    Weight caps down with the lid weight.
    Note: To avoid removing and reinstalling the bottom, it is possible to place tube strips in an empty microtube rack (with rack bottom removed).

11.  CHILL
    Carefully remove the rack with the tube strips together with the lid weight from the heating unit and let it cool 3 – 5 min at room temperature.
    To avoid opening of caps, do not remove the lid weight until the strips have cooled down.

12. CENTRIFUGE RACK
    Reinstall tube rack bottom.
    Centrifuge 5 min at 5,400 x g (or 10 min at 2,000 x g).
    Make sure the rack is not sealed with rack lid during centrifugation.

SUPERNATANT FOR DETECTION
Use up to 25 µL of the extract for the respective foodproof PCR kit. Note: Strictly avoid transferring fractions of the sediment to the PCR reaction as this might cause PCR inhibition.
For later analysis, store DNA at -15 to -25 °C.
After thawing, mix briefly by vortexing and centrifuge at 2,000 × g for 5 min. Note: The sample is not purified. Proteins, RNA, and other materials remain in the sample. Long-term storage or archiving of prepared DNA samples is not recommended.

EXTRACTION PROCEDURE B: PCR LIQUID
This protocol is intended for extracts that will be used in combination with foodproof kits (liquid).

1. SHAKE SAMPLE
   Shake enrichment culture gently and let suspension settle for 5 to 10 min.

2. ADD SAMPLE
   Transfer at least 100 µL sample (enrichment culture supernatant) to the 8-tube strips.

3.  SEAL TUBES
   Seal the tubes with sterile cap strips.

4. CENTRIFUGE RACK
   10 min at 5,400 x g (or 25 min at 2,000 x g).
   Make sure the rack is not sealed with rack lid during centrifugation.
   Note: Time and g-force depend on the centrifuge (Please see 2.1. Required Material for more information). Set the centrifuge acceleration to maximum speed and the brake to medium. If necessary, centrifugation forces should be calculated according to the centrifuge being used.

5. REMOVE CAPS
   Remove and discard the 8-cap strips from the 8-tube strips.
   To minimize the contamination risk, use the decapper 8-strip tool.

6. REMOVE SUPERNATANT
   Remove supernatant with a multichannel pipette immediately after centrifugation, discard and inactivate appropriately. Take care that the tips of the pipette in the reaction tubes are not touching the pellets.

7. PREPARE LYSIS BUFFER
   Transfer required lysis buffer to a sterile reservoir.
   Use 200 µL lysis buffer per sample plus 1 mL lysis buffer as dead volume.
   Note: Use a magnetic stirrer (low speed) or mix the bottle with lysis buffer gently immediately before use to avoid sedimentation of ingredients.

8. ADD LYSIS BUFFER
   Pipette lysis buffer up and down 5 to 10 times in reservoir before using it to avoid sedimentation of ingredients.
   Transfer 200 µL lysis buffer with a multichannel pipette to each tube strip. Resuspend pellets by pipetting up and down 5 to 10 times.
   Note: For optimal DNA isolation efficiency, pellet must be completely resuspended.

9. SEAL TUBES
   Seal the tubes tightly with new sterile cap strips.

10. INCUBATE
    Remove tube rack bottom and install incubation frame.
    Incubate rack with tube stripes 10 – 15 min at 100 °C in TH 21 Heating Block for 8-tube strips.
    Weight caps down with the lid weight.
    Note: To avoid removing and reinstalling of the bottom, tube strips can be placed in an empty microtube rack (with rack bottom removed).

11. CHILL
    Carefully remove the rack with the tube strips together with the lid weight from the heating unit and let it cool 3 – 5 min at room temperature.
    To avoid opening of caps, do not remove the lid weight until the strips have cooled down.

12. CENTRIFUGE RACK
    Reinstall tube rack bottom.
    Centrifuge 5 min at 5,400 x g (or 10 min at 2,000 x g).
    Make sure the rack is not sealed with rack lid during centrifugation.

