hygiena GA21 Maize Detection Kit User Guide

Table of Contents

hygiena-GA21-Maize-Detection-Kit-PRODUCT

Specifications

Product Name: GMO GA21 Maize Detection Kit
Product No.: KIT230204
Revision: A, December 2023
PCR kit for the qualitative detection of GA21 DNA using real-time PCR instruments
For food testing purposes
Kit for 50 reactions
FOR IN VITRO USE ONLY

Program Setup

Data Interpretation

Verify the results of positive (Control Template) and negative (H2O) controls before interpreting the sample results.
Always compare samples to positive and negative controls.
Review data from each channel and interpret results as described in the table provided.

Preparation of the PCR Mix

Take appropriate precautions to prevent contamination, e.g., by using filter tips and wearing gloves.
Thaw reagents, mix (do not vortex!), and briefly spin vials before opening.
Prepare PCR mix for each reaction to a suitable tube (n samples + 2 controls + at least one additional reaction to cover pipetting loss).
Mix carefully but thoroughly by pipetting up and down.
Add PCR mix.
Add samples and controls.
Seal carefully.
Centrifuge briefly in a suitable centrifuge.
Start the real-time PCR run by cycling samples as described in the program setup.

FAQ

Q: Can this kit be used for human DNA detection?

A: No, this kit is specifically designed for the detection of GA21 DNA in maize samples for food testing purposes only.

Q: How many reactions can be performed with this kit?

A: This kit is designed for 50 reactions.
Product No. KIT230204
PCR kit for the qualitative detection of GA21 DNA using real-time PCR instruments.
Before starting, it is strongly recommended to read the entire product manual available on our website.

Select the following channels:

FAM (GA21) and VIC/HEX (Internal Control).
Pre-incubation: 1 cycle
- Step 1: 50 °C for 2 min
- Step 2: 95 °C for 10 min
Ampliﬁcation: 45 cycles
- Step 1: 95 °C for 15 sec
- Step 2: 60 °C for 60 sec
Fluorescence detection

Verify the results of positive (Control Template) and negative (H2O) controls, before interpreting the sample results.
Always compare samples to positive and negative controls.
Review data from each channel and interpret results as described in the table.

Take appropriate precautions to prevent contamination, e.g., using ﬁlter tips and gloves.
Thaw reagents, mix (do not vortex!), and brieﬂy spin vials before opening.

PREPARE PCR MIX
- Add 12.5 µL Master Mix (2XM ), for each reaction to a suitable tube.
- 4.0 μL Primer/Probe Mix (P) and 3.5 μL PCR-grade H2O (not included)
- (n samples + 2 controls + at least one additional reaction to cover pipetting loss).
- Mix carefully but thoroughly by pipetting up and down.
ADD PCR MIX
- Pipette 20 µL of prepared PCR mix into each strip or plate well.
ADD SAMPLES AND CONTROLS
- Pipette 5 µL of samples, negative control (PCR-grade H2O), or Control Template (C) into respective wells.
SEAL
- Carefully seal strips/plates.
CENTRIFUGE
- Brieﬂy spin strips/plate in a suitable centrifuge.
START REAL-TIME PCR RUN
- Cycle samples as described above.