hygiena KIT230223 Bovine Species Detection Kit User Guide

hygiena KIT230223 Bovine Species Detection Kit

Product Information

Specifications

• Product Name: Bovine Species Detection Kit
• Product No.: KIT230223
• Revision: A, December 2023
• Application: PCR kit for the qualitative detection of Bovine DNA using real-time PCR instruments

Product Usage Instructions

Program Setup

• Program your real-time PCR instrument before setting up the PCR reactions.
• Select the following channels: FAM (Bovine) and VIC/HEX (Internal Control).
• Duration: 10 minutes
• Cycles: 1 cycle
• Step 1: 15 seconds
• Step 2: 40 seconds (Fluorescence detection)
• Total Cycles: 35 cycles

Data Interpretation

• Verify the results of positive (Control Template) and negative (H2O) controls before interpreting the sample results.
• Always compare samples to positive and negative controls. Review data from each channel and interpret results accordingly.

| FAM| VIC/HEX
---|---|---
Result Interpretation| Positive for Bovine| Negative for Bovine| Invalid

Preparation of the RT-PCR Mix

Take appropriate precautions to prevent contamination. Thaw reagents, mix (do not vortex!), and briefly spin vials before opening.

1. Prepare PCR Mix: Prepare for each reaction to a suitable tube. Mix carefully but thoroughly by pipetting up and down.
2. Add PCR Mix.
3. Add Samples and Controls.
4. Seal: Seal strips/plate accurately.
5. Centrifuge: Briefly spin strips/plate in a suitable centrifuge.
6. Start Real-Time PCR Run: Cycle samples as described above.

For food testing purposes. FOR IN VITRO USE ONLY.

FAQ

Q: Can this kit be used for detecting other species besides bovine?

• A: No, this kit is specifically designed to detect Bovine DNA using real-time PCR instruments.

Q: How should I store the kit when not in use?

• A: Store the kit per the instructions provided in the product manual on our website.
• PCR kit for the qualitative detection of Bovine DNA using real-time PCR instruments.
• Before starting, it is strongly recommended to read the entire product manual available on our website.

PROGRAM SETUP

• Program your real-time PCR instrument before setting up the PCR reactions. Select the following channels:
• FAM (Bovine) and VIC/HEX (Internal Control).
• Pre-incubation: 1 cycle Step 1: 95 °C for 10 min
• Amplification: 35 cycles Step 1 : 95 °C for 15 sec Step 2*: 61 °C for 40 sec

Fluorescence detection

DATA INTERPRETATION

• Verify the results of positive (Control Template) and negative (H2O) controls, before interpreting the sample results.
• Always compare samples to positive and negative controls.
• Review data from each channel and interpret results as described in the table.

PREPARATION OF THE RT-PCR MIX

• Take appropriate precautions to prevent contamination, e.g., using filter tips and gloves.
• Thaw reagents, mix (do not vortex!), and briefly spin vials before opening.

1. PREPARE PCR MIX
   • Add 10 μL Master Mix (2XM) and 4 μL Primer/Probe Mix (P) for each reaction to a suitable tube n samples + 2 controls + at least one additional reaction to cover pipetting loss).
   • Mix carefully but thoroughly by pipetting up and down.
2. ADD PCR MIX
   • Pipet 14 μL of prepared PCR mix into each strip or plate well.
3. ADD SAMPLES AND CONTROLS
   • Pipet 6 µL of samples, negative control (PCR-grade H2O, not included) or Control Template (C) into respective wells.
4. SEAL
   • Seal strips/plates accurately.
5. CENTRIFUGE
   • Briefly spin strips/plate in a suitable centrifuge.
6. START REAL-TIME PCR RUN
   • Cycle samples as described above.

• foodproof®
  • SL Bovine Species Detection Kit
• KIT230223
  • Kit for 50 reactions Store kit at -15 to -25 °C
  • For food testing purposes FOR IN VITRO USE ONLY
• Hygiena®
• Camarillo, CA 93012 USA
• diagnostics.support@hygiena.com
• www.hygiena.com

Documents / Resources

| hygiena KIT230223 Bovine Species Detection Kit [pdf] User Guide
KIT230223, KIT230223 Bovine Species Detection Kit, Bovine Species Detection Kit, Species Detection Kit, Detection Kit, Kit
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| hygiena KIT230223 Bovine Species Detection Kit [pdf] Instruction Manual
KIT230223 Bovine Species Detection Kit, KIT230223, Bovine Species Detection Kit, Species Detection Kit, Detection Kit, Kit

References

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