SUPERNATANT FOR DETECTION
Use 5 µL of the extract for the respective foodproof PCR kit.
Note: Strictly avoid transferring fractions of the sediment to the PCR reaction as this might cause PCR inhibition.
For later analysis, store DNA at -15 to -25 °C.
After thawing, mix briefly by vortexing and centrifuge at 2,000 × g for 5 min. Note: The sample is not purified. Proteins, RNA, and other materials remain in the sample. Long-term storage or archiving of prepared DNA samples is not recommended.

EXTRACTION PROCEDURE C: RAPID
This protocol is intended for rapid high-throughput extraction in combination with foodproof kits.
It is not suitable for all types of enrichment cultures. Please contact Hygiena Diagnostics GmbH for further information.

1. SHAKE SAMPLE
   Shake enrichment culture gently and let suspension settle for 5 to 10 min.

2.  PREPARE LYSIS BUFFER
   Transfer required lysis buffer to a sterile reservoir.
   Use 200 µL lysis buffer per sample plus 1 mL lysis buffer as dead volume.
   Note: Use a magnetic stirrer (low speed) or gently mix the bottle with lysis buffer immediately before use to avoid sedimentation of ingredients.

3. ADD LYSIS BUFFER
   Pipette lysis buffer up and down 5 to 10 times in reservoir before using it to avoid sedimentation of ingredients.
   Transfer 200 µL lysis buffer with a multichannel pipette to each tube.

4. ADD SAMPLE
   Transfer 25 to 50 µL sample (enrichment culture supernatant) to the 8-tube strips.

5. SEAL TUBES
   Seal the tubes tightly with sterile cap strips.

6. INCUBATE
   Remove tube rack bottom and install incubation frame.
   Incubate rack with tube stripes 10 – 15 min at 100 °C in TH 21 Heating Block for 8-tube strips.
   Weight caps down with the lid weight.
   Note: To avoid removing and reinstalling the bottom, tube strips can be placed in an empty microtube rack (with rack bottom removed).

7. CHILL
   Carefully remove the rack with the tube strips together with the lid weight from the heating unit and let it cool 3 – 5 min at room temperature.
   To avoid opening of caps, do not remove the lid weight
   until the strips have cooled down.

8. CENTRIFUGE RACK
   Reinstall tube rack bottom.
   Centrifuge 5 min at 2,000 x g.
   The rack must not be sealed with rack lid for centrifugation.
   Note: Time and g-force depend on the centrifuge (Please see 2.1. Required Material for more information). Set the centrifuge acceleration to maximum speed and the brake to medium. If necessary, centrifugation forces should be calculated according to the centrifuge being used.

SUPERNATANT FOR DETECTION
Use up to 25 µL of the extract for the respective foodproof PCR kit. Note: Strictly avoid transferring fractions of the sediment to the PCR reaction, because this might cause PCR inhibition.
For later analysis, store DNA at -15 to -25 °C.
After thawing, mix briefly by vortexing and centrifuge at 2,000 × g for 5 min. Note: The sample is not purified. Proteins, RNA, and other materials remain in the sample. Long-term storage or archiving of prepared DNA samples is not recommended.

EXTRACTION PROCEDURE D: LIVE/DEAD
This protocol is recommended for the detection of Enterobacteriaceae, or Enterobacteriaceae in combination with other parameter, e.g., Salmonella or Cronobacter. A step for live and dead cell differentiation with Reagent D (Product No. KIT230001) is included.

1. SHAKE SAMPLE
   Shake enrichment culture gently and let suspension settle for 5 to 10 min.

2. ADD SAMPLE
   Transfer 100 µL sample (enrichment culture supernatant) to the 96 deep well plate.

3. PREPARE REAGENT D
   Transfer an adequate volume of Reagent D into a sterile reservoir:
   Use 300 µL per sample and an additional 1 mL as dead volume.
   Note: The lights in the clean bench must be switched off. Proceed immediately with the following steps of the protocol. Avoid extended exposure to light.

4. ADD REAGENT D AND MIX
   Transfer 300 µL Reagent D with a multichannel pipette to each well of the deep well plate. Resuspend pellets by pipetting up and down 5 times.
   Note: For optimal DNA isolation efficiency, pellet must be completely resuspended. For uptake of Reagent D and mix, pipette with maximum speed of the automatic pipette. Proceed immediately with the following steps of the protocol. Avoid extended exposure to light.

