hygiena KIT230224 Sheep Species Detection Kit User Guide

hygiena KIT230224 Sheep Species Detection Kit

Product Information

Specifications

• Product Name: Sheep Species Detection Kit
• Product No.: KIT230224
• Revision: A, December 2023
• PCR kit for the qualitative detection of Sheep DNA using real-time PCR instruments

Product Usage Instructions

Program Setup

Before setting up the PCR reactions, program your real-time PCR instrument by selecting the following channels: FAM (Sheep) and VIC/HEX (Internal Control).

Data Interpretation

Verify the results of positive (Control Template) and negative (H2O) controls before interpreting the sample results. Always compare samples to positive and negative controls. Interpret results based on the data from each channel.

Preparation of the RT-PCR Mix

1. Take appropriate precautions to prevent contamination, e.g., using filter tips and wearing gloves.
2. Thaw reagents, mix (do not vortex!), and briefly spin vials before opening.
3. Prepare PCR mix for each reaction in a suitable tube. Mix carefully but thoroughly.
4. Add PCR mix.
5. Add samples and controls.
6. Seal strips/plates accurately.
7. Briefly spin strips/plate in a suitable centrifuge.
8. Start real-time PCR run by cycling samples as described.

Note: For food testing purposes. For in vitro use only.

Frequently Asked Questions (FAQ)

Q: Can this kit be used for other species detection?

• A: This kit is specifically designed for Sheep DNA detection and may not be suitable for other species.

Q: How should I store the kit when not in use?

• A: Store the kit as per the storage instructions provided in the full product manual available on our website.
• Product No. KIT230224
• PCR kit for the qualitative detection of Sheep DNA using real-time PCR instruments.
• Before starting, it is strongly recommended to read the entire product manual available on our website.

PROGRAM SETUP

• Program your real-time PCR instrument before setting up the PCR reactions. Select the following channels:
• FAM (Sheep) and VIC/HEX (Internal Control).
• Pre-incubation: 1 cycle
  • Step 1: 95 °C for 10 min
• Amplification: 35 cycles
• Step 1: 95 °C for 15 sec
• *Step 2:** 61 °C for 40 sec
• Fluorescence detection

DATA INTERPRETATION

• Verify the results of positive (Control Template) and negative (H2O) controls, before interpreting the sample results.
• Always compare samples to positive and negative controls.
• Review data from each channel and interpret results as described in the table.

PREPARATION OF THE RT-PCR MIX

• Take appropriate precautions to prevent contamination, e.g., using filter tips and gloves.
• Thaw reagents, mix (do not vortex!), and briefly spin vials before opening.

1. PREPARE PCR MIX
   • Add 10 µL Master Mix (2XM) and for each reaction to 4μL Primer/Probe Mix (P) a suitable tube.
   • (In samples + 2 controls + at least one additional reaction to cover pipetting loss). Mix carefully but thoroughly by pipetting up and down.
2. ADD PCR MIX
   • Pipet 14 μL of prepared PCR mix into each strip or plate well.
3. ADD SAMPLES AND CONTROLS
   • Pipet 6 μL of samples, negative control (PCR-grade H2O, not included) or Control Template (C) into respective wells.
4. SEAL
   • Seal strips/plates accurately.
5. CENTRIFUGE
   • Briefly spin strips/plate in a suitable centrifuge.
6. START REAL-TIME PCR RUN
   • Cycle samples as described above.

• foodproof®
  • SL Sheep Species
• Detection Kit
  • KIT230224
• Kit for 50 reactions
  • Store kit at -15 to -25 °C
• For food testing purposes FOR IN VITRO USE ONLY
• Hygiena®
• Camarillo, CA 93012 USA
• diagnostics.support@hygiena.com
• www.hygiena.com

Documents / Resources

| hygiena KIT230224 Sheep Species Detection Kit [pdf] User Guide
KIT230224, KIT230224 Sheep Species Detection Kit, Sheep Species Detection Kit, Species Detection Kit, Detection Kit, Kit
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References

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