5. D-LIGHT TREATMENT
   Place the 96 deep well plate in the D-Light unit.
   Incubate first in the dark for 5 min and subsequently expose to light for 5 min at room temperature in the D-Light unit.

6. TRANSFER VOLUME
   First resuspend 5 times and then transfer the whole volume (400 µL) with a multichannel pipette from the 96 deep well plate to 8-tube strips.

7. SEAL TUBES
   Seal the 8-tube strips tightly with sterile cap strips.

8. CENTRIFUGE
   10 min at > 5,400 x g (or 25 min at 2,000 x g).
   Make sure the rack is not sealed with rack lid during centrifugation.
   Note: Time and g-force depend on the centrifuge (Please see 2.1. Required Material for more information). Set the centrifuge acceleration to maximum speed and the brake to medium. If necessary, centrifugation forces should be calculated according to the centrifuge being used.

9. REMOVE CAPS
   Remove and discard the 8-cap strips from the 8-tube strips.
   To minimize the contamination risk, use the decapper 8-strip tool.

10. REMOVE SUPERNATANT
    Remove supernatant carefully with a multichannel pipette immediately after centrifugation, discard and inactivate appropriately.
    Take care that the tips of the pipette in the reaction tubes are not touching the pellets.

11.  PREPARE LYSIS BUFFER
    Transfer required lysis buffer to a sterile reservoir.
    Use 200 µL lysis buffer per sample plus 1 mL lysis buffer as dead volume.
    Note: Use a magnetic stirrer (low speed) or gently mix the bottle with lysis buffer immediately before use to avoid sedimentation of ingredients.

12.  ADD LYSIS BUFFER AND MIX
    Pipette lysis buffer up and down 5 to 10 times in reservoir before using it to avoid sedimentation of ingredients.
    Transfer 200 µL lysis buffer with a multichannel pipette to each tube. Resuspend pellets by pipetting up and down 5 to 10 times.
    Note: For optimal DNA isolation efficiency, pellet must be completely resuspended.

13. SEAL TUBES
    Seal the tubes tightly with new sterile cap strips.

14. INCUBATE
    Remove tube rack bottom and install incubation frame.
    Incubate rack with tube stripes 10 – 15 min at 100 °C in TH 21 Heating Block for 8-tube strips.
    Weight caps down with the lid weight.
    Note: To avoid removing and reinstalling the bottom, tube strips can be placed in an empty microtube rack (with rack bottom removed).

15. CHILL
    Carefully remove the rack with the tube strips together with the lid weight from the heating unit and let it cool 3 – 5 min at room temperature.
    To avoid opening of caps, do not remove the lid weight until the strips have cooled down.

16. CENTRIFUGE
    Reinstall tube rack bottom.
    Centrifuge 5 min at > 5,400 x g (or 10 min at 2,000 x g).
    Make sure the rack is not sealed with rack lid during centrifugation.

SUPERNATANT FOR DETECTION
Use up to 25 µL of the extract for the respective foodproof PCR kit. Note: Strictly avoid transferring fractions of the sediment to the PCR reaction because this might cause PCR inhibition.
For later analysis, store DNA at -15 to -25 °C.
After thawing, mix briefly by vortexing and centrifuge at 2,000 × g for 10 min. Note: The sample is not purified. Proteins, RNA, and other materials remain in the sample. Long-term storage or archival of prepared DNA samples is not recommended.

 Troubleshooting

• Perform a subcultivation, e.g., 1:10 dilution in fresh enrichment broth.
• Repeat DNA extraction with a reduced sample volume.

DNA extract contains too many PCR inhibitors.| Dilute DNA extract, e.g., 1:10, or reduce the amount of extracted DNA, e.g., for LyoKits 5 µL instead of 25 µL.
Some of the centrifugation pellet transferred over to the PCR.|

• Always centrifuge the DNA sample before performing PCR.
• Do not allow the filter tip to have contact with the pellet.

Supernatants are not completely removed.| Remove supernatants completely (e.g., after Reagent D treatment).
Low DNA yield| Improper storage of kit components.| Store kit reagents at 15 to 25 °C.
Enrichment culture contains substances that reduce the DNA extraction efficiency.| Perform a subcultivation or dilution, e.g., 1:10 in fresh enrichment broth.
Sample contains substances that reduce the DNA extraction efficiency.| Reduce the sample volume.
Not enough target organisms in enrichment culture.| Prolong the incubation phase.
Pellet resuspension incomplete.| Improve resuspension by prolonged pipetting or vortexing.
Suboptimal reaction conditions.|

• Ensure proper heating conditions.
• Verify correct temperature of the heating block with a thermometer.

Lid of the reaction tube opens during or after heating| Reaction tube not firmly closed or not enough weight exerted on the caps of the tube strips.|

• Ensure that all reaction tubes are firmly closed before heating.
• Weigh the caps down during heating and do not remove the weight until the tubes have cooled down.

Support
If you have questions or experience any problems with our products, please contact us:

www.hygiena.com/support

Our aim is to provide you with a solution as quickly and effectively as possible. We would also like you to contact us if you have any suggestions for improving the product or in case you would like to use our product for a different application. We highly value your feedback.

General Information

Quality Control
All products are regularly monitored by our quality control. You can find the certificate of analysis (COA) on our website. If you would like to carry out your own quality control, you will find the analysis method described in the certificate.

Waste  Disposal
All contaminated and potentially infectious material, like enrichment cultures or food samples, should be autoclaved before disposal and eliminated according to local rules and regulations. For proper disposal of unused chemicals, please refer to the SDS.

Warranty and Disclaimer of Liability
“ Limited Warranty” and “Disclaimer of Liability”: Hygiena Diagnostics GmbH warrants that this product is free from defects in materials and workmanship through the expiration date printed on the label and only if the following are complied with:

1. The product is used according to the guidelines and instructions set forth in the product literature;
2. Hygiena Diagnostics GmbH does not warrant its product against any and all defects when: the defect is as a result of material or workmanship not provided by Hygiena Diagnostics GmbH; defects caused by misuse or use contrary to the instructions supplied, or improper storage or handling of the product;
3. All warranties of merchantability and fi tness for a particular purpose, written, oral, expressed or implied, shall extend only for a period of one year from the date of manufacture. There are no other warranties that extend beyond those described on the face of this warranty;
4. Hygiena Diagnostics GmbH does not undertake responsibility to any purchaser of its product for any undertaking, representation or warranty made by any dealers or distributors selling its products beyond those herein expressly expressed unless expressed in writing by an offi cer of Hygiena Diagnostics GmbH;
5.  Hygiena Diagnostics GmbH does not assume responsibility for incidental or consequential damages, including, but not limited to responsibility for loss of use of this product, removal or replacement labor, loss of time, inconvenience, expense for telephone calls, shipping expenses, loss or damage to property or loss of revenue, personal injuries or wrongful death;
6. Hygiena Diagnostics GmbH reserves the right to replace or allow credit for any modules returned under this warranty.

Trademarks

• foodproof®, microproof®, vetproof®, ShortPrep®, StarPrep®, RoboPrep® and LyoKit® are registered trademarks of Hygiena Diagnostics GmbH.
• Hygiena® is a registered trademark of Hygiena.
• Other brand or product names are trademarks of their respective holders.

Reference Number
The reference number and original Hygiena Diagnostics GmbH article number: S 400 14 L

Change Index

• Version 1, April 2018:
• First version.
• Version 2, January 2019:
• Information for additional equipment added.
• Version 3, November 2019:
• New document design.
• Version 4, April 2021:
• Protocol for “Live/Dead” added.
• Revision A, April 2024
• Additional Rebranding updates, product number changes and formating.
• S 400 14L 20 -> INS-KIT230183-REVA

Hygiena® Camarillo, CA 93012 USA
diagnostics.support@hygiena.com
Manufactured by Hygiena Diagnostics GmbH Hermannswerder 17 14473 Potsdam
Germany
www.hygiena.com

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